Upregulation of microRNA 142-3p in the peripheral blood and urinary cells of kidney transplant recipients with post-transplant graft dysfunction

Domenico, Tuany Di; Joelsons, Gabriel; Montenegro, Rosangela Munhoz; Manfro, Roberto Ceratti

doi:10.1590/1414-431x20175533

Cited by 13 publications

(5 citation statements)

References 39 publications

(47 reference statements)

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“…In summary, further studies with other potential biomarkers of IRI and acute rejection, perhaps involving already surveyed microRNAs such as miR-146a-5p and miR-142-3 in different non-invasive samples might contribute to the development of accurate non-invasive biomarkers(s) for use in clinical organ transplantation40.…”

Section: Discussionmentioning

confidence: 99%

Micro RNA 146a-5p expression in Kidney transplant recipients with delayed graft function

et al. 2019

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Introduction: The development of novel non-invasive biomarkers of kidney graft dysfunction, especially in the course of the delayed graft function period would be an important step forward in the clinical practice of kidney transplantation. Methods: We evaluated by RT-PCR the expression of miRNA-146 to -5p ribonucleic micro-acids (miRNAs) in the peripheral blood and renal tissue obtained from kidney transplant recipients who underwent a surveillance graft biopsy during the period of delayed graft function. Results: In biopsy samples, the expression of miR-146a-5p was significantly increased in the group of patients with delayed graft function (DGF) (n = 33) versus stables patients (STA) (n = 13) and patients with acute rejection (AR) (n = 9) (p = 0.008). In peripheral blood samples, a non-significant increase of miR-146a-5p expression was found in the DGF group versus STA and AR groups (p = 0.083). No significant correlation was found between levels of expression in biopsy and plasma. ROC curve analysis revealed an AUC of 0.75 (95% CI: 0.62-0.88) for the renal tissue expression and 0.67 (95% CI 0.52-0.81) for the peripheral blood expression. Conclusion: We conclude that miR-146a-5p expression has a distinct pattern in the renal tissue and perhaps in the peripheral blood in the setting of DGF. Further refinements and strategies for studies should be developed in the field of non-invasive molecular diagnosis of kidney graft dysfunction.

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Section: Discussionmentioning

confidence: 99%

Micro RNA 146a-5p expression in Kidney transplant recipients with delayed graft function

et al. 2019

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“…Barabadi [30] AR (27) ALL-B (normal, 45; CAN, 19) FOXP3 mRNA expression was significantly higher in AR (p < 0.001) Mockler [31] * TCMR (5; borderline, 3) STA-B There was no significant association between 6 months post-transplant CCL2 and TCMR changes (p = 0.46) Gandolfini [36] TCMR (22) ALL-B (normal, 19) CXCL9 > 200 pg/mL in TCMR, 100-200 in dysfunction graft, and < 100 pg/mL in stable graft (p < 0.01) Domenico [38] AR 23ALL-B (ATN, 18; normal, 8) mirRNA 142-3p was significantly higher in AR compared to stable graft (p < 0.001); not compared to ATN (p = 0.079) Lee [39] AR (8) STA (8); DYS-B (ATN, 8; other, 4)…”

Section: Ref Outcome (N) Control Group (N) Biomarkers Thresholds Anmentioning

confidence: 98%

“…Direct RT-PCR was used to identify targeted RNAs like programmed cell death protein1 (PD1) mRNA, forkhead box P3 (FOXP3) mRNA, and micro (mi) RNAs (miR-142-3p, miR-155-5p) [30,34,38,48], while genome-wide or transcriptome analysis were applied for the unbiased identification of circular and long noncoding RNAs [23,49].…”

Section: Scores and Formulasmentioning

confidence: 99%

Urinary Biomarkers for Diagnosis and Prediction of Acute Kidney Allograft Rejection: A Systematic Review

Guzzi

Cirillo

Buti

et al. 2020

IJMS

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Noninvasive tools for diagnosis or prediction of acute kidney allograft rejection have been extensively investigated in recent years. Biochemical and molecular analyses of blood and urine provide a liquid biopsy that could offer new possibilities for rejection prevention, monitoring, and therefore, treatment. Nevertheless, these tools are not yet available for routine use in clinical practice. In this systematic review, MEDLINE was searched for articles assessing urinary biomarkers for diagnosis or prediction of kidney allograft acute rejection published in the last five years (from 1 January 2015 to 31 May 2020). This review follows the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines. Articles providing targeted or unbiased urine sample analysis for the diagnosis or prediction of both acute cellular and antibody-mediated kidney allograft rejection were included, analyzed, and graded for methodological quality with a particular focus on study design and diagnostic test accuracy measures. Urinary C-X-C motif chemokine ligands were the most promising and frequently studied biomarkers. The combination of precise diagnostic reference in training sets with accurate validation in real-life cohorts provided the most relevant results and exciting groundwork for future studies.

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“…Urinary overexpression of miR-142-3p, associated with CKD [ 125 ], has also been found in 41 kidney transplant recipients (23 patients with acute rejection and 18 with acute tubular necrosis when compared to eight stable patients (p < 0.001 and p < 0.005, respectively) [ 151 ]. Peripheral blood analysis in these patients also showed overexpression of miR-142-3p as a marker of acute tubular necrosis (p < 0.05 for both acute tubular necrosis/stable and acute tubular necrosis/acute rejection comparisons) [ 151 ].…”

Section: Epigenetic Biomarkersmentioning

confidence: 99%

Genomic approaches in the search for molecular biomarkers in chronic kidney disease

et al. 2018

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BackgroundChronic kidney disease (CKD) is recognised as a global public health problem, more prevalent in older persons and associated with multiple co-morbidities. Diabetes mellitus and hypertension are common aetiologies for CKD, but IgA glomerulonephritis, membranous glomerulonephritis, lupus nephritis and autosomal dominant polycystic kidney disease are also common causes of CKD.Main bodyConventional biomarkers for CKD involving the use of estimated glomerular filtration rate (eGFR) derived from four variables (serum creatinine, age, gender and ethnicity) are recommended by clinical guidelines for the evaluation, classification, and stratification of CKD. However, these clinical biomarkers present some limitations, especially for early stages of CKD, elderly individuals, extreme body mass index values (serum creatinine), or are influenced by inflammation, steroid treatment and thyroid dysfunction (serum cystatin C). There is therefore a need to identify additional non-invasive biomarkers that are useful in clinical practice to help improve CKD diagnosis, inform prognosis and guide therapeutic management.ConclusionCKD is a multifactorial disease with associated genetic and environmental risk factors. Hence, many studies have employed genetic, epigenetic and transcriptomic approaches to identify biomarkers for kidney disease. In this review, we have summarised the most important studies in humans investigating genomic biomarkers for CKD in the last decade. Several genes, including UMOD, SHROOM3 and ELMO1 have been strongly associated with renal diseases, and some of their traits, such as eGFR and serum creatinine. The role of epigenetic and transcriptomic biomarkers in CKD and related diseases is still unclear. The combination of multiple biomarkers into classifiers, including genomic, and/or epigenomic, may give a more complete picture of kidney diseases.

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Upregulation of microRNA 142-3p in the peripheral blood and urinary cells of kidney transplant recipients with post-transplant graft dysfunction

Cited by 13 publications

References 39 publications

Micro RNA 146a-5p expression in Kidney transplant recipients with delayed graft function

Micro RNA 146a-5p expression in Kidney transplant recipients with delayed graft function

Urinary Biomarkers for Diagnosis and Prediction of Acute Kidney Allograft Rejection: A Systematic Review

Genomic approaches in the search for molecular biomarkers in chronic kidney disease

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