Antibioticoterapia em microplantas de abacaxizeiro (Ananas comosus)

Leone, Gabriela Ferraz; Almeida, Cristina Vieira de; Abreu-Tarazi, Monita Fiori de; Batagin-Piotto, Katherine Derlene; Artioli-Coelho, Fabiane Aparecida; Almeida, Marcílio de

doi:10.1590/0103-8478cr20130958

Cited by 4 publications

(2 citation statements)

References 10 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The response of orchid seeds in in vitro germination is influenced by many factors, such as seed age, culture medium composition (carbohydrate source, antioxidants, plant growth regulators), culture condition and genotype (PARK et al, 2018;SEON et al, 2018). However, contamination by microorganisms (fungal and/or bacterial) is an issue associated with micropropagation, because these microorganisms establish themselves in the culture medium and/or in the vegetal material and cause toxicity due to the high production of toxic metabolites, as well as to pH reduction in the culture medium, which, consequently, leads to lower absorption of macro and micronutrients by plants and, unfortunately, to death (LEONE et al, 2016).…”

Section: Activated Charcoal In the In Vitro Germinationmentioning

confidence: 99%

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Souza

Fernandes

Molinari

et al. 2021

NAT

View full text Add to dashboard Cite

Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

show abstract

Section: Activated Charcoal In the In Vitro Germinationmentioning

confidence: 99%

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Souza

Fernandes

Molinari

et al. 2021

NAT

View full text Add to dashboard Cite

show abstract

“…1) e prejudicam o desenvolvimento das espécies, tornando-se vitropatogênicas (produzindo sintomas visíveis nas plantas e/ou com crescimento visível e de grande extensão no meio de cultura) (Thomas & Prakash 2004;Thomas 2006;Thomas et al 2006Thomas et al , 2007Moreno-Vázquez et al 2014), o mais recomendável é o descarte do material, desde que não seja imprescindível mantê-lo. Nesse último exemplo, quando a manutenção é necessária, podem ser feitos tratamentos curativos adicionando ao meio de cultivo antibióticos (antibioticoterapia), como ampicilina sódica, agrimicina, cloranfenicol, ácido nalidíxico, gentamicina e cefazolina, por períodos que variam de dias a meses (Pereira et al 2003(Pereira et al , 2014Thomas 2011;Leone et al 2016).…”

Section: Contaminação Versus Manifestaçãounclassified

Contaminação versus manifestação endofítica: implicações no cultivo in vitro de plantas

Esposito-Polesi

2020

Rodriguésia

View full text Add to dashboard Cite

Resumo A cultura de tecidos vegetais é imprescindível à propagação e multiplicação uniforme de plantas, à conservação de germoplasma, a programas de melhoramento e à transformação genética. Essa técnica tem exigido, cada vez mais, estudos que colaborem com o entendimento dos mecanismos envolvidos no crescimento dos microrganismos nos meios de cultivo, bem como as relações que eles estabelecem com a planta hospedeira. Dessa maneira, a presente revisão pretende esclarecer esses questionamentos e promover a distinção entre contaminação e manifestação endofítica que ocorrem no cultivo in vitro por diferentes causas. Tal distinção permite diminuir o pânico que se instala quando do seu aparecimento, além de auxiliar na adoção de medidas de prevenção e/ou controle desses eventos sem que haja descartes desnecessários de material de alto valor comercial e genético.

show abstract

Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F.Muell.: a micropropagation approach

Leone

Andrade

Almeida

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Antibioticoterapia em microplantas de abacaxizeiro (Ananas comosus)

Abstract: RESUMO Com o intuito de avaliar os efeitos da antibioticoterapia no crescimento de plantas micropropagadas de

Cited by 4 publications

References 10 publications

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Contaminação versus manifestação endofítica: implicações no cultivo in vitro de plantas

Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F.Muell.: a micropropagation approach

Contact Info

Product

Resources

About