Lnc90386 Sponges miR-33-5p to Mediate Mycoplasma gallisepticum-Induced Inflammation and Apoptosis in Chickens via the JNK Pathway

Abstract

Mycoplasma gallisepticum (MG) is one of the most important pathogens, that causes chronic respiratory disease (CRD) in chickens. Long non-coding RNAs (lncRNAs) are emerging as new regulators for many diseases and some lncRNAs can function as competing endogenous RNAs (ceRNAs) to regulate mRNAs by competitively binding to miRNAs. Here, we found that miR-33-5p was significantly up-regulated both in MG-infected chicken embryonic lungs and chicken embryo fibroblast cells (DF-1), and Lnc90386 negatively correlated with miR-33-5p. miR-33-5p, as a new regulator for MG infection, repressed apoptosis, inflammatory factors in DF-1 cells by targeting JNK1. Further analyses showed that Lnc90386 sponged miR-33-5p to weaken its inhibitory effect on JNK1, forming the ceRNA regulatory network. Furthermore, knockdown of Lnc90386 significantly inhibited apoptosis and inflammatory factors, and promoted DF-1 cells proliferation. However, co-treatment with miR-33-5p inhibitor and Lnc90386 siRNA showed that knockdown of Lnc90386 could partially eliminate the inhibiting effect of miR-33-5p inhibitor on inflammation, cell apoptosis and proliferation. In conclusion, Lnc90386 sponges miR-33-5p to defend against MG infection by inhibiting the JNK signaling pathway.

Exosomal microRNA/miRNA Dysregulation in Respiratory Diseases: From Mycoplasma-Induced Respiratory Disease to COVID-19 and Beyond

“…Specifically, we identified lnc90386 as a sponge for miR-33-5p, which weakens its inhibitory effect on JNK1. By doing so, lnc90386 defends against MG infection by inhibiting inflammation and apoptosis in DF-1 cells [21]. This finding highlights the importance of the lncRNA-mediated regulation of miRNA function in the context of MG infection.…”

“…Exosomal miRNA sequencing data revealed that 30 miRNAs were significantly differentially expressed in the MG-infected group compared to the non-infected group [9]. Several of these miRNAs were selected for further study of their functions [9,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], and this review section summarizes our research on the roles of miRNAs and exosomal miRNAs in regulating signaling pathways during MG infection in chickens (Figure 1, Table 1).…”

Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis

“…MG-HS, a virulent strain, was isolated previously from a chicken farm in Hubei Province of China [ 32 ]. The concentration of viable Mycoplasmas in a suspension was then determined by a color-changing unit (CCU) assay that was reported in detail in our previous studies [ 33 ]. The concentration of MG-HS in this study was 10 9 CCU/mL.…”

Immune Evasion of Mycoplasma gallisepticum: An Overview

“…In addition, some microRNAs promoted cell proliferation and attenuated the inflammatory response and apoptosis to resist M. gallisepticum infection. It was found that upon M. gallisepticum infection, Lnc90386 sponged miR-33-5p to negatively regulate the c-Jun N-terminal kinase (JNK) pathway and inhibited M. gallisepticum pMGA1.2 expression, promoting cell proliferation and inhibiting inflammatory damage and apoptosis caused by M. gallisepticum infection [ 98 ].…”