Optimization of jenipapo in vitro seed germination process

Souza, Rafaela Ribeiro de; Paiva, Patrícia Duarte de Oliveira; Silva, Raphael Reis da; Reis, Michele Valquíria dos; Nery, Fernanda Carlota; Paiva, Renato

doi:10.1590/1413-70542016406014816

Cited by 12 publications

(11 citation statements)

References 25 publications

(28 reference statements)

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“…rufa seeds exposed to GA 3 at different concentrations (0, 2, 8, 32 and 128 μM) in the culture medium showed no significant difference. Similar behavior was shown in Genipa americana L., a Brazilian fruit tree species in which no response to the application of exogenous GA 3 during its in vitro germination process was observed (Souza et al, 2016).…”

Section: Germinationsupporting

confidence: 72%

In vitro propagation of Campomanesia rufa: An endangered fruit species

et al. 2018

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The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3 was also tested on in vitro shoot elongation. For oxidation control, the medium was supplemented with PVP (polyvinylpyrrolidone) and for callus induction, 2.4-D (dichlorophenoxyacetic acid). The results showed 68% germination regardless of GA3 concentration. In the propagation stage, BAP at 4.5 µM induced a higher number of shoots (4.53) and LED lamps as the light source combined with the culture medium with 1 μM BAP induced a higher number of shoots (4.08). The highest average of C. rufa length (31.9 mm) was obtained using 8.8 µM GA3. The use of PVP (584.3 uM) controls up to 27.3% oxidation in young leaf explants. The use of 10 µM 2.4-D leads to a higher callus formation (58.7 %). Therefore, it can be concluded that the use of BAP is efficient in the induction of shoots, PVP controls oxidation leaf segments, and 2.4-D induces callus in C. rufa.

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Section: Germinationsupporting

confidence: 72%

In vitro propagation of Campomanesia rufa: An endangered fruit species

et al. 2018

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“…Higher concentrations of sucrose result in a considerable decrease in the medium osmotic potential [17]. According to de Souza et al [27], the germination of Genipa americana seeds necessitates the addition of sucrose for the maintenance of osmotic balance in the culture medium. To germinate, seeds must accumulate water potential during imbibition.…”

Section: In Vitro Seed Germinationmentioning

confidence: 99%

Germination and Early Growth of <em>Acacia nubica </em>Benth.<em> In vitro</em>

Ali¹,

Daffalla²,

Osman³

et al. 2022

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Acacia nubica is well-adapted to harsh conditions and offers valued socio-economic and environmental benefits. This research studied seed germination and seedling growth of A. nubica in vitro in response to the application of different concentrations of sodium hypochlorite, sucrose, and Murashige and Skoog (MS) medium. The synchrony, percentage, and rate of germination of A. nubica seed were studied. Seedling development was assessed by measuring the shoot, root, and number of nodes. Sodium hypochlorite at 10% produced the maximum germination percentage, while 5% induced a faster and more uniform emergence. Sucrose has various effects on seed emergence and germination depending on the concentrations; 30 g/L elicited the highest germination, while 10 g/L improved seed emergence rate with higher uniformity. The highest seed germination was achieved on ¼-MS medium, although 0-MS strength recorded faster and more synchronized germination. The development of A. nubica seedlings was influenced by MS medium strengths and sucrose concentrations. Full MS medium produced the highest shoot length and the highest vigor index with the addition of 20 g/L sucrose and the maximum root length with 50 g/L sucrose. Also, 30 g/L sucrose gave the maximum number of nodes and the maximum root/shoot ratio. However, ½-MS strength yields the highest seedling biomass with 30 g/L sucrose. This is the first successful report on the seed germination and seedling growth of A. nubica in vitro.

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“…Metabolite biosynthesis, in addition to presenting specificity in relation to tissue and organ, is also regulated by environmental conditions, and its levels may be increased, thus increasing the yields of various compounds or classes of metabolites of medicinal interest. Thus, in vitro cultivation is a very useful and successfully exploited tool, either to provide a better understanding of the biological mechanisms involved between the physical environment and plants or to increase the metabolic levels or specificity (Ouzounis et al, 2015;Batista et al, 2016;Souza et al, 2016).…”

Section: Total Phenolic Content and Hplc-dadmentioning

confidence: 99%

“…Although studies have shown the influence of light quality on plant physiology and behavior, the light spectrum-dependent responses for each species should also be analyzed, as these responses vary greatly and may have different patterns (Batista et al, 2016;Souza et al, 2016;Li et al, 2017;Batista et al, 2018). This study analyzed how light spectrum quality affects growth, chlorophyll, and total phenolic content in M. oleifera plants grown in vitro.…”

Section: Introductionmentioning

confidence: 99%

Light quality on growth and phenolic compounds accumulation in Moringa oleifera L. grown in vitro

et al. 2020

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All plant parts of the Moringa oleifera can contain relevant concentrations of phytochemicals with health benefits. Plants grown in vitro allow pathogen-free plant material production and rapid propagation, and this technique is widely used to obtain secondary metabolites. This study analyzed how light spectrum quality affects growth, chlorophyll, and total phenolic content in M. oleifera plants grown in vitro. M. oleifera seeds were inoculated in MS medium supplemented with 3% sucrose and 0.7% agar and were stored under a controlled temperature, humidity and photoperiod. The light conditions tested were white fluorescent lamps (WFL) and light-emitting diodes (LED: 70% red + 30% blue), both standardized with a photon flux density of 58 μmol m-2s-1. Radiation emitted by WFL and LED did not influence stem germination, height, or diameter. However, WFL provided higher total chlorophyll levels. All plant parts germinated in vitro were analyzed via high-performance liquid chromatography with a diode-array detector (HPLC-DAD), and preliminary analyses revealed preferential synthesis of gallic acid derivatives in the leaves under LED radiation. For the total phenolic content, leaves under the LED and WFL irradiations showed 3.524 ± 0.054 and 3.805 ± 0.304 micrograms, respectively, of gallic acid equivalents per milligram of dry matter (μg GAE mg-1 DM). Light quality did not interfere with phenolic compound accumulation; however, it stimulated the preferential synthesis of gallic acid derivatives in leaves grown under LED radiation, and the evidence indicated that all evaluated organs synthesized nonpolar substances.

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Optimization of jenipapo in vitro seed germination process

Cited by 12 publications

References 25 publications

In vitro propagation of Campomanesia rufa: An endangered fruit species

In vitro propagation of Campomanesia rufa: An endangered fruit species

Germination and Early Growth of <em>Acacia nubica </em>Benth.<em> In vitro</em>

Light quality on growth and phenolic compounds accumulation in Moringa oleifera L. grown in vitro

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