Pineapple Waste and Wastewater: Route for Biodiesel Production from Candida tropicalis (MF510172)

Abstract

The aim of the present study was to utilize waste resources for biodiesel production and to test its recyclability. The oleaginous yeast was isolated from local municipal wastewater outlet and on the basis of 18S r RNA sequencing, isolate was identified as Candida tropicalis (MF510172). Optimum fermentation conditions for lipid yield was recorded to be at 180 rpm agitation speed, 6 % pineapple waste as a carbon source, 5 % inoculum size,48 h inoculum age at 40°C temperature with 72 h incubation time. Candia was able to produce 13 ml. L-1 lipids using optimum conditions. Extracted lipids were transesterified to form fatty acid methyl esters (FAME) and further characterized by using GC-MS and FTIR spectroscopy. Palmitic acid methyl esters and other supplementary hydrocarbon compounds were identified using GC-MS. FTIR spectrum indicates the characteristics FAME peak at 1740 cm-1 with C= O stretching band of methyl esters. The results clearly demonstrate the waste resources containing filtered wastewater and pineapple waste used in the study are effective to produce quality oil for biodiesel production.

Valorization of agricultural wastes for biofuel applications

“…(2016) synthesized biodiesel from oleaginous microalgae grown on an extract from sugarcane bagasse and reported a biodiesel yield of 112 ± 5.2 mg/L. Other reported production of biodiesel from corn stover ( Le et al., 2017 ; Shafiei Alavijeh et al., 2020 ) cassava starch ( Zhang et al., 2022a ), pineapple waste ( Kanakdande et al., 2020 ), and rice bran oil ( Ibrahim et al., 2020 ) are shown in Table 8 .…”

Application of Pineapple Waste to the Removal of Toxic Contaminants: A Review

“…The findings showed a total solid content of 17–19%, a volatile solid content of 85–95%, and a pH range of 3.3–6.7%. While total biogas results revealed that pineapple peel had a higher value (571.4 mL) than banana peel (386.6 mL) [ 81 ]. In a similar study, Suphang et al [ 78 ] utilized pineapple peel solid waste to successfully generate methane gas at a concentration of 48% at 20 days.…”

Recent Updates on the Conversion of Pineapple Waste (Ananas comosus) to Value-Added Products, Future Perspectives and Challenges

“…The conversion of triglyceride to FAME requires the reaction with alcohol with the help of a catalyst to produce glycerin and FAME. An attempt to utilise pineapple waste to produce biodiesel was performed by Kanakdande et al [21]. Due to the high sugar content such as fructose, sucrose and glucose in pineapple waste, the authors used pineapple waste as the carbon source for lipid extraction in producing biodiesel.…”

“…Pectin, lignin, cellulose and their derivatives from pineapple waste are the major components needed in producing nanocellulose and green packaging [13][14][15]. Since pineapple waste is a good carbon source, organic acid [16], wine [17], vinegar [18], dye adsorbent [19], biofuel [20][21][22] and biogas [23][24][25] can be produced from it. The properties and characteristic of waste feedstock are crucial factors that need to be understood before the conversion.…”