Inoculation methods and aggressiveness of five Fusarium species against peach palm

Jarek, Tiago Miguel; Santos, Á. F. dos; Tessmann, Dauri José; Vieira, Elisa Serra Negra

doi:10.1590/0103-8478cr20170462

Cited by 7 publications

(6 citation statements)

References 3 publications

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“…Fusarium oxysporum and F. proliferatum are frequently isolated from the same diseased plants, e.g. in asparagus (Borrego-Benjumea et al 2014), soybeans (Chang et al 2015), and peach palm (Jarek et al 2018), similar to what was observed in this study. Dual inoculations with both species were not conducted given the severe symptoms produced by each individual species.…”

Section: Discussionsupporting

confidence: 88%

“…Fusarium proliferatum is a member of the Gibberella fujikuroi species complex, which is comprised at least of 15 reproductively isolated biological species (mating populations), and infects a broad range of plant species and produces a range of mycotoxins (Leslie et al 2004;Niehaus et al 2016). Some of the more economically important host species affected by F. proliferatum are asparagus (Elmer 1990(Elmer , 1995Borrego-Benjumea et al 2014), alfalfa (Cong et al 2016), banana (Jimenez et al 1993), citrus fruits (Hyun et al 2000), corn (Logrieco et al 1995;Munkvold 2003), garlic (Dugan et al 2003;Palmero et al 2012;Galvez et al 2017), mango (Zhan et al 2010), onion (Stankovic et al 2007;Carrieri et al 2013), orchids (Benyon et al 1996), peach palm (Jarek et al 2018), pistachio (Crespo et al 2019), rice (Desjardins et al 2000;Kim et al 2012;Quazi et al 2013;Choi et al 2017Choi et al , 2018, safflower (Kim et al 2016), sesame (Torabi et al 2014), soybean (Chang et al 2015), sorghum (Leslie 2003), strawberry (Borrero et al 2019), and wheat (Jurado et al 2006;Conner et al 2009). Symptoms on these hosts included damping-off, wilting, yellowing of leaves, soft rot, root and crown rot, vascular discoloration, as well as blackening of the crown and roots of diseased plants as seen on palm and strawberry plants (Jarek et al 2018;Borrero et al 2019).…”

Section: Discussionmentioning

confidence: 99%

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First report of Fusarium proliferatum causing crown and stem rot, and pith necrosis, in cannabis (Cannabis sativa L., marijuana) plants

Punja

2020

Canadian Journal of Plant Pathology

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Cannabis (Cannabis sativa L., marijuana) plants grown under greenhouse or controlled environments with symptoms of leaf yellowing, leaf necrosis and defoliation were observed during 2018-2019. Additional symptoms included crown rot and internal browning or blackening of the pith tissues. Stock (mother) plants as well as plants in the vegetative and flowering stages of 15 cannabis strains (genotypes) were affected. In addition, damping-off symptoms were observed on rooted cuttings in propagation rooms. Isolations from diseased tissues yielded predominantly Fusarium proliferatum, with some F. oxysporum also recovered. Phylogenetic analysis of sequences from the translation elongation factor 1α (TEF-1 α) region of 29 isolates of F. proliferatum from eight licenced production facilities in three provinces in Canada (British Columbia, Ontario and New Brunswick), and one cannabis production site in northern California, grouped isolates from cannabis with a large clade of isolates from a wide range of other hosts in different geographic regions. Pathogenicity studies confirmed the ability of F. proliferatum to cause symptoms of wilting, leaf and pith necrosis, and plant death on cuttings, rooted plants and stock plants. Inoculated tomato and cucumber plants developed similar symptoms. Stem colonization was more extensive by F. proliferatum compared to F. oxysporum on cannabis cuttings. Both grew optimally at 25°C on agar media although F. oxysporum grew faster than F. proliferatum at all temperatures tested. The occurrence of F. proliferatum on cannabis plants has not been previously reported, adding to recent reports of F. oxysporum and F. solani that cause similar symptoms on cannabis plants.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

First report of Fusarium proliferatum causing crown and stem rot, and pith necrosis, in cannabis (Cannabis sativa L., marijuana) plants

