In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential

Carvalho, Paula Pinheiro de; Antoniazzi, Camila Aparecida; Faria, Rodrigo Brito de; Carvalho, Ilio Fealho de; Rocha, Diego Ismael; Silva, Maurecilne Lemes da

doi:10.1590/1678-4324-2019170803

Cited by 6 publications

(8 citation statements)

References 32 publications

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“…MS medium supplemented with 1.0 mg/l TDZ was the best treatment, producing the highest percentage of organogenesis formation of 76% and the highest mean number of shoots/explant of 17. This result is in harmony with that obtained by De Carvalho et al [53] who found that TDZ-induced direct organogenesis from leaf explants of Passiflora miniata.…”

Section: Discussionsupporting

confidence: 92%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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Background Dimocarpus longan is a tropical tree that produces edible fruit. It is a neglected plant species that is listed as near threatened. In spite of its economic value, the propagation of longan cultivar using conventional methods is extremely difficult. The goal of this research is to produce and conserve this plant through in vitro propagation. Results In order to form new shoots, sterilized shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) or 2-isobentenyl-adenine (2ip). For direct organogenesis, young leaves of new shoots were cultured on MS medium fortified with various concentrations of Thidiazuron (TDZ) or 6-(4-Hydroxy-3-methylbut-2-enylamino purine) (Zeatin). Gibbrellic acid (GA3) at different levels alone or in combination was used for shoot elongation. Also, indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) were used for root formation. MS medium supplemented with 1.00 mg/l 2ip was suitable for inducing axillary shoots from shoot tips (4.0 axillary shoots/explant). The highest significant 76% and numbers of adventitious buds from leaf base were achieved on MS medium containing 1.0 mg/l TDZ. These buds developed into the longest plantlets on GA3 at 3.0 mg/l and rooted well in ½MS containing 1.50 mg/l IBA plus 0.50 mg/l (NAA). About 70% in vitro plants were successfully acclimatized. The AFLP profile illustrated the genetic stability of gene expression action. The amplified fragment length polymorphisms (AFLPs) profile illustrated the progenies were extremely similar to the mother plants. According to our findings, MS medium containing 25 ppm salicylic acid (SA) and 5 ppm methyl jasmonate (MeJA) produced the highest percentage of apigenin in longan calli (77.09 and 2.637%, w/w). Conclusion A successful and efficient micropropagation protocol has been developed and described here for the first time, and it will be very useful for the clonal propagation and conservation of the near-threatened Dimocarpus longan plant. Micropropagated plants are genetically identical to the donor plant using the AFLP technique. The usefulness of salicylic acid and methyl jasmonate as elicitors for increasing in vitro production of secondary metabolites in plants is demonstrated in this work.

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Section: Discussionsupporting

confidence: 92%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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“…It was reported that the maximum response on medium supplemented with 1.0 mg/l BA and 0.5 mg/l TDZ was observed, producing an average of 18.2 buds per culture after 24 weeks from date palm culture [ 19 ]. TDZ at 2.0 mg/l also could achieve shoot sprouting directly on leaf tissues of Urginea altissima [ 24 ], at 1.5 mg/l for Passiflora miniata [ 25 ] and at 1.0 mg/l for Aloe vera [ 26 ] and Arnebia euchroma [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars

Taha

Allam

Hassan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Inflorescence explants of date palm proved to be a promising tool for micropropagation of elite cultivars or rare males and females as organogenesis and somatic embryogenesis could be achieved. These plant materials are abundantly available every year and can be used as cheap and potent explants. Nevertheless, many difficulties could be faced in this protocol according to selection of the spathe size and age, media components, growth regulators, etc. The aim of this study was to determine the influence of various cytokinins on direct organs induction of three date palm cultivars (Selmi, Barhee, and Medjool) from immature inflorescence. An additional objective of this study was to investigate the effect of cytokinins and auxins on growth and development of Medjool cultivar. Results Various combinations of cytokinins were investigated on three date palm inflorescences as N6-(2-isopentenyl) adenine (2iP), kinetin, benzyleadenine (BA), and thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-yl urea) (TDZ). TDZ alone or in combination with BA proved to be superior for direct organogenesis in all three cultivars so that another combination of TDZ with BA was conducted. Results showed that moderate concentration of BA, with TDZ, gave superior response. Medjool cultivar response surpassed other two cultivars that made the possibility to conduct some growth regulators treatments on its multiplication and regeneration. TDZ at 0.5 + BA at 1.0 mg/l without activated charcoal seemed to enhance multiplication rate. Medium containing 0.5 mg/l of both naphthaleneacetic acid and indole butyric acid in addition to 1.0 mg/l indole acetic acid appeared to be more suitable for rooting stage of Medjool shootlets. Conclusion In this study, we created an innovation sequence of growth regulators included in nutrient media for date palm direct organogenesis from inflorescence. Organogenesis has been accelerated from immature inflorescence explants and developed to healthy plantlets which acclimatized in greenhouse.

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“…In all three cultivars, TDZ alone or in combination with BA was found to be superior for direct organogenesis, so a new TDZ-BA combination was tested. TDZ at 2.0 mg/l could induce shoot in Urginea altissima leaf tissues (Baskaran et al, 2018), 1.5 mg/l in Passiflora miniata (Carvalho et al, 2019), and 1.0 mg/l in Aloe vera leaf tissues (Lavakumaran and Seran, 2014). TDZ at 0.5 and 5.0 µM was found to be optimum for inducing an average of 4-5 shoots per cotyledonary node in 93% of the cultures and 55 somatic embryos in 68% of the cultures by Chhabra et al (2008).…”

Section: Discussionmentioning

confidence: 90%

Effect of Micropropagation Conditions on Adventitious Buds Formation and the Circadian Expression of the ACO013229.1 Gene in Ananas Comosus

Ahmed

El-Fadl

Suliman

et al. 2021

Egyptian Journal of Desert Research

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mooth cayenne pineapple cultivar is considered the most suitable variety for the climatic conditions in Egypt, in addition to its distinctive flavor and ability for canning. To meet market demand, large quantities of plant materials are required, which cannot be obtained via traditional breeding methods. As a result, an in vitro technique was designed to increase the multiplication rate, rooting, and acclimatization of this unique pineapple variety. Thidiazuron (TDZ) at 2.0 mg/l proved to be superior for direct organogenesis rate. Half strength Murashige and Skoog (MS) medium containing 1.0 mg/l indole-3-butyric acid (IBA) in combination with 0.5 mg/l naphthalene acetic acid (NAA) improved the number and length of roots. Organogenesis has been accelerated from in vitro derived leaves and developed to healthy plantlets, which were acclimatized in the greenhouse. In order to investigate the effect of micropropagation on circadian rhythm, the circadian expression of Aco013229.1 was compared, which belongs to the MADS-box gene family, between the in vitro propagated plantlets and the in vivo-grown plants. The unaffected expression pattern of Aco013229.1 proposed that in vitro micropropagation did not affect the circadian cycling; hence, the CAM photosynthesis process was not interrupted. Moreover, the circadian expression of Aco013229.1 of the in vitro and in vivo-grown plants showed a similar pattern, strongly pointing at a stable circadian rhythm of the micropropagated plants and thus a well-maintained CAM photosynthesis. This gene family plays a significant role in a number of biological processes especially flowering.

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In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential

Cited by 6 publications

References 32 publications

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars

Effect of Micropropagation Conditions on Adventitious Buds Formation and the Circadian Expression of the ACO013229.1 Gene in Ananas Comosus

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