Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants

Abstract

Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.

Modulation of expression of proinflammatory genes and humoral immune response following immunization or infection with Aeromonas hydrophila in silver catfish (Rhamdia quelen)

“…In addition, by using adjuvants that differently modulate the maturation of Th cells, these vaccines might induce both humoral and cellular-mediated immunity [14] , [15] , [16] . The humoral immune response of silver catfish to inoculated bacteria [17] or purified antigens mixed with different types of adjuvant, including β-glucan, Montanide, Freund's complete and incomplete adjuvant, ODNs CpGs [18] , and inactivated Parapoxvirus ovis [39] has also been investigated; antigen-specific antibodies peak from 3 to 4 weeks post vaccination. However, there is no data regarding the early events following vaccination and protection to challenge with A. hydrophila .…”

Macrophage‐inducible C‐type lectin (MINCLE): In silico characterization and its in vitro effects on head–kidney leukocytes from the fishLutjanus peru

“…β‐glucans are polymers of glucose molecules linked by 1, 3/1, 6‐β‐D‐glycosidic bonds, which have been used as fish immunostimulants (Suchecka et al, 2015; Borchani et al, 2016; Pavan et al, 2016) and vaccine adjuvants (Rodríguez et al, 2016; Sanchez et al, 2021). In this regard, the combination or conjugation of antigenic proteins with β‐glucans is an innovative approach to vaccine development (Reyes‐Becerril et al, 2016; Reyes‐Becerril et al, 2020; Rubio‐Infante & Moreno‐Fierros, 2016).…”

“…β-glucans are polymers of glucose molecules linked by 1, 3/1, 6β-D-glycosidic bonds, which have been used as fish immunostimulants (Suchecka et al, 2015;Borchani et al, 2016;Pavan et al, 2016) and vaccine adjuvants (Rodríguez et al, 2016;Sanchez et al, 2021).…”