Protective effects of sodium ferulate on flap transplantation via anti-inflammatory modulation and oxidative stress inhibition

Sun, Yong; Gao, Yu; Xu, Lingfeng; Zhang, Yifan; Cheng, Chen; Wei, Ke; Lin, Jian; Chen, G.; Liu, Caiyue; Li, Q.F.

doi:10.1590/1414-431x2020e10520

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…However, this effect did not change much with increasing the dose of Saffron in our experiment, and in both groups of Saffron 40 and Saffron 80, the rate of MPO activity decrease, was the same. Other studies [ 1 , 49 , 54 ] confirm our results about increasing the survival of skin flaps following a decrease in MPO activity.…”

Section: Discussionsupporting

confidence: 89%

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

et al. 2022

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Background Despite the improvements to enhance skin flap viability, the effects of ischemia-reperfusion (IR), oxidative stress, necrosis, and apoptosis are still challenging. Crocus sativus L. (Saffron) is highly noticeable due to its tissue-protective and antioxidant properties. So, we aimed to investigate its effects on skin flap viability, oxidative stress, apoptosis markers, histopathological changes, and mTOR/p-mTOR expression. Materials and Methods 40 Sprauge-Dawley rats, weighting 200–240 g, were divided into four groups including: (1) Sham (8 × 3 cm skin cut, without elevation); (2) Flap Surgery (8 × 3 cm skin flap with elevation from its bed); (3) Saffron 40 mg/kg + Flap Surgery; and (4) Saffron 80 mg/kg + Flap Surgery. Saffron was administrated orally for 7 days. At day 7, flap necrosis percentage, histopathological changes, malondialdehyde level, Myeloperoxidase and superoxide dismutase activity, Bax, Bcl-2, mTOR, and p-mTOR expression were measured. Protein expressions were controlled by β -Actin. Results Saffron administration decreased flap necrosis percentage ( p < 0.01), which was not dose-dependent. Treatment groups showed significant histological healing signs (Neovascularization, Fibroblast migration, Epithelialization, and Epithelialization thickness), decreased MDA content ( p < 0.01), increased SOD ( p < 0.01) and decreased MPO activity ( p < 0.01). Bax and Bcl-2 expression, decreased and increased respectively in treated groups ( p < 0.0001). mTOR and p-mTOR expression were not changed significantly in Saffron treated groups. Conclusion Saffron could increase skin flap viability, alleviate necrosis, decrease oxidative stress and decrease apoptotic cell death, after skin flap surgery, but it acts independent of the mTOR pathway. So, Saffron could potentially be used clinically to enhance skin flap viability. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. https://www.springer.com/00266 Supplementary Information The online version contains supplementary material available at 10.1007/s00266-022-03048-6.

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Section: Discussionsupporting

confidence: 89%

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

et al. 2022

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“…When the blood flow of ischemic tissue is restored in the recovery process of the flap injury, oxygen molecules brought by blood can easily react with the cell membrane to perform and also destroy lipid peroxidation, resulting in cell death and tissue necrosis. Therefore, oxidative stress is a key determinant of tissue survival (Zhou et al, 2019;Sun et al, 2021). TBHP can stably cause oxidative stress in endothelial cells (Lin et al, 2019); thus, it was used as an in vitro stimulus to simulate the oxidative stress process in the survival of the skin flaps.…”

Section: Introductionmentioning

confidence: 99%