RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Abstract

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII +/+ (WT) and PrxII −/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII −/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII −/− vs. PrxII +/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

Loss of function of ribosomal protein L13a blocks blastocyst formation and reveals a potential nuclear role in gene expression

“…Therefore, we could not identify any embryos in our regression analysis with absolute zero reads of Rpl13a transcripts. This type of observation is not unprecedented and was reported by others 37 . Nonetheless, our Rpl13a ‐based regression analysis revealed widespread alterations in gene expression that could be mined as a resource via biological pathway analysis.…”

“…This type of observation is not unprecedented and was reported by others. 37 Nonetheless, our Rpl13a-based regression analysis revealed widespread alterations in gene expression that could be mined as a resource via biological pathway F I G U R E 1 Embryos with the Rpl13a null allele arrest at the morula stage. (A) PCR genotyping of mouse preimplantation embryos to detect the Rpl13a KO allele.…”

Induction of Targeted Differentiation of Dermal Mesenchymal Stem Cells Into Neural Lineage According to Peroxiredoxin II Expression

“…Preliminary laboratory studies have shown that Prx II-deficient DMSCs have a more pronounced effect on trauma treatment by increasing exosome secretion, which suggests the potential advantages of using Prx II -/-DMSCs to treat diseases. RNA sequencing results for Prx II +/+ DMSCs and Prx II -/-DMSCs have shown that Prx II -/-DMSCs are less immunogenic, which reduces the risks of stem cell therapy (19). In this study, we compared RNA sequencing results from Prx II +/+ DMSCs and Prx II -/-DMSCs.…”

Network analysis for the identification of hub genes and related molecules as potential biomarkers associated with the differentiation of bone marrow-derived stem cells into hepatocytes

“…The soft tissue on the surface of the mouse femur was carefully peeled off and the femur tissue was rinsed several times with a 1 mL syringe containing α-MEM culture solution, until it was transparent. Four rinsed femur cells were cultured in a medium containing BMSCs growth medium placed in a 95% air/5% CO2, 37° C incubator, and refreshed every three days [42][43][44].…”