Regulators, culture media and types of lights in vitro lavender culture

Abstract

Lavender is an aromatic ornamental plant that is used widely in the perfume, pharmaceutical, cosmetical and food industries. That is why it is important to study ways to promote a production of lavender raw material. For this, four experiments were carried out in order to study the germination and initial in vitro development of lavender. In the first part the efficiency of the use of hydrogen peroxide in the pre-treatment of seeds inoculated in culture media with different concentrations of gibberellic acid was evaluated. Besides that, the influence of the use of Growlux lamps and white fluorescent lamps on plantlets cultivated in MS and LS media at salts concentrations of 50 and 100% was evaluated. Finally, the effect of the gibberellic acid, putrescine, spermine and spermidine in different concentrations was evaluated. Better results were estimated when hydrogen peroxide pretreatment was applied to the seeds with subsequent inoculation in a medium containing 2,5 mg L-1 of gibberellic acid. The use of Growlux lamps did not influence the characteristics observed when compared to the use of white fluorescent lamps, however it was concluded that the species develops more in a LS medium. Concerning the use of polyamines was found that germination, aerial part and number of leaves of the seedlings were generally favored at the concentration of 0,5 mg L-1 except when spermine was applied, which resulted in a lower number of leaves under this condition.

Micropropagation and Acclimatization of Common Oregano (<i>Origanum vulgare</i> L. Subsp. <i>vulgare</i>) by Shoot Tip Culture

“…for hypocotyls, the combination of 0.01 mM spermidine and 27 µM NAA increases root induction. Oliveira et al (2019) [39] showed that among the polyamines tested on the in vitro growth of Lavandula angustifolia L. vitroplants, 5,67 µM of putrescine and spermidine promote the in vitro culture of explants. Frabetti et al GA 3 combined with 3.11 µM Kin + 6.27 µM IBA improves the growth of Origanum vulgare vitroplants, especially in the propagation phase for the vegetative part.…”

Melhoramento da rosa-do-deserto

“…O uso do regulador de crescimento GA3 (ácido giberélico) na germinação in vitro é uma alternativa para promover uniformidade, aumento na porcentagem de germinação e vigor das plântulas em condições in vitro. Neste sentido, esse regulador tem sido testado no cultivo in vitro de espécies ornamentais, como a lavanda (ASMAR et al, 2019), sempre-viva (PRUDENTE et al, 2015), orquídea (SOARES et al, 2009), entre outras. Para a Rosa-do-Deserto, a adição do GA3 ao meio de cultura, foi indiferente na germinação das sementes (VARELLA et al, 2015).…”

Effect of light spectra on stem cutting rooting and lavender growth

“…Light spectral composition is as important for plant growth as intensity because each spectrum range influences a specific plant receptor (Spalholz, Perkins-Veazie, & Hernández, 2020). It is possible to observe different plant responses depending on the light spectrum range from seed germination (Oliveira, Asmar, Silva, Morais, & Luz, 2019), growth and elongation (Li et al, 2017) to plant mass (Nájera & Urrestarazu, 2019). When natural lighting is limited or absent, artificial lamps can assist plant growth (Bantis & Radoglou, 2019;Wei, Liu, Hu, & Jeong, 2020).…”