Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

Abstract

Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses.ObjectiveTo assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation.Material and methodsWe synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method.ResultsTwelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%.ConclusionsTwelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.

Strategies to Reduce Biofilm Formation in PEEK Materials Applied to Implant Dentistry—A Comprehensive Review

“…Among the studies included in this review describing strategies for PEEK modification through the incorporation of antibiofilm agents, the embedding of lactams through the PEEK sulfonation process is worth mentioning [45]. Lactams are compounds analogous to furanones, which were initially isolated from the algae Delisea pulchra, and had been proved to be effective against Streptococus mutans biofilms [77]. An in vitro study [26] demonstrated that PEEK sulfonation positively interferes with the ability of fibroblasts L929 to spread over the surface of the material [26].…”

Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

“…To the best of the authors' knowledge, no studies have been found using Fn3 against bacterial biofilms. Nonetheless, chemical analogues to Fn3, such as mucochloric acid and other derivatives, have been found to have an effect against early biofilm formation of B. subtilis [23], Streptococcus mutans [54] and Pseudomonas aeruginosa [55], among others. Nevertheless, this is the first report testing the effects of different dosages along time of 3,4-dichloro-2(5H)-furanone against L. monocytogenes biofilms.…”

Effects of Synthetic Tetronamides and Methylated Denigrins on Bacterial Quorum Sensing and Biofilm Formation

“…Over the past years, motivated by the findings of butenolides as biofilm inhibitors, our groups have synthesized different analogues of natural butenolides and tested their antibiofilm activities. − Among them, a rubrolide analogue 3 and its lactam derivative 4 reduced the biofilm formation of P. aeruginosa with IC 50 values of 3.9 and 0.6 μg/mL, respectively …”