Atividade enzimática durante a germinação de sementes de Melanoxylon brauna schott sob diferentes temperaturas

Flores, Andressa Vasconcelos; Borges, Eduardo Euclydes de Lima e; Guimarães, Valéria Monteze; Gonçalves, José Francisco de Carvalho; Ataíde, Glauciana da Batista; Barros, Daniela de Pádua

doi:10.1590/01047760201420031399

Cited by 20 publications

(20 citation statements)

References 21 publications

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“…The same behavior is expected for CAT enzyme activity since it acts on the products processing derived from SOD activity. This increased activity of both enzymes during the germination process has been reported in a study by Flores et al (2014) with seeds of Melanoxylon brauna, where they observed increased activity of the two enzymes with longer exposure of the seeds to imbibition, and by Carneiro et al (2011), with sunflower seeds in NaCl solutions.…”

Section: Introductionsupporting

confidence: 79%

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

et al. 2017

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ABSTRACT. With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame seeds under different water availability. To simulate the water restriction we used PEG6000, a high molecular weight molecule that does not penetrate the seed structure but allows different osmotic potentials. The treatments were -0.1, -0.2, and -0.3 MPa, and the control. Germination, first-count germination, germination velocity index, and length and dry mass of the hypocotyl and radicle were performed. The seeds were weighed before and after treatments every 3 h. After each weighing, 100 seeds were taken for analysis of the enzymes alcohol dehydrogenase (ADH), malate dehydrogenase, esterase, catalase (CAT), superoxide dismutase (SOD), isocitrate lyase (ICL), and glutamate dehydrogenase (GTDH). The statistical design was completely randomized with five replications. PEG6000 prolonged ADH activity during the beginning of germination, maintaining the anaerobic metabolism for longer. Subsequently, their activity was reduced, as well as ICL, favoring the deterioration of the seeds that take the time to germinate. Behavior was evidenced by the appearance of SOD, CAT, and GTDH isoforms after 24 h of imbibition when water restriction was imposed. Therefore, the PEG600 is efficient in simulating water deficit conditions in future scenarios of climate change, offering impotent information regarding the germination behavior of the plants under these conditions.

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Section: Introductionsupporting

confidence: 79%

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

et al. 2017

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“…Superoxide dismutase (SOD) and catalase (CAT) enzymes -The crude enzyme extract used were obtained from the method described by Flores et al (2014a). Three replicates of 0.1 g for each sample withdrawal time were used for both enzymes.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, it is in the Brazilian List of Endangered Flora Species (MMA, 2008). Despite the great economic and environmental value and studies involving aspects related to the seed species physiology (Flores et al, 2014a;Flores et al, 2014b;Borges et al, 2015), more scientific research about the spread of M. brauna is needed, particularly with an emphasis on dehydration and germination steps.…”

Section: •-mentioning

confidence: 99%

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

2015

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-Germination is a complex process that involves molecules properties that make up the cell walls, hydrolytic enzymes that break the bonds between the polymers and action of reactive oxygen substance. Melanoxylon brauna is a forest species of high economic value. In order to evaluate the physiological and biochemical changes that occur in the embryonic axis during germination, fresh matter, length, activities of the enzymes pectin methylesterase, polygalacturonase, superoxide dismutase, catalase, peroxidase and hydrogen peroxide levels were quantified in the embryonic axis. Furthermore, in the micropyle area the composition of carbohydrates and micropyle physical resistance were evaluated with and without drying. During soaking, if there are increases in fresh matter and length of the embryonic axis, there is the same trend of polygalacturonase and pectin methylesterase enzymes. The hydrogen peroxide content was reduced during the soaking, as well as the puncture force of the micropylar area. It is concluded that the seed coat and the cotyledons are responsible for 90% of the water soaked by the seeds. The events in the micropyle and embryonic axis occur independently in the first 16 hours. The weakening of the micropyle features an elastic step and a plastic one. Enzymes pectin methylesterase and polygalacturonase act in cellular expansion of the embryonic axis.Index terms: puncture force, hydrogen peroxide, cell wall, germination.Eventos micropilar e embrionário na hidratação de sementes de Melanoxylon brauna Schott RESUMO -A germinação é um processo complexo que envolve propriedades das moléculas que compõem a parede celular, enzimas hidrolíticas que quebram ligações entre polímeros da pectina e da hemicelulose e ação das substâncias reativas de oxigênio. Melanoxylon brauna é uma espécie florestal de alto valor econômico. Com o objetivo de avaliar alterações fisiológicas e bioquímicas no eixo embrionário durante a germinação, foram quantificados a massa fresca, o comprimento, as atividades das enzimas pectina metilesterase, poligalacturonase, superóxido dismutase, catalase, peroxidase e os teores de peróxido de hidrogênio. Foram avaliados na região micropilar a composição dos carboidratos e a resistência da micrópila seca e sem secagem. Durante a embebição, ocorrem aumentos na massa fresca e comprimento dos eixos embrionários, mesma tendência das enzimas poligalacturonase e pectina metilesterase, que participam da expansão celular. O conteúdo de peróxido de hidrogênio reduziu durante a embebição, assim como a força de ruptura da região micropilar. Conclui-se que o tegumento e os cotilédones são responsáveis por 90% da água embebida pelas sementes. Os eventos na micrópila e eixo ocorrem independentes nas primeiras 16 horas. O enfraquecimento da micrópila apresenta uma etapa elástica e outra plástica.As enzimas pectina metilesterase e a poligalacturonase atuam na expansão celular do eixo embrionário.Termos para indexação: força de ruptura, peróxido hidrogênio, parede celular, germinação.

