Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F.Muell.: a micropropagation approach

Leone, Gabriela Ferraz; Andrade, Pedro Avelino Maia de; Almeida, Carolina Vieira De; Almeida, Cristina Vieira de; Andreote, Fernando Dini; Almeida, Marcílio de

doi:10.1007/s11627-019-09986-2

Cited by 7 publications

(9 citation statements)

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“…Endophytes are a class of endosymbiotic microorganisms that internally inhabit plant tissues [ 1 ]. Being protected inside explant or seed tissues, endophytic bacteria evade surface sterilization procedures used for cell culture initiation, and they are common in plant tissues grown in vitro [ 2 , 3 , 4 ]. Some bacterial endophytes maintain habitually concealed lifestyles or, due to limited bacterial growth-supporting media conditions, remain latent over extended periods of in vitro tissue cultivation.…”

Section: Introductionmentioning

confidence: 99%

“…To eliminate contamination of endophytic origin, pretreatment with high dose [ 14 ] or medium supplementation with microbial growth-inhibiting [ 15 ] antibiotics have been used and are often reported to improve the propagation or regenerative properties of the tissues [ 4 , 16 , 17 , 18 ]. However, antibiotics could be cytotoxic to plant tissues at concentrations required for efficient control of the bacterium, usually in the range of 100 to 500 mg L −1 [ 19 ] and could lead to tissue browning [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, at higher antibiotic concentrations, proline accumulation, oxidative injury, and reduced antioxidative activity have been reported, which indicates an elevated stress level [ 30 ]. Previously, the low cytotoxic effect of the antibiotic timentin on in vitro plant tissues has been described [ 4 , 26 ], and it is commonly used for transformation procedures in the range of concentrations from 150 mg L −1 [ 26 ] to 500 mg L −1 [ 31 ], sometimes in combination with lower concentrations of other antibiotics [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Enduring Effect of Antibiotic Timentin Treatment on Tobacco In Vitro Shoot Growth and Microbiome Diversity

Tamošiūnė

Andriūnaitė

Vinskienė

et al. 2022

Plants

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Plant in vitro cultures initiated from surface-sterilized explants often harbor complex microbial communities. Antibiotics are commonly used to decontaminate plant tissue culture or during genetic transformation; however, the effect of antibiotic treatment on the diversity of indigenous microbial populations and the consequences on the performance of tissue culture is not completely understood. Therefore, the aim of this study was to assess the effect of antibiotic treatment on the growth and stress level of tobacco (Nicotiana tabacum L.) shoots in vitro as well as the composition of the plant-associated microbiome. The study revealed that shoot cultivation on a medium supplemented with 250 mg L−1 timentin resulted in 29 ± 4% reduced biomass accumulation and a 1.2–1.6-fold higher level of oxidative stress injury compared to the control samples. Moreover, the growth properties of shoots were only partially restored after transfer to a medium without the antibiotic. Microbiome analysis of the shoot samples using multivariable region-based 16S rRNA gene sequencing revealed a diverse microbial community in the control tobacco shoots, including 59 bacterial families; however, it was largely dominated by Mycobacteriaceae. Antibiotic treatment resulted in a decline in microbial diversity (the number of families was reduced 4.5-fold) and increased domination by the Mycobacteriaceae family. These results imply that the diversity of the plant-associated microbiome might represent a significant factor contributing to the efficient propagation of in vitro tissue culture.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enduring Effect of Antibiotic Timentin Treatment on Tobacco In Vitro Shoot Growth and Microbiome Diversity

Tamošiūnė

Andriūnaitė

Vinskienė

et al. 2022

Plants

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“…Plant in vitro propagation techniques based on axillary bud proliferation are often applicable in plant biology research, germplasm preservation and the industrial-scale production of vegetatively propagated plants, such as ornamentals, vegetables and agronomic crops [ 8 , 9 ]. Several studies have shown that endophytic bacteria are common in plant tissues grown in vitro [ 2 , 10 , 11 ] and their composition depends on explant origin and cultivation conditions. Until recently, the composition and role of the endophytic microbiome of in vitro plant organs and tissue cultures attracted little attention and were mainly addressed as contamination resulting from endophytic bacteria overgrowth [ 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have shown that endophytic bacteria are common in plant tissues grown in vitro [ 2 , 10 , 11 ] and their composition depends on explant origin and cultivation conditions. Until recently, the composition and role of the endophytic microbiome of in vitro plant organs and tissue cultures attracted little attention and were mainly addressed as contamination resulting from endophytic bacteria overgrowth [ 11 , 12 , 13 , 14 , 15 ]. However, during the last decade, several studies have shown that bacterial endophytes are common in plant tissues grown in vitro and their beneficial effects on plant culture growth, acclimatization and rooting have been recognized.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Nicotiana tabacum L. In Vitro Shoot Growth by Endophytic Bacillus cereus Group Bacteria

et al. 2021

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In vitro plant tissue cultures face various unfavorable conditions, such as mechanical damage, osmotic shock, and phytohormone imbalance, which can be detrimental to culture viability, growth efficiency, and genetic stability. Recent studies have revealed a presence of diverse endophytic bacteria, suggesting that engineering of the endophytic microbiome of in vitro plant tissues has the potential to improve their acclimatization and growth. Therefore, the aim of this study was to identify cultivated tobacco (Nicotiana tabacum L.) endophytic bacteria isolates that are capable of promoting the biomass accumulation of in vitro tobacco shoots. Forty-five endophytic bacteria isolates were obtained from greenhouse-grown tobacco plant leaves and were assigned to seven Bacillus spp. and one Pseudomonas sp. based on 16S rRNA or genome sequence data. To evaluate the bacterial effect on in vitro plant growth, tobacco shoots were inoculated with 22 isolates selected from distinct taxonomic groups. Four isolates of Bacillus cereus group species B. toyonensis, B. wiedmannii and B. mycoides promoted shoot growth by 11%–21%. Furthermore, a contrasting effect on shoot growth was found among several isolates of the same species, suggesting the presence of strain-specific interaction with the plant host. Comparative analysis of genome assemblies was performed on the two closely related B. toyonensis isolates with contrasting plant growth-modulating properties. This revealed distinct structures of the genomic regions, including a putative enzyme cluster involved in the biosynthesis of linear azol(in)e-containing peptides and polysaccharides. However, the function of these clusters and their significance in plant-promoting activity remains elusive, and the observed contrasting effects on shoot growth are more likely to result from genomic sequence variations leading to differences in metabolic or gene expression activity. The Bacillus spp. isolates with shoot-growth-promoting properties have a potential application in improving the growth of plant tissue cultures in vitro.

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An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives

Loyola-Vargas,

Ochoa-Alejo

2024

Plant Cell Culture Protocols

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Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F.Muell.: a micropropagation approach

Cited by 7 publications

References 58 publications

Enduring Effect of Antibiotic Timentin Treatment on Tobacco In Vitro Shoot Growth and Microbiome Diversity

Enduring Effect of Antibiotic Timentin Treatment on Tobacco In Vitro Shoot Growth and Microbiome Diversity

Stimulation of Nicotiana tabacum L. In Vitro Shoot Growth by Endophytic Bacillus cereus Group Bacteria

An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives

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