Studying neurodegenerative diseases in culture models

Schlachetzki, J; Saliba, Soraya Wilke; Oliveira, Antônio Carlos Pinheiro de

doi:10.1590/1516-4446-2013-1159

Cited by 73 publications

(64 citation statements)

References 158 publications

(159 reference statements)

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“…Currently there are around eleven distinct morphologies of astrocytes identified, of these distinct subtypes eight are associated with the cerebral vasculature (Abbott, 2002). Astrocytes (or astroglia) are also the greatest in number and perform a myriad of functions including BBB homeostasis (Hawkins and Davis, 2005), volume regulation (Iacovetta et al, 2012), neuronal metabolism assistance through lactate shuttling (Schurr et al, 1997), neurotransmitter and ion buffering (Wong et al, 2013), disease (Schlachetzki et al, 2013; Sturrock, 1976), and secretion of basal lamina (Liesi et al, 1983). Astrocytes have also been shown to engage in slow-wave calcium signaling, and are hypothesized to contribute to the electrical behavior of neurons (Carlsen and Perrier, 2014).…”

Section: Use Of Cells In the In Vitro Modeling Of The Cnsmentioning

confidence: 99%

3D in vitro modeling of the central nervous system

Hopkins

DeSimone

Chwalek

et al. 2015

Progress in Neurobiology

194

188

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There are currently more than 600 diseases characterized as affecting the central nervous system (CNS) which inflict neural damage. Unfortunately, few of these conditions have effective treatments available. Although significant efforts have been put into developing new therapeutics, drugs which were promising in the developmental phase have high attrition rates in late stage clinical trials. These failures could be circumvented if current 2D in vitro and in vivo models were improved. 3D, tissue-engineered in vitro systems can address this need and enhance clinical translation through two approaches: (1) bottom-up, and (2) top-down (developmental/regenerative) strategies to reproduce the structure and function of human tissues. Critical challenges remain including biomaterials capable of matching the mechanical properties and extracellular matrix (ECM) composition of neural tissues, compartmentalized scaffolds that support heterogeneous tissue architectures reflective of brain organization and structure, and robust functional assays for in vitro tissue validation. The unique design parameters defined by the complex physiology of the CNS for construction and validation of 3D in vitro neural systems are reviewed here.

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Section: Use Of Cells In the In Vitro Modeling Of The Cnsmentioning

confidence: 99%

3D in vitro modeling of the central nervous system

Hopkins

DeSimone

Chwalek

et al. 2015

Progress in Neurobiology

194

188

View full text Add to dashboard Cite

show abstract

“…87 The presence of astrocytes and microglia in culture give these cultures the ability to better model certain diseases, especially diseases where inflammation is involved as an important disease modifying factor, considering that microglia and astrocytes are involved in secretion of inflammatory mediating factors. 88 Microglia in particular play an important role in injury and recovery, as shown when activated microglia mediate damage to injured dopaminergic cells, showing how inflammatory reactions could specifically target oxidative injuries. 89 As well as being cultured together as a mixture, neurons and glia can also be co-cultured in a segregated manner, with neurons and glia actively signalling each other while not being in contact.…”

Section: Co-culture Modelsmentioning

confidence: 99%

Tissue engineered organoids for neural network modelling

Kose-Dunn¹,

Fricker²,

Roach³

2017

ATROA

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The increased prevalence of neurological diseases across the world has stimulated a great deal of research into the physiological and pathological brain, both at clinical and pre-clinical level. This has led to the development of many sophisticated tissue engineered neural models, presenting greater cellular complexity to better mimic the central nervous system niche environment. These have been developed with the ambition to improve pre-clinical assessment of pharma and cellular therapies, as well as better understand this tissue type and its function/dysfunction. This review covers the necessary considerations in in vitro model design, along with recent advances in 2Dculture systems, to 3D organoids and bio-artificial organs.

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“…In vitro models provide important insights into the pathogenesis of neurodegenerative disorders and represent an interesting approach for the screening of potential pharmacological agents (35, 36). To obtain scientifically valid research, experimental conditions must be strictly controlled: this often involves manipulating one single variable at a time while keeping the others constant, and then observing the consequences of that single specific change.…”

Section: In Vitro Tool For Neurodegenerative Studiesmentioning

confidence: 99%

Bovine Brain: An in vitro Translational Model in Developmental Neuroscience and Neurodegenerative Research

Peruffo

Cozzi

2014

Front. Pediatr.

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Animal models provide convenient and clinically relevant tools in the research on neurodegenerative diseases. Studies on developmental disorders extensively rely on the use of laboratory rodents. The present mini-review proposes an alternative translational model based on the use of fetal bovine brain tissue. The bovine (Bos taurus) possesses a large and highly gyrencephalic brain and the long gestation period (41 weeks) is comparable to human pregnancy (38–40 weeks). Primary cultures obtained from fetal bovine brain constitute a validated in vitro model that allows examinations of neurons and/or glial cells under controlled and reproducible conditions. Physiological processes can be also studied on cultured bovine neural cells incubated with specific substrates or by electrically coupled electrolyte-oxide-semiconductor capacitors that permit direct recording from neuronal cells. Bovine neural cells and specific in vitro cell culture could be an alternative in comparative neuroscience and in neurodegenerative research, useful for studying development of normal and altered circuitry in a long gestation mammalian species. Use of bovine tissues would promote a substantial reduction in the use of laboratory animals.

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Studying neurodegenerative diseases in culture models

Cited by 73 publications

References 158 publications

3D in vitro modeling of the central nervous system

3D in vitro modeling of the central nervous system

Tissue engineered organoids for neural network modelling

Bovine Brain: An in vitro Translational Model in Developmental Neuroscience and Neurodegenerative Research

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