Basal levels of inorganic elements, genetic damages, and hematological values in captive Falco peregrinus

Abstract

It is essential to determine the basal pattern of different biomarkers for future evaluation of animal health and biomonitoring studies. Due to their great displacement capacity and to being at the top of their food chains, birds of prey are suitable for monitoring purposes. Furthermore, some birds of prey are adapted to using resources in urban places, providing information about this environment. Thus, this study determined the basal frequency of micronuclei and other nuclear alterations in peripheral blood erythrocytes of Falco peregrinus . Hematological and inorganic elements analysis were also performed. For this purpose, 13 individuals (7 females and 6 males) were sampled in private breeding grounds. Micronucleus, nuclear buds, nucleoplasmic bridges, notched nuclei, binucleated cells and nuclear tails were quantified. Inorganic elements detected included the macro-elements Ca, P, Mg, Na, Cl, S and K as well as the micro-elements Fe, Al and Zn. Our study found similar values compared to previous studies determining the reference ranges of hematologic parameters in falcons. The only different value was observed in the relative number of monocytes. Thus, this study is the first approach to obtaining reference values of cytogenetic damage in this species and could be useful for future comparisons in biomonitoring studies.

Micronúcleos: Actualización del papel en la inestabilidad genética, inflamación, envejecimiento y cáncer. Revisión panorámica.

“…Dependiendo de la especie se debe considerar la histología del bazo, ya que el sinusoidal elimina los eritrocitos micronucleados (EMN) de sangre periférica casi al 100%, como sucede en el humano; en contraste, el bazo no sinusoidal no es tan eficiente en retirar los EMN, por ello en sangre periférica se pueden observar altas frecuencias de EMN espontáneos, como en el ratón. La prueba de EMN en sangre periférica requiere de solo unas gotas de sangre, hacer dos frotis (el de trabajo y el de respaldo) el conteo es sobre 10, 000 eritrocitos totales (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16).…”

Integrating Deep Learning into Genotoxicity Biomarker Detection for Avian Erythrocytes: A Case Study in a Hemispheric Seabird

“…Detection of nuclear abnormalities in erythrocytes has been performed in the last decade as the main procedure to assess genotoxicity in birds of different taxa [1][2][3]. Increases in nuclear abnormalities can be triggered by exposure of birds to different types of contaminants [4,5].…”