Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants

Andrucioli, Marcela Cristina Damião; Matsumoto, Mírian Aiko Nakane; Fukada, Sandra Yasuyo; Saraiva, Maria da Conceição Pereira; Bergamo, Ana Zilda Nazar; Romano, Fábio Lourenço; Silva, Léa Assed Bezerra da; Filho, Paulo Nelson

doi:10.1590/1678-7757-2018-0476

Cited by 17 publications

(15 citation statements)

References 31 publications

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“…On the other hand, it may primarily be caused by foreign body implantation. The bacterial infection could hamper the healing process and provide a poor body-seal environment on the neck of the TADs, further allowing for more bacterial invasion and triggering host inflammatory responses [ 11 , 38 ]. Our study showed that the dysbiosis of oral microbiota in failed TADs was not identical in each case.…”

Section: Discussionmentioning

confidence: 99%

Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)

Zhao¹,

Zhang

Guo³

et al. 2023

BMC Oral Health

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Background The stability of temporary anchorage devices (TADs) is critical in orthodontic clinics. The failure of TADs is multifactorial, and the role of the oral microbiome has not been clearly defined. Herein, we attempted to analyze the contribution of the oral microbiome to the failure of TADs. Methods Next-generation sequencing was adopted for analyzing the microbiome on the TADs from orthodontic patients. 29 TADs (15 failed TADs and 14 successful TADs) were used for 16S rRNA gene sequencing. A total of 135 TADs (62 failed TADs and 73 successful TADs) were collected to conduct metagenomic sequencing. Additionally, 34 verified samples (18 failed TADs and 16 successful TADs) were collected for quantitative real-time polymerase chain reaction analysis (qRT-PCR). Results Successful and failed TADs demonstrated discrepancies in microbiome structure, composition, and function. Clear separations were found in β-diversity in 16S rRNA gene sequencing as well as metagenomic sequencing (p < 0.05). Metagenomic sequencing showed that Prevotella intermedia, Eikenella corrodens, Parvimonas spp., Neisseria elongata, and Catonella morbi were enriched in the failed groups. qRT-PCR also demonstrated that the absolute bacteria load of Prevotella intermedia was higher in failed TADs (p < 0.05). Considering functional aspects, the failed group showed enriched genes involved in flagellar assembly, bacterial chemotaxis, and oxidative phosphorylation. Conclusions This study illustrated the compositional and functional differences of microorganisms found on successful and failed TADs, indicating that controlling bacterial adhesion on the surface of TADs is essential for their success rate.

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Section: Discussionmentioning

confidence: 99%

Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)

Zhao¹,

Zhang

Guo³

et al. 2023

BMC Oral Health

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“…Nevertheless, even when perfect hygiene is maintained and implantation properly performed, small fraction of patients may have a genetic predisposition to TADs failure, especially when they fail bilaterally [ 13 ]. Andrucioli et al [ 31 ] evaluated the gene expression of proinflammatory cytokines and osteoclastogenesis mediators in peri-miniscrew gingival tissue samples using real-time polymerase chain reaction, to verify if gingival inflammation and bone resorption could be associated with implant failure. They concluded that the higher IL-6 expression could be associated with miniscrew failure, since a prolonged excessive release of IL-6 was translated into persistent oral inflammation and tissue destruction via proteases, osteoclasts and methylation changes.…”

Section: Discussionmentioning

confidence: 99%

Evidence-based selection of orthodontic miniscrews, increasing their success rate in the mandibular buccal shelf. A randomized, prospective clinical trial

