Silver nanoparticles (Ag-NPs) are known for their efficient bactericidal activity and are widely used in industry. This study aims to produce printable antibacterial devices by drop-on-demand (DoD) inkjet technology, using Ag-NPs as the active part in complex printable fluids. The synthesis of this active part is described using two methods to obtain monodisperse NPs: chemical and microwave irradiation. The synthesized NPs were characterized by UV-VIS, STEM, TEM, DLS and XRD. Two printable fluids were produced based: one with Ag-NPs and a second one, a polymeric nanocomposite, using silver nanoparticles and polyvinyl butyral (Ag-NPs/PVB). Cellulose acetate was used as a flexible substrate. The ecotoxicity analysis of fluids and substrate was performed with Artemia franciscana nauplii. Optimized electric pulse waveforms for drop formation of the functional fluids were obtained for the piezoelectric-based DoD printing. Activity of printed antibacterial devices was evaluated using the Kirby-Bauer method with Staphylococcus aureus and Escherichia coli. The results show that the printed device with Ag-NP fluids evidenced a bacterial inhibition. An important advantage in using the DoD process is the possibility of printing, layer by layer or side by side, more than one active principle, allowing an interleaved or simultaneous release of silver NP and other molecules of interest as for example with a second functional fluid to ensure effectiveness of Ag activity.
Mycotic bioremediation of effluents from industrial jeans laundries is a necessary biotechnological treatment to prevent contamination of water bodies. In phase I, the discoloration of Indigo Carmine Dye (ICD) and Textile Effluent (TE) by seven species of Agaricomycetes from the brazilian tropical dry forest (Caatinga) was evaluated. First, nutritional stress was caused by Nitrogen Limitation (NL) at three experimental times, T1 (1 day). T2 (4 days) and T3 (7 days). In phase II, microorganisms were cultivated in the initial growth times Gi1 (10 days) and Gi2 (25 days), Without Addition of Nutrients (WAN) and stress was induced by NL (T1). Subsequently, ICD and TE discoloration tests continued for 28 days. In the ecotoxic analysis, the biotreated samples in phase II were tested on nauplii of Artemia HIGH 5 without the addition of food. In phase I, the percentages of ICD and TE discoloration were greater than 55% using fungi F1, F2, F5 and F6 for 10 days without sterility. In phase II, the best percentages of discoloration were found for TE in Gi1 and for ICD in Gi2, with F1 and F5 (identified by molecular biology). The results showed that Gi1 (WAN) increased the biodegradation of TE and Gi2 (WAN) favored the biodegradation of ICD, in T1 (NL) without sterility. The best enzymatic activity of laccase and lignin peroxidase was presented in F5. The enzyme extracts had a Michaelis-Menten kinetic behavior. All samples of TE bioremediated in phase II no showed toxicity on Artemia sp. in 48 hours of experimentation.
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