Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil

Santos, Ana Maria Ribeiro dos; Zidko, Ana Carolina Matos; Pignatari, Antônio Carlos Campos; Silva, Rosa Maria

doi:10.1590/1414-431x20133184

Cited by 30 publications

(31 citation statements)

References 20 publications

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“…The following standard strains were used as positive controls: J96 ( papA and pap C), A30 ( afaBC ), RS218 ( sfaDE , kpsMTII ) and SA101 ( iucD ) (Santos et al . ). The E. coli J53 strain was used as negative control in all the PCR reactions.…”

Section: Methodsmentioning

confidence: 97%

“…Genes associated with colonization factors (papA/papC, afaBC and sfaDE), siderophores (iucD) and serum resistance (kpsMTII) were investigated. The following standard strains were used as positive controls: J96 (papA and papC), A30 (afaBC), RS218 (sfaDE, kpsMTII) and SA101 (iucD) (Santos et al 2013). The E. coli J53 strain was used as negative control in all the PCR reactions.…”

Section: Molecular Characterization Of Genes Associated With Virulencementioning

confidence: 99%

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Putative virulence factors of extra‐intestinalEscherichia coliisolated from bovine mastitis with different clinical scores

Guerra

Dalanezi

Paula

et al. 2019

Lett Appl Microbiol

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We investigated the genes kpsMTII, iucD, sfaDE, afaBC, papA and papC, (proposed to be involved in extra‐intestinal pathogenic Escherichia coli—ExPEC), phylogroup classification and the in vitro swimming and swarming motility in 50 E. coli isolated from bovine mastitis with different clinical severity scores (mild, moderate and severe). The aforementioned genes were detected in 12 (n = 12/50; 24·0%) isolates. kpsMTII and iucD were the most frequent genes identified in six (n = 6/50; 12·0%) and four (n = 4/50; 8·0%) of the isolates, respectively. In only one (n = 1/50; 2·0%) isolate, more than one gene was simultaneously identified: iucD and kpsMTll were detected whereas sfaDE and afaBC were not detected. Mild, moderate and severe clinical signs were observed in 40·0% (n = 20/50), 28·0% (n = 14/50) and 32·0% (n = 16/50) of the cases. Commensal phylogroups B1 (n = 19/50; 38·0%) and A (n = 19/50; 38·0%) were prevalent; whereas pathogenic phylogroups B2 and D were observed in only 10·0% (n = 5/50). Swarming and swimming motilities were observed in 90·0% (n = 45/50) and 68·0% (n = 34/50) of the isolates, respectively; and there was a significant association (P = 0·0036) between swarming motility and severe clinical cases (score 3). To the best of our knowledge, this is the first study where clinical severity of bovine mastitis cases and the genes proposed to classify ExPEC were assessed in relation to swarming and swimming motility. Significance and Impact of the Study Escherichia coli is classified as extra‐intestinal (ExPEC) when strains contain at least two of the genes kpsMTII, iucD, sfaDE, afaBC and papA and/or papC. We investigated in vitro motility and the presence of these genes in 50 E. coli isolated from bovine mastitis with different clinical scores (mild, moderate and severe). Clinical severity was not associated with the genes studied. Swarming motility was associated with severe cases (score 3) of clinical mastitis. Results of this study contribute to a better understanding of the factors that determine the severity of clinical mastitis.

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Section: Methodsmentioning

confidence: 97%

Section: Molecular Characterization Of Genes Associated With Virulencementioning

confidence: 99%

Putative virulence factors of extra‐intestinalEscherichia coliisolated from bovine mastitis with different clinical scores

Guerra

Dalanezi

Paula

et al. 2019

Lett Appl Microbiol

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“…A high rate of quinolone resistance among bacteraemic E. coli strains (51%) and especially among E. coli ST131 strains (98%) limits the use of quionolones in clinical practice. The E. coli ST131 H30-Rx sublineage has emerged in the past 10 years and this sublineage is strongly associated with sepsis (Price et al, 2013;Santos et al, 2013). In this study, the overall prevalence of ST131 among the bloodstream isolates from the past 4 years was found to be 16% with an upward trend.…”

Section: Discussionmentioning

confidence: 59%

Molecular epidemiology of bloodstream-associated Escherichia coli ST131 H30-Rx subclone infection in a region with high quinolone resistance

