Ethanol Precipitation as a Downstream Processing Step for Concentration of Xylanases Produced by Submerged and Solid-State Fermentation

Abstract

Xylanases have applications in different industries, being produced by microbial cultivations using submerged (SmF) or solid-state fermentation (SSF). Precipitation stands out as a potential method for the concentration of xylanases, especially with the use as ethanol as the precipitant due to its compatibility with the biorefinery concept. This paper presents a comparative laboratory scale study of ethanol precipitation of xylanases produced by Aspergillus niger cultivated under SSF and SmF. Precipitation conditions were selected according to a central composite design. Statistical analysis showed a significant effect of pH on the recoveries of total protein and xylanase activity. The kinetic profiles showed that a relatively short period of time (up to 15 min) was sufficient to recover most of the xylanase activity precipitated under the selected conditions. Xylanase recoveries of 65 and 79% were achieved for the SSF and SmF enzymatic complexes, respectively.

Bioremediation potential of industrial laundry effluent by agaricomycetes from brazilian tropical dry forest

“…The enzymes present in the extracts obtained by the liquid fermentation of F1, F5 and FM fungi were concentrated by ethanolic precipitation of proteins according to the methodology (modified) of Costa et al (2018). A volume of 70 mL of absolute ethanol previously refrigerated at 4 o C was used, which was slowly added to 30 mL of the enzymatic extract under constant stirring.…”