Toxicity of essential oils from leaves of Piperaceae species in rice stalk stink bug eggs, Tibraca limbativentris (Hemiptera: Pentatomidae)

Abstract

Tibraca limbativentris to is an important rice pest and occurs in all rice-growing regions of Latin America. The control this insect is accomplished with synthetic chemical insecticides, however, new approaches are needed to reduce risks to the environment, to the natural enemies and also to avoid the onset of insecticides resistance. This study was designed to assess the toxicity of essential oils (EOs) from leaves of Piper aduncum, P. gaudichaudianum, P. malacophyllum, P. marginatum and P. tuberculatum (Piperaceae) on rice stalk stink bug eggs, T. limbativentris. Essential oils were extracted with steam distillation and dilutions were made for bioassays at concentrations of 0.25; 0.5; 1.0; 2.0 and 4.0%. Essential oils from all species of Piperaceae displayed ovicidal activity. The LC 50 values indicated that both younger and older eggs were susceptible to these oils. Ovicidal activity is related to the potential toxicity of several compounds, especially dilapiolle, myristicin, cubebene, α-guaiene, longifolene, prezizane, spathulenol, sabinene and δ-2-carene. Thus, EOs tested showed promising results for use as biorational botanical insecticides. Index terms: Piper aduncum; Piper gaudichaudianum; Piper malacophyllum; Piper marginatum; Piper tuberculatum. RESUMOTibraca limbativentris é uma importante praga do arroz e ocorre em todas as regiões de cultivo de arroz da América Latina. O controle destes inseto é realizado com inseticidas químicos sintéticos, no entanto, novas abordagens são necessárias para reduzir os riscos para o ambiente, inimigos naturais, e também para evitar o aparecimento da resistência aos inseticidas. Este estudo foi concebido para avaliar a toxicidade dos óleos essenciais (OEs) das folhas de Piper aduncum, P. gaudichaudianum, P. malacophyllum, P. marginatum e P. tuberculatum (Piperaceae) sobre ovos do percevejo do colmo do arroz, T. limbativentris. Os OEs foram extraídos por arraste de vapor d´agua e a partir destes foram feitas diluições nas concentrações de 0,25; 0,5; 1,0; 2,0 e 4,0% para os bioensaios. Os OEs de todas as espécies de Piperaceae apresentaram atividade ovicida. Os valores de CL 50 indicaram que tanto os ovos mais jovens quantos os mais velhos foram suscetíveis aos diferentes óleos. Esta atividade ovicida pode estar relacionada com a toxicidade dos vários compostos encontrados em cada OE, como o dilapiol, miristicina, cubebeno, α-guaieno, longifoleno, prezizano, espatulenol, sabineno e δ-2-careno. Assim, os OEs testados mostraram resultados promissores para serem utilizados como inseticidas vegetais.

<b>Ovicidal effect of the essential oils from 18 Brazilian <i>Piper</i> species: controlling <i>Anticarsia gemmatalis</i> (Lepidoptera, Erebidae) at the initial stage of development

“…Piper aduncum and P. marginatum presented compounds already reported as insecticides in previous works, such as dillapiol, asaricine (sarisan), piperitone and myristicin (Bizzo et al, 2001;Morais et al, 2007;Qin, Huang, Li, Chen, & Peng, 2010;Souto et al, 2012;Santana et al, 2015;Ribeiro, Camara, & Ramos, 2016;Krinski & Foerster, 2016). For P. caldense, P. fuligineum, P. Mollicomum, and P. mosenii, there are no studies regarding the insecticidal activity, and our work is the first to test the ovicidal activity against a lepidopteran pest of importance in Brazilian agriculture.…”

“…It was shown that the chemical compounds present in these Piperaceae can be further evaluated among the isolation of active molecules to develop new botanical formulations, especially when we consider that the majority of these substances has been reported as potential insecticides in many other studies conducted worldwide (Santos et al, 2010;2013;Cáceres & Kato, 2014, Brito, Baldin, Silva, Ribeiro, & Vendramim, 2015Krinski & Foerster, 2016, Sanini et al 2017, Turchen, Hunhoff, Paulo,Souza, Pereira, 2016b and other plant families (Isman, 2000;Koul, Walia, & Dhaliwal, 2008;Tripathi, Upadhyay, Bhuiyan, & Bhattacharya, 2009;Coitinho, Oliveira, Gondim-Júnior, & Câmara, 2010;Ntalli & Menkissoglu-Spiroudi, 2011, Zoubiri & Baaliouamer, 2011Baskar, Muthu, Raj, Kingsley, & Ignacimuthu, 2012;Krishnappa & Elumalai, 2012;Cáceres & Kato, 2014Krinski, Massaroli, & Machado, 2014;Backiyaraj et al, 2015;Massaroli, Pereira, & Foerster;Costa, Santana, Oliveira, & Serrão, 2017).…”

Quantitative and qualitative damage caused by Oebalus poecilus (Hemiptera, Pentatomidae) to upland rice cultivated in new agricultural frontier of the Amazon rainforest (Brazil)

“…Besides this information, the producer must consider several other factors already reported in the studies of Barrigossi (2008), Tindall, Stout and Williams (2004) and Tindall et al (2005) aimed at integrated pest management, in addition to the use of alternative controls such as the use of phyto-insecticides and release of natural enemies (Favetti et al, 2013;Massaroli, 2014;Machado, 2014;Piton et al, 2014;Zachrisson;Costa;Bernal, 2014;Zachrisson et al, 2014;Turchen et al, 2016ab;Foerster, 2016;Martins;Sanini et al, 2017).…”

Reports of new wing color polymorphism and taxonomic information to cercopids (Auchenorrhyncha: Cercopidae) from upland rice crop, Pará State, Brazil

“…Given the economic importance of rice crop in Brazil, we recommend careful monitoring in rice fields and studies of control, biology, morphology, ecology and taxonomy of Cercopidae in different rice varieties, mainly to verify if some species can become a potential pest for rice cultivation in the future, as already observed for others pest for upland rice crops in this region (Krinski and Foerster, 2016;Martins and Krinski, 2016). on their property.…”