Punja

2020

Canadian Journal of Plant Pathology

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“…The use of a mycelial+spore suspension as inoculum provided more consistent results compared to a spore suspension, and an inoculation time of 3 min gave consistent but not excessive levels of disease compared to 5 min on both strains evaluated (unpublished observations). The use of an inoculum concentration in the range of 1 × 10 6 -1 × 10 7 CFU mL −1 and exposure times of 3-5 min is comparable to inoculation methods used by other researchers to study host-pathogen interactions in a range of other crop species infected by F. oxysporum (Purwatti et al 2008;Ortiz and Hoyos-Carvajal 2016;Paynter and Herrington 2016;Jarek et al 2018). The symptoms resulting from the inoculation of cuttings was identical to those seen in the commercial propagation room.…”

Section: Discussionmentioning

confidence: 76%

Epidemiology of Fusarium oxysporum causing root and crown rot of cannabis (Cannabis sativa L., marijuana) plants in commercial greenhouse production

Punja

2020

Canadian Journal of Plant Pathology

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Fusarium oxysporum causes root browning and crown infection on marijuana (Cannabis sativa L.) plants, resulting in stunted growth, yellowing of leaves, and plant death. Pathogen presence and diversity were assessed in samples of diseased crowns, stems, pith tissues and roots from five commercial production facilities in British Columbia and Ontario. PCR of the elongation factor (EF-1 α) region and sequence analysis confirmed the identity and phylogenetic relationships among 33 representative isolates from over 200 isolates collected. Stock (mother) plants of eight cannabis strains (genotypes) with symptoms of yellowing, crown rot and internal stem decay yielded F. oxysporum at a frequency of 70-100%. Cuttings obtained from asymptomatic plants showed pathogen recovery rates of 1-2% on potato dextrose agar at distances of 90-150 cm from the crowns. Symptoms of damping-off were observed on cuttings in a rooting and propagation facility. Pathogenicity tests confirmed the development of characteristic symptoms on cuttings and rooted plants inoculated with F. oxysporum. Phylogenetic analysis indicated that isolates of F. oxysporum from BC belonged to a separate clade from ON isolates and that clonal spread was occurring within licenced facilities. Air sampling conducted in a greenhouse facility revealed F. oxysporum was present in areas used for rooting, vegetative propagation, and where stock plants were grown. The pathogen was also recovered from inflorescences. These findings indicate that F. oxysporum is present in several commercial production facilities in Canada and reduces root development and establishment of rooted cuttings, and causes yellowing and stunting on flowering plants of many cannabis strains.

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“…The use of Fusarium -colonized ground corn for root inoculation was effective and reduced the level of damage to plants. In this sense, the authors report that root inoculation with Fusarium -colonized ground corn was the most suitable method for evaluating the fusariosis 42 . Aditionally, Rully et al (2008) evaluated the effectiveness of inoculation methods for F. oxysporum f.sp.…”

Section: Discussionmentioning

confidence: 99%

Fusarium spp. associated with Chenopodium quinoa crops in Colombia

Fonseca-Guerra

Pompeyo

Rozo

et al. 2022

Sci Rep

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Quinoa is a plant commonly-resistance to adverse biotic and abiotic factors. However, this crop can be affected by phytopathogenic fungi. There is a lack of knowledge about the fungi associated with quinoa plants in Colombia. Through morphological and molecular identification in this study were identified four Fusarium species associated with quinoa crops: Fusarium oxysporum, Fusarium graminearum, Fusarium equiseti, and Fusarium culmorum. For this, we collected samples of panicles, leaf tissue, root tissue, and soil for isolation of different isolates of Fusarium. We performed a pathogenicity test of the fungi strains, under greenhouse conditions to evaluate the pathogenicity in seedlings of the Piartal cultivar with two inoculation methods. First inoculating the stem through a nodal wound or second inoculating the abaxial face with a brush. The results indicate the presence of four species with both molecular markers, phylogenetically distributed in these groups. The four species turned out to be pathogenic but with different degrees of virulence with significant differences between F. graminearum and F. oxysporum depending on the inoculation method. This is the first report on the presence of Fusarium species isolated from Quinoa in Colombia.

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Inoculation methods and aggressiveness of five Fusarium species against peach palm

Cited by 7 publications

References 3 publications

First report of Fusarium proliferatum causing crown and stem rot, and pith necrosis, in cannabis (Cannabis sativa L., marijuana) plants

First report of Fusarium proliferatum causing crown and stem rot, and pith necrosis, in cannabis (Cannabis sativa L., marijuana) plants

Epidemiology of Fusarium oxysporum causing root and crown rot of cannabis (Cannabis sativa L., marijuana) plants in commercial greenhouse production

Fusarium spp. associated with Chenopodium quinoa crops in Colombia

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