Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model

Silva, Emmanuel João Nogueira Leal; Carvalho, Nancy Kudsi; Ronconi, Carina Taboada; De‐Deus, Gustavo; Zuolo, Mário Luis; Zaia, Alexandre Augusto

doi:10.1590/0103-6440201600792

Cited by 20 publications

(17 citation statements)

References 24 publications

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“…8 The unfavorable results of MTA Fillapex in cell culture are reported even when compared to that of resin epoxy-based material such as AH Plus. 30 The results are observed in traditional two-dimensional (2D) and three-dimensional (3D) systems, 31 and are corroborated by results observed in our in vitro evaluation as follows: a greater reduction was observed in the cellular metabolism by MTA Fillapex when compared to that of the Control or AH Plus while using less-diluted extracts. The AH Plus exhibits less cytotoxicity with increases in dilution, and this is in agreement with the results of a previous study, which indicated that a greater dilution of AH Plus after 24 h caused minor cytotoxic effects toward L929 cells.…”

Section: Discussionsupporting

confidence: 80%

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

et al. 2019

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This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric data was analyzed via the Kruskal-Wallis test followed by the Dunn test (p < 0.05). A reduction in cell viability was observed in the extracts that were more diluted for Sealer Plus BC when compared to that of Control and AH Plus (p < 0.05). However, the 1:50 dilution of the Sealer Plus BC was similar to that of the Control (p > 0.05). Conversely, more diluted extracts of MTA Fillapex (1:200) and AH Plus (1:100 and 1:200) were similar to the Control (p > 0.05). Histological analysis performed at 7 d did not indicate any significant difference between tissue response for all materials, and the fibrous capsule was thick (p > 0.05). At 30 d, Sealer Plus BC was similar to the Control (p > 0.05) and MTA Fillapex and AH Plus exhibited greater inflammation than the Control (p < 0.05). The fibrous capsule was thin for the Control and for most specimens of Sealer Plus BC and AH Plus. Thus, Sealer Plus BC is biocompatible when compared to MTA Fillapex and AH Plus, and it is less cytotoxic when less-diluted extracts are used.

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Section: Discussionsupporting

confidence: 80%

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

et al. 2019

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“…In this study, the cell viability induced by MTA-HP was lower than that of its precursor in some dilutions during the initial periods; however, it had a better result compared to white MTA-Ang when the former was more diluted. It should be noted that in vitro studies represent the first level of research and are important for understanding biological risks before using these materials in vivo (16). However, tests with cell cultures have limitations, especially when using two-dimensional cell cultures, as in this study (8,16).…”

Section: Discussionmentioning

confidence: 94%

“…It should be noted that in vitro studies represent the first level of research and are important for understanding biological risks before using these materials in vivo (16). However, tests with cell cultures have limitations, especially when using two-dimensional cell cultures, as in this study (8,16).…”

Section: Discussionmentioning

confidence: 94%

Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material

et al. 2019

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New mineral trioxide aggregate (MTA) formulations are constantly introduced in the market, usually in a powder-and-liquid form. Bioceramic (Bio-C) Repair is a ready-for-use material suggested as substitute for MTA, but its properties need to be studied. This study evaluated the cytotoxicity, biocompatibility and biomineralization of Bio-C Repair compared to MTA Repair High-Plasticity (MTA-HP) and white MTA-Angelus (MTA-Ang). L929 fibroblasts were exposed to material-extracted (undiluted, ½ and ¼ dilutions; 6, 24 and 48h). Polyethylene tubes with material or empty (control) were implanted in the subcutaneous tissue of rats. After 7 and 30 days (n=8), the specimens were removed for analysis (hematoxylin-eosin, von Kossa and polarized light). Cytotoxicity data were statistically analyzed by two-way ANOVA, and biocompatibility data by Kruskal-Wallis and Dunn tests (p<0.05). The cells exposed to the materials had greater viability at most of the periods compared with control (p<0.05). The undiluted and ½ dilutions of MTA-HP extract showed higher cytocompatibility than Bio-C Repair at 6 h and with the ¼ dilution at 24 h (p<0.05); the white MTA-Ang showed higher cytocompatibility than Bio-C Repair at most of periods (p<0.05). The undiluted white MTA-Ang extract had higher cytocompatibility at 6 and 24h than MTA-HP, and with ½ dilution at 24h (p<0.05). The materials’ cytocompatibility was similar at 48h for most dilutions (p>0.05). At 7 and 30 days, the groups had moderate and mild inflammation, respectively (p>0.05). All materials showed positive structures for von Kossa and polarized light. In conclusion, Bio-C Repair had similar cytocompatibility to MTA-based materials is biocompatible and induces biomineralization.

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“…It was created combining a material with excellent biocompatibility and bioactive potential such as MTA with synthetic resins with good physical properties. However, this blend has shown cytotoxicity [4,5]. The Brasseler Industry (Savannah, GA, USA) introduced the EndoSequence Root Repair Material; a premixed bioceramic material indicates to pulp capping/pulpotomy, perforation repair, apexification, and root-end filling.…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers

et al. 2020

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Objectives: This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus. Materials and Methods: BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethylthiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). Results: The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05). Conclusions: All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

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Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model

Cited by 20 publications

References 24 publications

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material

Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers

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