Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Salina, A.; Timenetsky, Jorge; Barbosa, Maysa Santos; Azevedo, Cristiane M.; Langoni, Hélio

doi:10.1590/1678-5150-pvb-6259

Cited by 7 publications

(5 citation statements)

References 35 publications

(46 reference statements)

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“…We showed here that milk from udders with Mycoplasma mastitis tended to have greater SCC when compared to udders with non-Mycoplasma mastitis or a healthy udder. This is in consistent with previous studies that have linked the high somatic cell content to the prevalence of Mycoplasma mastitis [38,39,58]. We also showed that both Mycoplasma mastitis and non-Mycoplasma mastitis induced significant changes in the milk components.…”

Section: Discussionsupporting

confidence: 93%

Impact of udder infections on biochemical composition of milk in context of pesticides exposure

Ali¹,

Ali²,

Abd-Algawad

et al. 2022

Vet World

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Background and Aim: Environmental contaminants such as pesticides have shown immunomodulatory effects that can make animals highly susceptible to pathogenic invasion. The current work aims to study the incidence of udder infections in a single dairy herd of 160 cows in Qalyoubia Governorate, in relation to the potential intoxication of dairy cattle with organochlorine (OCs) pesticides. The study also aims to investigate the impact of udder infections on milk composition. Materials and Methods: The dairy herd was screened for udder infections using the California mastitis test and measurement of somatic cell count (SCC), followed by bacteriological and molecular analysis. In parallel, the milk samples were also tested for residues of 15 OCs compounds using gas chromatographic analysis. Results: The examined herd showed a high prevalence of mastitis (37.5%) and Mycoplasma was identified as the main bacterial pathogen. OCs residues were detected in milk of 45 cows out of 160 with a higher incidence in mastitic (43.3%) than in healthy cows (19%). Further, the biochemical analysis of milk showed a significant drop in major electrolytes combined with a significant rise in blood-borne electrolytes (Na and Cl) and total protein. This was more extreme in the case of Mycoplasam mastitis compared to non-Mycoplasma mastitis. In addition, Mycoplasma mastitic milk revealed a high level of malondialdehyde associated with reduced antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase), compared to non-Mycoplasma mastitis. Conclusion: Mycoplasma mastitis was shown to be associated with increased SCC and, in turn, appeared significantly correlated with increased biochemical changes in milk, indicating the serious impact of Mycoplasma mastitis on the dairy industry. Our data also show a strong correlation between increased SCC and biochemical changes in milk, suggesting that tested biochemical parameters might serve as potential biomarkers for the early detection of mastitis. The study also suggested a potential relationship between poisoning with OCs and susceptibility to bacterial udder infections. However, further studies are required to examine the immune status of a dairy herd in relation to the level of OCs in cow's blood, as well as the water sources used, grass forage and soil.

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Section: Discussionsupporting

confidence: 93%

Impact of udder infections on biochemical composition of milk in context of pesticides exposure

Ali¹,

Ali²,

Abd-Algawad

et al. 2022

Vet World

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“…Забір крові проводили для визначення в сироватці крові концентрації Среактивного білка (СРБ), глюкози, креатиніну, молочної та піровиноградної кислот; досліджували активність таких ферментів як аспартатамінотрансфераза (АСТ; КФ 2.6.1.1), аланінамінотрансфераза (АЛТ; КФ 2.6.1.2). Цитохімічними методами в лімфоцитах крові визначали активність: сукцинатдегідрогеназа (СДГ; КФ 1.3.99.1) (Salina et al, 2020), гліцерол-3-фосфат дегідрогеназа (ГФДГ; КФ 1.1.99.5) та НАДНдегідрогеназа (НАДНД; КФ 1.6.5.3) (Salina et al, 2020). Для оцінки статистично значущих змін тварин після завершення терапії був використанний непараметричний критерій знаків G (Salina et al, 2020).…”

Section: матеріал і методи дослідженьunclassified

“…Цитохімічними методами в лімфоцитах крові визначали активність: сукцинатдегідрогеназа (СДГ; КФ 1.3.99.1) (Salina et al, 2020), гліцерол-3-фосфат дегідрогеназа (ГФДГ; КФ 1.1.99.5) та НАДНдегідрогеназа (НАДНД; КФ 1.6.5.3) (Salina et al, 2020). Для оцінки статистично значущих змін тварин після завершення терапії був використанний непараметричний критерій знаків G (Salina et al, 2020).…”

