Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

Abstract

Furanones are analogues of acylated homoserine lactones with proven antifouling activity in both Gram-positive and Gram-negative bacteria though the interference of various quorum sensing pathways. In an attempt to find new strategies to prevent and control Listeria monocytogenes biofilm formation on stainless steel (SS) surfaces, different concentrations of six synthetic furanones were applied on biofilms formed by strains isolated from food, environmental, and clinical sources grown onto AISI 316 SS coupons. Among the furanones tested, (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone and 3,4-Dichloro-2(5H)-furanone significantly (p < 0.05) reduced the adhesion capacity (>1 log CFU cm−2) in 24 h treated biofilms. Moreover, individually conducted experiments demonstrated that (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone was able to not only significantly (p < 0.05) prevent L. monocytogenes adhesion but also to reduce the growth rate of planktonic cells up to 48 h in a dose-dependent manner. LIVE/DEAD staining followed by epifluorescence microscopy visualisation confirmed these results show an alteration of the structure of the biofilm in furanone-treated samples. Additionally, it was demonstrated that 20 µmol L−1 of 3,4-Dichloro-2(5H)-furanone dosed at 0, 24 and 96 h was able to maintain a lower level of adhered cells (>1 log CFU cm−2; p < 0.05). Since furanones do not pose a selective pressure on bacteria, these results represent an appealing novel strategy for the prevention of L. monocytogenes biofilm grown onto SS.

Exploring an Efficient and Eco-Friendly Signaling Molecule and Its Quorum Quenching Ability for Controlling Microcystis Blooms

“…In addition, furanones as green and safe quorum quenchers have been widely used in food preservation and aquaculture disease resistance and did not induce drug resistance. 50 The above results suggest that FN was the most effective and eco-friendly inhibitor for controlling cyanobacterial blooms.…”

“…For instance, FB produced by Delisea pulchra is the most widely used active substance with a furanone structure for quorum quenching Table summarizes the present application of these molecules as QSIs and as controllers of biofilm formation, motility, and violacein synthesis. − At this point, these molecules have not been used to treat cyanobacterial blooms.…”

Architectural Features and Resistance to Food-Grade Disinfectants in Listeria monocytogenes-Pseudomonas spp. Dual-Species Biofilms

“…De visu analysis of the epifluorescence microscopy images of monospecies L. monocytogenes 48 h biofilms grown on AISI 316 SS revealed a variety of growth on the coupon ( Figure 1 ). Of note, all the L. monocytogenes strains used in the present study were chosen since a previous work demonstrated no statistically significant differences in the level of adhesion in terms of CFU cm –2 ( Rodríguez-López et al, 2019a ). Consequently, differences in the biofilm morphology, such as occupied area, would be attributed to factors other than the amount of viable-and-cultivable cells in the different biofilms.…”

Exploring communication signals inside the microbial community of a Listeria monocytogenes-carrying biofilm contamination site

“…The list of strains used in this work are included in Table 1. All bacterial species characterized in a previous study from L. monocytogenes-harboring contamination sites located in food industry (Rodríguez-López et al, 2019a).…”

“…Improper hygienic conditions and the high resistance of biofilms formed on surfaces of food processing environments are considered the main causes of L. monocytogenes increases the probability of food contamination (Mazaheri et al, 2021). Several studies have shown that L. monocytogenes survives in food processing facilities by forming or integrating into multispecies microbial communities at contamination sites (Galié et al, 2018;Rodríguez-López et al, 2019a). Moreover, even after cleaning and disinfection, L. monocytogenes-containing polymicrobial biofilms have been detected (Carpentier and Chassaing, 2004;Rodríguez-López et al, 2015).…”