Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities

Abstract

This study was designed to investigate chemical composition, the total phenolic content, flavonoid content, antioxidant activity and to analyze through FT-IR spectroscopy method the freeze-dried extract of Ecballium elaterium fruit from three different localities. The highest level of phenolic and flavonoid contents was recorded for the fruit juice from the Cap-Bon region, with 106.4 ± 0.4 mg GAE/g and 6.5 ± 0.2 mg QE/g, respectively. Antioxidant activity varied in dose-dependent manner with IC 50 values for DPPH scavenging of the freeze-dried fruit juice extracts from Cap-Bon, Kef and Sidi Bouzid were 38.6 ± 0.2, 50.1 ± 0.7, and 50.7 ± 0.2 µg/mL, respectively. The results from the FRAP test showed that the freeze-dried extracts of Cap-Bon exhibited potent activity, followed by those from Kef and Sidi Bouzid. Similar trend were revealed for ABTS•+ test, with the fruit juice extract from Cap-Bon (IC 50 = 0.6 ± 0.0 mg/mL). Furthermore, a good positive correlation was observed between the total phenols and three assays, especially DPPH. The freeze-dried extracts of fruit juice from Cap-Bon showed strong ability to act as antioxidants and can be considered as promising natural source of bioactive compounds. FT-IR analysis of each freeze-dried extract confirmed its richness on polyphenols and biological active functional groups.

A comparative study on chemical composition, antibiofilm and biological activities of leaves extracts of four Tunisian olive cultivars

“…The results on total phenols and flavonoids contents support the observed activity of the extracts against free radicals (Felhi et al., 2016). In other scientific report Blasi et al.…”

“…Statistical analysis of the total phenols and flavonoids contents showed significant differences between the four cultivars, whilst the total chlorophyll amounts were not statistically different between cultivars. It can be hypothesized that the significant differences observed for total phenols and flavonoids may be related both to genetics and to the geographical origin (Kallithraka et al., 2004; Felhi et al., 2016).…”

RETRACTED: Antimicrobial and Wound Healing Potential of a New Chemotype from Piper cubeba L. Essential Oil and In Silico Study on S. aureus tyrosyl-tRNA Synthetase Protein

“…The antioxidants have been applied to remove the deleterious detrimental effects of ROS, by donating their own electrons, and therefore preventing them from capturing electrons from other important molecules, such as DNA, proteins and lipids [ 1 ]. Thus, various medicinal herbs and plants have been reported to offer promising biosources of bioactive molecules with various biological properties such as antioxidant [ 9 , 10 , 11 , 12 , 13 , 14 ], antimicrobial [ 10 , 12 , 13 , 14 , 15 ], antibiofilm [ 16 , 17 ], antiquorum sensing [ 11 , 17 , 18 ] and antidiabetic [ 19 ]. On the other hand, several treatments are available for the wound management such as analgesics, antibiotics and nonsteroidal anti-inflammatory drugs, but most of them produce numerous unwanted side effects [ 8 , 20 ].…”

Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits

“…Dn the other hand, we noted the presence of many major bands in the spectral range of 1600-600 cm -1 which reflect that some bioactive molecules of significance have been detected in all the extracts with nutraceutical effect and further indicate the presence of withanolides since these frequencies are characteristic of ether, carboxylic acids and esters (Gorinstein et al, 2010). Felhi et al (2016b) carried out the FT-IR spectral analysis of freeze-dried extract obtained from fruit juice of E. elaterium and reported the presence of characteristic functional groups such as ─DH, C─D, -C≡C and C─H of phenolic compounds, carboxylic acids, alcohols, carbohydrates, and proteins in the plant, that are responsible for various medicinal properties. The various functional groups observed in different extracts reflected the biochemical compositions of E. elaterium.…”