“…Several research studies try to elucidate the cause of the impaired performance in broilers fed diets with reduced protein levels. The hypotheses include: decreases in digestible glycine levels (Namroud et al, 2010;Vasconcellos et al, 2015Vasconcellos et al, , 2011Yuan et al, 2012); decreases in digestible lysine levels (Saima, Jabbar, Mehmud, Abbas, & Mahmood, 2010); decreases in digestible glycine/threonine ratio (Waldroup, Jiang, & Fritts, 2005b); changes in electrolyte balance (Han, Suzuki, Parsons, & Baker, 1992;Vieira et al, 2015); decreases in the ratio between essential and non-essential amino acids (Waldroup et al, 2005a); reductions in dietary nitrogen levels (Bregendahl, Sell, & Zimmerman, 2002); reductions in feed intake (Namroud et al, 2010); changes in serum levels of triiodothyronine, thyroxine and ammonia (Carew, Evarts, & Alster, 1998;Namroud et al, 2008Namroud et al, , 2010; changes in the concentration of intestinal transporters, as well as in the absorption efficiency (Corzo, Loar, Kidd, & Burgess, 2011;Nyachoti, Omogbenigun, Rademacher, & Blank, 2006;Tuitoek, Young, de Lange, & Kerr, 1997;Wu et al, 2015) and increases in fat content in the carcass (Yamazaki, Murakami, & Takemasa, 1998), which reduces muscle tissue deposition.…”