Can

Azap

Nurtop

et al. 2016

Journal of Medical Microbiology

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Bloodstream infections caused by Escherichia coli ST131 and ST131 H30-Rx subclones have emerged worldwide. This study was carried out to evaluate the prevalence of the ST131-Rx subclone and characterize the virulence properties of the Rx isolates among the bloodstream E. coli isolates. A total of 297 non-duplicated E. coli bloodstream isolates were studied. Antibiotic susceptibilities were tested using the disc diffusion method. PCR amplification and sequencing was used to identify ST131 and H30-Rx, the virulence gene, the b-lactamase and virotype. Quinolone resistance among bacteraemic E. coli strains was 51 %, and it was 98 % among E. coli ST131 isolates. The ST131 isolates accounted for 16 % (49) of all isolates and all ST131 isolates belonged to the extraintestinal pathogenic E. coli. The proportion of H30 subclone among the ST131 isolates was 98 %, and 75 % of H30 isolates belonged to the H30-Rx subclone. The prevalence of ST131 increased from 13 to 23 % in 4 years; however, there was a decrease in the ratio of H30-Rx infections. CTX-M-15 was detected in 85 % of ST131 and all of the H30-Rx isolates. The virulence genes associated with adhesion, cell protection, iron uptake and toxins ( papA, iha, kpsMTII, iut and sat) were more common in ST131 than in non-ST131 isolates. Most of the ST131 and H30-Rx isolates were of the C virotype. All papA-positive isolates were in virotype C. The E. coli ST131 clone has increased rapidly among bloodstream isolates. However, a decrease in the proportion of the H30-Rx subclone in the quinolone-resistant population suggests the possibility of dissemination of other virulent and quinolone-resistant subclones in hospital settings. INTRODUCTIONEscherichia coli is one of the major causative agents of bloodstream infections (BSI). Management of BSIs caused by E. coli has become complicated due to the emergence of antimicrobial resistance and highly virulent clones Peirano & Pitout, 2014;Rogers et al., 2011). Several studies in the past two decades have reported antimicrobial resistance of E. coli strains against quinolones, cephalosporins and carbapenems (Dhanji et al., 2010;Ló pez-Cerero et al., 2014;Olesen et al., 2013;Wu et al., 2014).Extraintestinal pathogenic E. coli (ExPEC) are strains that carry special virulence factors and usually cause A detailed subST analysis of ST131 isolates is important to understand the ongoing emergence of fluoroquinoloneresistant E. coli. However, there is limited information available on the global distribution, virotypes and METHODSA total of 297 non-duplicated consequential E. coli strains isolated from patients with bloodstream infections at the Baskent University Hospital, Ankara, during a three-year period from 1 January 2010 to 1 July 2013 were included in this study. Susceptibility testing was performed using the disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (M2-A11) (CLSI, 2010). ESBL production was determined based on the CLSI standards (CLSI, 2010).PCR amplification and sequenci...

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“…Estos resultados difieren de los datos reportados en diversos países, donde el filogrupo B2 se ha reportado como el más prevalente en infecciones asociadas a la atención en salud (34)(35)(36)(37)(38)(39).…”

Section: Discussionunclassified

Genetic diversity of extraintestinal Escherichia coli strains producers of β-lactamases TEM, SHV and CTX-M associated with health-care

Varela¹,

Millán²,

Araque³

2017

biomedica

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Millán: caracterización fenotípica y molecular de las cepas estudiadas, análisis bioinformático María Araque: diseño del estudio y discusión crítica Todas las autoras participaron en la escritura del manuscrito. Introducción. En Venezuela existen pocos reportes que describan las bases genéticas del potencial patogénico y filogenético de las cepas de Escherichia coli provenientes de hospitales. Objetivo. Determinar la diversidad genética de cepas extraintestinales de E. coli productoras de las betalactamasas TEM, SHV y CTX-M asociadas a la atención de salud. Materiales y métodos. Se estudió una colección de 12 cepas extraintestinales de E. coli con sensibilidad disminuida a las cefalosporinas de amplio espectro. La sensibilidad antimicrobiana se determinó por concentración inhibitoria mínima. La detección de los grupos filogenéticos, de los factores de virulencia y de los genes que codifican la resistencia antimicrobiana se hizo mediante la técnica de reacción en cadena de la polimerasa y la relación clonal se estableció mediante reacción en cadena de la polimerasa de elementos palindrómicos extragénicos repetitivos (Repetitive Element Palindromic-PCR, rep-PCR). Diversidad genética de cepas extraintestinales deResultados. Todas las cepas analizadas presentaron resistencia a las cefalosporinas, y resistencia conjunta a quinolonas y aminoglucósidos. La distribución filogenética evidenció que los grupos A y B1 fueron los más frecuentes, seguidos por D y B2; en este último, se detectaron todos los factores de virulencia evaluados, y el gen más frecuente fue el fimH. En todas las cepas analizadas, se encontró bla CTX-M , con predominio de las bla CTX-M-8 , y en dos de estas cepas se evidenció la presencia simultánea de bla CTX-M-9 , variantes bla CTX-M-65 y bla CTX-M-147 . Conclusión. Las cepas estudiadas demostraron diversidad genética y albergaron diferentes genes de virulencia y betalactamasas de espectro extendido (BLEE) sin predominio de ningún filogrupo en particular. Este estudio constituye el primer reporte de la variante bla CTX-M-65 en Venezuela y de la variante bla CTX-M-147 en el mundo, en cepas no relacionadas genéticamente aisladas de hospitales, situación que merece atención y la racionalización del uso de los antimicrobianos.Palabras clave: Escherichia coli; infección hospitalaria; betalactamasas; filogenia; reacción en cadena de la polimerasa.doi: http://dx.doi.org/10.7705/biomedica.v37i3.3324 Genetic diversity of extraintestinal Escherichia coli strains producers of beta-lactamases TEM, SHV and CTX-M associated with healthcareIntroduction: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. Objective: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. Materials and methods:We studied a collection of 12 strains of extraintestinal E. coli with diminished sensitivity ...

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Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil

Cited by 30 publications

References 20 publications

Putative virulence factors of extra‐intestinalEscherichia coliisolated from bovine mastitis with different clinical scores

Putative virulence factors of extra‐intestinalEscherichia coliisolated from bovine mastitis with different clinical scores

Molecular epidemiology of bloodstream-associated Escherichia coli ST131 H30-Rx subclone infection in a region with high quinolone resistance

Genetic diversity of extraintestinal Escherichia coli strains producers of β-lactamases TEM, SHV and CTX-M associated with health-care

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