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“…In addition, it reduces the activity of enzymes related to the germination process -lipase, amylase, and protease -and increases the activity of enzymes related to stress -superoxide dismutase and catalase (Marcos-Filho, 2005;Flores et al, 2014). While at a higher temperature up to a certain point, it contributes to accelerating the speed of water uptake and also to reactions that determine all the biochemical and enzymatic processes involved in germination (Marcos-Filho, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…The temperature affects moisture uptake and regulates the biochemical and enzymatic reactions that regulate metabolism, reactions that are involved in the germination process (Bewley et al, 2014;Flores et al, 2014). Germination is variable, depending on the species and even within cultivars of the same species (Andrade and Jasper, 2013;Lone et al, 2014); each species requires an optimal range of temperature for this process to occur in the most efficient manner.…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature on passion fruit emergence and seedling vigor

et al. 2017

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-Seedlings for commercial growing of passion fruit are preferentially acquired through seeds. Success in germination and seedling emergence, in turn, is influenced by the genetic constitution of the progenies and the thermal conditions imposed on the growth environment. The aim of this study was to evaluate the emergence and vigor of seedlings of passion fruit cultivars exposed to different temperatures. The experiment was conducted in a germination chamber at the Unidade de Ensino, Pesquisa e Extensão / Pomar Campus of the Department of Plant Science of the Universidade Federal de Viçosa (UFV). The study was carried out in 5 × 3 factorial arrangement, with five temperature ranges (5-15, 10-20, 15-25, 20-30 and 25-35 °C) and three varieties of passion fruit (BRS Sol do Cerrado, BRS Gigante Amarelo and FB-200 Yellow Master) in a completely randomized design with six replications of 25 seeds per plot. The variables analyzed were the percentage, the speed index, and the average time of emergence, shoot length, total length, and seedling dry matter. The cultivars BRS Sol do Cerrado and BRS Gigante Amarelo exhibited seedling emergence of over 95% in the temperature ranges of 20-30 °C and 25-35 °C. Alternating temperatures from 20-30 °C stimulates seedling emergence, seedling growth, and greater accumulation of seedling dry matter.Index terms: Passiflora edulis Sims, cultivars, seeds, thermal conditions. Emergência e vigor de plântulas de maracujazeiro azedo em função da temperatura RESUMO -A propagação de plantas de maracujazeiro azedo, em plantios comerciais, é realizada preferencialmente por meio de sementes. O sucesso da germinação e emergência de plântulas, por sua vez, é influenciado pela constituição genéticas das progênies e pelas condições térmicas impostas no ambiente. O objetivo neste trabalho foi avaliar a emergência e o vigor de plântulas de cultivares de maracujazeiro azedo sob exposição de diferentes temperaturas. O experimento foi conduzido em câmara de germinação na Unidade de Ensino, Pesquisa e Extensão/Pomar Campus pertencente ao Departamento de Fitotecnia da UFV. O trabalho foi desenvolvido em esquema fatorial 5 × 3, com cinco faixas de temperatura (5-15; 10-20; 15-25; 20-30 e 25-35 °C) e três cultivares de maracujazeiro azedo (BRS Sol do Cerrado, BRS Gigante Amarelo e FB-200 Yellow Master) no delineamento inteiramente casualizado e seis repetições de 25 sementes por parcela. As variáveis analisadas foram as percentagem, índice de velocidade e tempo médio de emergência, o comprimento da parte aérea e total e massa seca de plântula. As cultivares BRS Sol do Cerrado e BRS Gigante Amarelo apresentaram emergência de plântulas acima de 95% nas faixas de temperaturas de 20-30 °C e 25-35 °C. Temperaturas alternadas 20-30 °C estimulam a emergência, o crescimento e maior acúmulo de massa seca das plântulas.

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Atividade enzimática durante a germinação de sementes de Melanoxylon brauna schott sob diferentes temperaturas

Cited by 20 publications

References 21 publications

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

Effect of temperature on passion fruit emergence and seedling vigor

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