et al. 2022

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Background Skeletal anchorage has made it possible to perform complex orthodontic tooth movements that are difficult or even impossible to achieve with conventional orthodontic treatment. Mandibular buccal shelf miniscrews, used for distalization, play a particularly important role in treatment of Class III malocclusion. Unfortunately, stability of the miniscrews placed in the mandible is still considered at higher risk of failure compared to other intraoral locations. The aim of our study was to determine the influence of the miniscrew size on their long-term stability, occurrence of oral mucosa inflammation and pain lasting over 48 h after implantation. Methods 184 Absoanchor® miniscrews (Dentos, South Korea) in two sizes: SH2018-10 (length 10 mm, ø 1.8–2.0 mm) and SH1514-08 (length 8 mm, ø 1.4–1.5 mm) were inserted in the mandibular buccal shelf in 92 Caucasians aged 20–50 years, diagnosed with Class III malocclusion that required en-masse distalization of the mandibular dentition. Data was statistically analyzed with the level of significance set at p = .05. Results 91.3% of the SH2018-10 and 75% of the SH1514-08 miniscrews were stable, and this difference was statistically significant (p < .05). Inflammation of the oral mucosa was noticed around both types of miniscrews and affected 50% of the SH2018-10 and 26.09% of the SH1514-08 group (p < .05). Pain lasting longer than 48 h after implantation was related to 60.87% and 20.65% of the SH2018-10 and the SH1514-08 miniscrews (p < .05), respectively. Inflammation associated with larger SH2018-10 miniscrews did not affect their stability (p > .05), contrary to the SH1514-08 ones (p < .05). When inflammation was present, the overall success rate declined to 64.29%, from 94.74% noted for TADs without inflammation. According to the log-rank test, smaller TADs failed significantly sooner than the larger ones (p = .002). Conclusion Larger SH2018-10 miniscrews are the anchorage of choice for the mandibular buccal shelf, despite triggering inflammation and long-lasting pain significantly more often than the smaller ones. Therefore, this issue should be discussed with every patient prior to miniscrew use. Trial registration ID: ClinicalTrials.gov Identifier: NCT05280678 Date of Registration: 15/03/2022. Retrospectively registered.

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“…The expressions of pro-inflammatory cytokines including TNF-α, IL-6 and IL-1β were significantly elevated in AD model. TNF-α is released by lymphocytes, fibroblasts, leukocytes and epithelial cells and functions mainly by interacting with the Tumor necrosis factor receptors (TNFRs) [2]. TNF-α is a critical pro-inflammatory cytokine which activates the subsequent inflammatory response by triggering various signaling pathways and stimulates the expressions of IL-6 and IL-1β, these cytokines ultimately resulted in inflammation and activation of NF-κB [15].Previous studies have suggested that neuroinflammatory response in the brain is a significant pathological feature of AD [19].…”

Section: Discussionmentioning

confidence: 99%

Schisandrin ameliorates cognitive deficits, endoplasmic reticulum stress and neuroinflammation in streptozotocin (STZ)-induced Alzheimer’s disease rats

et al. 2020

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Schisandrin, an active component extracted from Schisandra chinensis (Turcz.) Baill has been reported to alleviate the cognitive impairment in neurodegenerative disorder like Alzheimer's disease (AD). However, the mechanism by which schisandrin regulates the cognitive decline is still unclear. In our study, intracerebroventricular injection of streptozotocin (STZ) was employed to establish AD model in male Wistar rats, and indicated dose of schisandrin was further administered. The Morris water maze test was performed to evaluate the ability of learning and memory in rats with schisandrin treatment. The results indicated that schisandrin improved the capacity of cognition in STZ-induced rats. The contents of pro-inflammatory cytokines in brain tissue were determined by ELISA, and the expressions of these cytokines were assessed by western-blot and immunohistochemistry. The results showed that treatment of schisandrin significantly reduced the production of inflammation mediators including tumor necrosis factor-α, interleukin-1β and interleukin-6. Further study suggested a remarkable decrease in the expressions of ER stress maker proteins like C/EBP-homologous protein, glucose-regulated protein 78 and cleaved caspase-12 in the presence of schisandrin, meanwhile the up-regulation of sirtuin 1 (SIRT1) was also observed in the same group. Additionally, the results of western-blot and EMSA demonstrated that schisandrin inhibited NF-κB signaling in the brain of STZ-induced rats. In conclusion, schisandrin ameliorated STZ-induced cognitive dysfunction, ER stress and neuroinflammation which may be associated with up-regulation of sirtuin 1 (SIRT1). Our study provides novel mechanisms for the neuroprotective effect of 3 schisandrin in AD treatment.

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Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants

Cited by 17 publications

References 31 publications

Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)

Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)

Evidence-based selection of orthodontic miniscrews, increasing their success rate in the mandibular buccal shelf. A randomized, prospective clinical trial

Schisandrin ameliorates cognitive deficits, endoplasmic reticulum stress and neuroinflammation in streptozotocin (STZ)-induced Alzheimer’s disease rats

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