Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model

Ribeiro, Thuanne Pires; Sousa, T. R. de; Arruda, Andrelisse; Peixoto, Nei; Gonçalves, Pablo José; Almeida, Luciane Madureira

doi:10.1590/1519-6984.20114

Cited by 40 publications

(23 citation statements)

References 18 publications

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“…The fruit extract and the trunk latex exhibited anti-inflammatory activity in animal models [ 36 , 37 ]. Additionally, the latex showed significant angiogenic activity and a potential osteogenic effect, and had no cytotoxic or genotoxic effects on life systems [ 19 , 38 , 39 ]. Chemical analysis of H. speciosa latex resulted in the identification of naringenin-7- O -glucoside, chlorogenic acid, catechin, and procyanidin [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Bastos¹,

Dias²,

Nascimento³

et al. 2017

Molecules

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Apocynaceae is a botanical family distributed mainly in tropical and subtropical regions of the world. In Brazil, they comprise about 90 genera and 850 species, inhabiting various types of vegetation. Within this large botanical family, the genus Hancornia is considered monotypic, with its only species Hancornia speciosa Gomes. Antihypertensive, antidiabetic, and antiviral activities are described for this species. Despite having been the target of some studies, knowledge of its chemical composition is still limited. In this study, the phenolics of H. speciosa leaves were analyzed using ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high-resolution mass spectrometry (HRMS). As a result, 14 compounds were identified viz. protocatechuic acid, catechin, and quercetin, and another 14 were putatively identified viz. B- and C-type procyanidins, while just one compound remained unknown. From the identified compounds, 17 are reported for the first time viz. coumaroylquinic acid isomers and eriodyctiol. The results show that Hancornia speciosa can serve as source of valuable phenolics.

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Section: Discussionmentioning

confidence: 99%

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Bastos¹,

Dias²,

Nascimento³

et al. 2017

Molecules

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“…Here we selected two different methodologies to evaluate the V. polyanthes toxicological potential: Allium cepa and Artemia salina assays. Those methods are very useful for toxicity and cytogenotoxicity screening and they are simple, inexpensive and minimum laboratory facilities are required for its performance (Ribeiro et al, 2016;Neves et al, 2014;Leme and Marin-Morales, 2009). Moreover, the results obtained using those in vivo models show a high degree of conformity with the results obtained from mammalian assays (Freires et al, 2017;Fedel-Miyasato et al, 2014;Leme and Marin-Morales, 2009).…”

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confidence: 81%

Cytotoxic effect of Vernonanthura polyanthes leaves aqueous extracts

et al. 2021

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Vernonanthura polyanthes, popularly known as assa-peixe, is a medicinal plant that has been widely used by Brazilian Cerrado population for treatment of diseases without a detailed evaluation of their effectiveness, toxicity, and proper dosage. Thus, more studies investigating the safety of V. polyanthes aqueous extract before the use are needed. The purpose of this study was to evaluate the toxicity, cytotoxicity and genotoxicity of V. polyanthes leaves aqueous extract using the Artemia salina and Allium cepa assays. For the A. salina assay, three groups of 10 larvae were exposed to V. polyanthes leaves aqueous extract at the concentrations of 5, 10, 20, 40, and 80 mg/ml. For the A. cepa assay, 5 onion bulbs were exposed to V. polyanthes leaves aqueous extract at 10, 20, and 40 mg/ml, and then submitted to macroscopic and microscopic analysis. As result it was identified a toxicity and cytotoxicity of V. polyanthes dependent on the extract concentration. The A. salina assay suggests that the concentration of 24 mg/ml of the V. polyanthes extract is able to kill 50% of naupllis; while the A. cepa assay suggests that V. polyanthes leaves aqueous extract is toxic at concentrations higher than 20 mg/ml; however the cytotoxic effect in A. cepa root cells was observed at 40 mg/ml of the extract. It is important to say that the V. polyanthes leaves aqueous extract concentration commonly used in popular medicine is 20 mg/ml. Thus, the popular concentration used is very close to toxicity limit in A. salina model (24 mg/ml) and is the concentration which showed toxic effect in A. cepa root cells (20 mg/ml). No genotoxic activity of V. polyantes leaves aqueous extract was observed in the conditions used in this study. Because of the antiproliferative action and no genotoxic activity, V. polyanthes leaves aqueous extract may present compounds with potential use for human medicine. However more detailed studies need to be performed to confirm this potential.

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“…H. speciosa is a medicinal species traditionally used to treat a plethora of conditions, including skin diseases, tuberculosis, hypertension, and gastrointestinal problems [4,5]. Previously, our research group has demonstrated the chemopreventive [6], antihypertensive [7], antidiabetic [8], and wound healing [9] properties of the species.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

Silva

Pacheco

Madeira

et al. 2019

PMIO

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Supporting Information for this article is available online at http://www.thieme-connect.de/products. ABSTR AC THancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-( + )-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.

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Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model

Cited by 40 publications

References 18 publications

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Cytotoxic effect of Vernonanthura polyanthes leaves aqueous extracts

Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

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