The aim of the present study was to utilize waste resources for biodiesel production and to test its recyclability. The oleaginous yeast was isolated from local municipal wastewater outlet and on the basis of 18S r RNA sequencing, isolate was identified as Candida tropicalis (MF510172). Optimum fermentation conditions for lipid yield was recorded to be at 180 rpm agitation speed, 6 % pineapple waste as a carbon source, 5 % inoculum size,48 h inoculum age at 40°C temperature with 72 h incubation time. Candia was able to produce 13 ml. L-1 lipids using optimum conditions. Extracted lipids were transesterified to form fatty acid methyl esters (FAME) and further characterized by using GC-MS and FTIR spectroscopy. Palmitic acid methyl esters and other supplementary hydrocarbon compounds were identified using GC-MS. FTIR spectrum indicates the characteristics FAME peak at 1740 cm-1 with C= O stretching band of methyl esters. The results clearly demonstrate the waste resources containing filtered wastewater and pineapple waste used in the study are effective to produce quality oil for biodiesel production.
The continuous rising demands and fluctuations in the prices of fossil fuels warrant searching for an alternative renewable energy source to manage the energy needs.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.