In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

Zimmermann, Carine Eloise Prestes; Machado, A. K.; Cadoná, F. C.; Jaques, Jas; Schlemmer, Karine Bizzi; Lautert, Claudia; Cruz, Ibm; Zanette, Régis A.; Leal, Daniela Bitencourt Rosa; Santurio, Jânio M.

doi:10.1590/1516-635x1603307-312

Cited by 14 publications

(6 citation statements)

References 28 publications

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“…; Zimmermann et al . ). In our study, we observed the apoptosis of PBHs treated with AFB1, which was consistent with the results from previous studies.…”

Section: Discussionmentioning

confidence: 97%

Aflatoxin B1 impairs mitochondrial functions, activates ROS generation, induces apoptosis and involves Nrf2 signal pathway in primary broiler hepatocytes

Liu

Wang

2016

Animal Science Journal

108

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Aflatoxin B1 (AFB1) is known as a mycotoxin that causes various health problems in animals, but the precise mechanism of AFB1 on mitochondrial functions and apoptosis in primary broiler hepatocytes (PBHs) is not clear. The objective of this study was to investigate the effects of AFB1 on the mitochondrial functions, reactive oxygen species (ROS) generation, apoptosis and nuclear factor erythroid 2-like factor 2 (Nrf2)-related signal pathway in PBHs. Here, the mitochondrial membrane potential (MMP), ROS generation, antioxidative genes and apoptosis in PBHs induced by AFB1 were investigated. The results showed that AFB1 evoked mitochondrial ROS generation, decreased MMP and induced apoptosis in PBHs. AFB1 increased the percentage of apoptotic cells, and expression of caspase-9 and caspase-3, upregulated messenger RNA (mRNA) expression of Nrf2 and downregulated mRNA expressions of NAD(P)H: quinine oxidoreductase 1, superoxide dismutase and Heme oxygenase 1 in PBHs. The expression of Bax was also observed in cytoplasm. These findings suggested AFB1 results in a significant impairment of mitochondrial functions, activates ROS generation, induces apoptosis, and is involved in Nrf2 signal pathway through mitochondria ROS-dependent signal pathways in PBHs.

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“…; Zimmermann et al . ). In our study, we observed the apoptosis of PBHs treated with AFB1, which was consistent with the results from previous studies.…”

Section: Discussionmentioning

confidence: 97%

Aflatoxin B1 impairs mitochondrial functions, activates ROS generation, induces apoptosis and involves Nrf2 signal pathway in primary broiler hepatocytes

Liu

Wang

2016

Animal Science Journal

108

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“…Chronic AFB1 intake has been shown to result in poor health, a loss in productivity, as well as immune system dysfunction. This appears to be due to the damage to cell DNA (Zimmermann et al, 2014). β-Glucans from the yeast Saccharomyces cerevisiae have been shown in vitro to be cytoprotective and genoprotective of chicken lymphocytes (Zimmermann et al, 2015).…”

Section: Glucansmentioning

confidence: 99%

Glucans and the Poultry Immune System

Jacob¹,

Pescatore²

2017

American Journal of Immunology

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Abstract:With the reduced availability of antibiotics, poultry producers are looking for feed additives to stimulate the immune system of their birds. Some beta-glucans have been shown to improve gut health in poultry subjected to a bacterial challenge, to increase the flow of new immunocytes into the various lymphoid organs, to increase macrophage function, to increase antibody titers after a vaccination and to function as an anti-inflammatory immunomodulator. As a result, beta-glucans may provide a tool for producers trying to reduce or eliminate the use of antibiotics in poultry diets.

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“…In this regard, more investigations are required to explain the mechanism of phenolic fractions influences when it was present in fungal growth media. Where aflatoxin is considered a carcinogenic chemical compound produced by toxigenic fungi, it was reported to decrease the viability of healthy cell-line [47,48].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Bottle Gourd Seeds’ Extracts in Chemical Hazard Reduction Secreted as Toxigenic Fungi Metabolites

Abdel–Razek

Badr

Alharthi

et al. 2021

Toxins

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Bottle gourd seeds are surrounded by innumerable bioactive components of phytochemicals. This work aimed to evaluate the effectiveness of bottle gourd extracts as antimicrobial and an-ti-mycotoxigenic against toxigenic fungi and mycotoxins. Polar and nonpolar extracts were made from the seeds. The polar eco-friendly extract was prepared by an ultrasonication-assisted technique utilizing aqueous isopropanol (80%), whereas the non-polar extract was obtained using petroleum ether (40–60). The antioxidant efficacy, total phenolic content, and flavonoid content of the extracts were all measured. The fatty acid profile was measured using GC equipment, and the influence on toxigenic fungus and mycotoxin release was also investigated. The antioxidant efficacy of the polar extract is reflected. The total phenolic values of the oil and polar extract were 15.5 and 267 mg of GAE/g, respectively. The total flavonoid content of the oil was 2.95 mg catechol/g, whereas the isopropyl extract of seeds contained 14.86 mg catechol/g. The polar extract inhibited the DPPH more effectively than oil. When compared to other seed oils, the fatty acid composition differed. The pathogens were distinguished by the MIC and MFC for the polar extract. Three sterols were found in the oil, with a high concentration of B-sitosterols. The oil’s valuable -carotene content and tocopherol content were recorded. When compared to traditional antibiotics, the polar extract has shown promising antimicrobial activity against infections and toxigenic fungi. Bottle gourd extracts, as a non-traditional bioactive source, are viewed as a potentially promising alternative that might contribute to increased food safety, shelf-life, and security.

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In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

Cited by 14 publications

References 28 publications

Aflatoxin B1 impairs mitochondrial functions, activates ROS generation, induces apoptosis and involves Nrf2 signal pathway in primary broiler hepatocytes

Aflatoxin B1 impairs mitochondrial functions, activates ROS generation, induces apoptosis and involves Nrf2 signal pathway in primary broiler hepatocytes

Glucans and the Poultry Immune System

Efficacy of Bottle Gourd Seeds’ Extracts in Chemical Hazard Reduction Secreted as Toxigenic Fungi Metabolites

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