Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats

Abstract

To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time.

RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

“…MSCs are pluripotent cells that exist in a variety of tissues (bone marrow, cord blood and cord tissue, placental tissue, etc.) [17]. MSCs have the potential for multi-directional differentiation, and a unique cytokine secretion function.…”

Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Nanoscaffold on Wound Healing in Irradiated Rats

“…Sacrifice was performed with overdose anesthetic injections. 16 The wounded site was excised in a circular pattern, with normal tissue included in skin specimens, and then fixed in 10% buffered formalin and processed using a paraffin tissue processing machine. Tissue specimens were cut into 5 µ thick sections, mounted on glass slides, stained with hematoxylin and eosin (H&E) stain, and examined under a light microscope to evaluate histological changes during the healing process.…”

In vivo study of wound healing processes in Sprague-Dawley model using human mesenchymal stem cells and platelet-rich plasma

“…Previous studies reported the benefits of MSCs in the cases of calvarial defects, Crohn's disease, critical limb ischemia, and multiple sclerosis, as well as in enhancement of wound healing 2 . Numerous studies have reported on the efficacy of autologous and allogeneic MSCs in applications to improve the wound healing process in various wound models 2- 15 . The effectiveness of MSCs to promote wound healing has also been proven in xenogenic applications [16][17][18][19][20] .…”