Eggs viability of Aedes aegypti Linnaeus (Diptera, Culicidae) under different environmental and storage conditions in Manaus, Amazonas, Brazil

Abstract

The viability of Aedes aegypti eggs was assessed in the Amazon region. The eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. Egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. There were significant differences in the viability of Ae. aegypti eggs with respect to the location (F=30.40; DF=1; P<0.0001), storage type (F=17.66; DF=2; P<0.0001), and time of storage (F=49.56; DF=9; P<0.0001). The interaction between storage site versus storage type was also significant (F=15.96; DF=2; P<0.0001). A higher hatching mean was observed for the eggs kept in the insectarium than for those outdoors (32.38 versus 7.46). Hatching rates of egg batches stored for 12 to 61 days ranged between 84 and 90%. A reduction was observed between 89 and 118 days, with values of 63 and 48%, respectively. With respect to type of storage, mean egg hatching was higher for the eggs in plastic cups (44.46). It was concluded that the viability of the eggs of Ae. aegypti in the Amazon region remains high up to 4 months, after which it declines drastically, although in this study hatching occurred for up to 8 months in very low percentages.Keywords: oviposition, hatching rate, Aedes aegypti, density, Amazon. Viabilidade de ovos de Aedes aegypti (Diptera, Culicidae) em diferentes condições de armazenamento em Manaus, Amazonas, Brasil ResumoTestou-se a viabilidade dos ovos de Ae. aegypti na região amazônica armazenados em copos plásticos, envelopes de papel e sacos plásticos que foram mantidos em área interna e em área externa por dias diferentes. Verificaram-se diferenças significativas na viabilidade dos ovos considerando-se a localização (F=30,40; GL=1; P<0,0001), os tipos de armazenamento (F=17,66; GL=2; P<0,0001) e os tempos (F=49,56; GL=9; P<0,0001) e na interação localização versus tipo de armazenamento (F=15,96; GL=2; P<0,0001). A média de eclosão dos ovos armazenados no insetário foi maior: 32,38, do que a dos lotes armazenados na área externa: 7,46. As taxas de eclosão dos ovos armazenados de 12 a 61 dias ficaram entre 90% e 84% com reduções a partir de 89 e 118 dias, quando atingiu de 63% e 48%, respectivamente. Em relação aos tipos de armazenamento a média de ovos dos copos foi significativa (44,46). Conclui-se que a viabilidade dos ovos de Ae. aegypti na região amazônica é mantida em níveis elevados até 4 meses, e a partir daí ocorrem reduções drásticas, com eclosões até 8 meses em percentuais muito baixos.Palavras-chave: oviposição, taxa de eclosão, Aedes aegypti, densidade, Amazônia.

Quiescence in Aedes aegypti: Interpopulation Differences Contribute to Population Dynamics and Vectorial Capacity

“…However, important parameters for population dynamics and vectorial capacities such as fecundity, fertility, and mosquito size were not affected by quiescence. In agreement with previous studies on egg quiescence in A. aegypti indicating differential egg viability [ 12 , 17 , 21 , 22 , 45 , 46 , 47 ], in our study, the A. aegypti population from Recife proved to be more resistant to desiccation (130 days, with high egg viability until three months of quiescence), than mosquitoes from the Aracaju population (100 days, with a high hatching rate up to 40 days of quiescence). Even so, the overall quiescence was shorter compared with other similar studies in which maximum larvae hatching was recorded from 150 days up to more than a year [ 12 , 22 , 46 , 47 , 48 ].…”

Study of Aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries

“…aegypti eggs to enter diapause allowed their reproductive success. The diapause may extend for six months or more, until the eggs get in contact with water in the container again, and then hatching occurs 32 . In the cemeteries, it is not possible to control the rain factor, therefore, it is important to have a method to control the presence of larvae and pupae in the vases.…”

Assessing the re-introduction ofAedes aegyptiin Europe. Will the Yellow Fever Mosquito colonize the Old World?

“…The two shape parameters of this Beta distribution were derived using the average (0.076) and low 95% CI (0.023) proportion of Ae. aegypti eggs hatched after being submerged in plastic containers filled with water in field conditions (Soares-Pinheiro et al, 2016).…”