Molecular detection of Mycoplasma gallisepticum in different poultry breeds of Abbottabad and Rawalpindi, Pakistan

Abstract

The poultry sector in Pakistan is contributing mainly in bridging gap between demand and supply for protein. Mycoplasma gallisepticum is an emerging bacterium causing serious problems in poultry industry of Pakistan. A cross-sectional study was conducted to evaluate the M. gallisepticum load in poultry populated regions of Pakistan. Total 600 serum and 600 swab samples were collected, 200 from each broiler, layers and breeders poultry in Rawalpindi and Abbottabad districts. Serum samples were analyzed through ELISA for seroprevalence. Swabs were cultured on Frey’s medium followed by PCR and partial mgc2 gene sequencing. Results of seroprevalence of M. gallisepticum showed that layers (75%, n=150) are more positive as compared to breeders (70%, n=140) and broilers (50%, n=100). Typical colonies of the M. gallisepticum were observed in breeder (26.5%), followed by layer (21%) and broilers (9%). A total of 37.1% (n=42) samples were identified positive through PCR out of total 113 cultured based positive samples. A total of six M. gallisepticum isolates of current study showed 98-99 percent similarity with previously reported isolates on the basis of mgc2 gene partial sequencing. The M. gallisepticum was found highly prevalent in different poultry breads. Results of this study would add into basic data and provide a direction for livestock sector to strengthen a control strategy for mycoplasmosis in poultry farms.

Seroprevalence of Mycoplasma gallisepticum in Commercial Broiler Chickens in Duhok Governorate

“…This program necessitates strong biosecurity and an efficient monitoring mechanism. Medication can assist to prevent clinical signs and lesions [28]. The eradication of organisms from breeding flocks and their progeny has been the mainstay of MG control, with periodic serological monitoring, followed by culture and/or PCR monitoring, confirming the flocks' Mycoplasma-free status [12,29,30].…”

Epidemiological and Diagnostic Status of Mycoplasma Synoviae in Pakistan and India: A Review

“…Over 110 species of genus Mycoplasma have been identified and isolated in many living organisms including birds, mammals, fish and reptiles (Elgnay et al 2013). Mycoplasmosis includes several pathogenic mycoplasma species including Mycoplasma synoviae (MS), Mycoplasma gallisepticum (MG), Mycoplasma meleagridis (MM) and Mycoplasma iowae (MI) are of utmost importance, in which MS and MG are of vital significance and known as notifiable pathogens by OIE (office of international and epizootics) (Stipkovits and Kempf 1996;Ishfaq et al 2020;Muhammad et al 2021). MS mostly spreads disease in chickens, but it is also reported in wild and domestic birds.…”

Lnc90386 Sponges miR-33-5p to Mediate Mycoplasma gallisepticum-Induced Inflammation and Apoptosis in Chickens via the JNK Pathway

“…Upon these processes, MG can induce inflammation damage and apoptosis by releasing genetic material and enzymes into the host cell (4,5). MG can exist for a long time and be difficult to be eliminated from infected birds, leading to serious economic losses in the poultry industry worldwide (6)(7)(8). Therefore, it is urgent to find a new and effective way to block the transmission of MG or treat MG-induced inflammatory damage and apoptosis (9)(10)(11).…”