Section: матеріал і методи дослідженьunclassified

“…1). Для оцінки статистично значущих змін тварин після завершення терапії був використаний непараметричний критерій знаків G (Salina et al, 2020). Для апробації нової схеми лікування маститу у корів була відібрана корова під номером 3095 з характерними клінічними ознаками катарального маститу задньої лівої долі вимені (підвищення місцевої температури, болючість та ущільнення вимені, зміна кольору та консистенції молока, наявність пластівців і згустків (рис.…”

Section: результати та їх обговоренняunclassified

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A clinical case of catarrhal mastitis in a cow

Tarnavskyi

Hirin²,

Hulii³

et al. 2022

SMLNUVMB

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Food security includes not only the provision of basic foodstuffs to the population but also the guarantee of their quality and safety for the consumer. These criteria are essential for milk as the primary food product. The pathological processes, which are developed in the breast, harm the quality of milk, and the medical products used in their treatment, may affect the safety of this product. The article has represented the results of the bioregulatory approach in treating catarrhal mastitis in cows using medical products containing ultra-low doses of biologically active substances. These products do not have a withdrawal period regarding milk. The use of this approach makes it possible to increase the safety of treatment, which in turn will lead to economic benefits. Clinical symptoms of mastitis were determined by clinical signs (increasing local temperature, soreness, thickening of the udder, changing in color and consistency of milk, presence of flakes and clots). A сow No. 3095 was selected with characteristics of clinical symptoms of catarrhal mastitis of the rear left lobe of the udder. The following scheme was used for therapy: intramuscular injection of Traumeel 10 ml once a day, intracisternal injection of Lachesis Сompositum 10 ml into the affected part once a day, Traumeel(gel) locally after milking. The choice of bioregulatory preparations was based on the components and their action. On the third day, the cow's udder swelling decreased, local temperature normalized. The color and consistency of milk during control milking and its examination with the Avita diagnostic test showed an improvement from (+++) to (++). Complete clinical and laboratory recovery was diagnosed on the sixth day. A cow was returned to the general herd. Based on the treatment results, it can be concluded that this treatment scheme for catarrhal mastitis is effective. The absence of a withdrawal period regarding milk and the acceleration of recovery rates make it economically profitable.

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“…Therefore, PCR techniques targeting the 16S rRNA gene do not reliably distinguish between them unless additional methods are used such as melting temperature analysis of the PCR products, denaturing gradient gel electrophoresis (DGGE) of the amplicons [64], the use of different spp. primers [65], DNA sequencing [25]. Recently, DNA sequencing based on 16S rRNA sequence analysis can distinguish between 130 different Mycoplasma spp.…”

Section: Isolation and Identification Of Mycoplasma Bovismentioning

confidence: 99%

Mycoplasma bovis: Taxonomy, Characteristics, Pathogenesis and Antimicrobial Resistance

Ammar

El-Hamid

Hashem

et al. 2021

Zagazig Veterinary Journal

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Mycoplasma bovis (M. bovis) is one of the most significant bacteria, which leads to multiple bovine diseases such as keratoconjunctivitis, otitis media, arthritis, genital disorders, mastitis, and pneumonia in cattle. M. bovis is considered the second most pathogenic mycoplasmas infection after Mycoplasma mycoides sub sp. mycoides (Mmm) that causes contagious bovine pleuropneumonia. M. bovis is a wall-less host-specific bacterium. Currently, it is responsible for important economic problems worldwide such as reducing production, premature culling, and increasing the mortality of the affected animals. The infection caused by M. bovis is hampered due to the lack of effective vaccines and treatment. Besides, antibiotic resistance to macrolides and fluoroquinolones, the drug of choice for M. bovis treatment, has formidable economic losses due to treatment limitations by these antimicrobials. Our review highlights and discusses the taxonomy, general characteristics, economic importance, isolation, identification, and pathogenesis of M. bovis. Finally, it focused on the antimicrobial resistance of this particular bacterium.

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Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Cited by 7 publications

References 35 publications

Impact of udder infections on biochemical composition of milk in context of pesticides exposure

Impact of udder infections on biochemical composition of milk in context of pesticides exposure

A clinical case of catarrhal mastitis in a cow

Mycoplasma bovis: Taxonomy, Characteristics, Pathogenesis and Antimicrobial Resistance

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