Continuous therapeutic ultrasound in the healing process in rat skin

Carrer, Viviani de Marque; Setti, João Antônio Palma; Veronez, Djanira da Luz; Moser, Auristela Duarte de Lima

doi:10.1590/0103-5150.028.004.ao12

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…That said, the cellular reduction in the G3 continuous group may also be added to the thermal effect of the TUS caused by the mechanical energy transformation of its uninterrupted pulses through the attenuation by the tissue layers. (25)(26)(27) Differently from Maeshige et al (28) who reported on the increase in the amount of proteins and mRNA expressed by mechanically stimulated dermal fibroblasts by TUS (0.1; 0.5 or 1 W/cm 2 e 20%), at 24 and 48 hours. In parallel, human fibroblasts showed increase in cell proliferation tests with 5, 15, 30 and 50 mW/ and pulse in 30%.…”

Section: Discussionmentioning

confidence: 89%

Effect of therapeutic ultrasound of low intensity pulsed and continuous in fibroblastic cells in vitro

Oliveira¹,

Pires-Oliveira²,

Pacheco‐Soares³

et al. 2020

mtprehabjournal

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Introduction: The ultrasound is widely used clinical practice focused on tissue repair, because it is a secure resource, minimally-invasive and low investment. However, despite the effects of ultrasound have been well described, the ideal relationship between dose and effect, still needs to be better elucidated and bounded. Objective: In this way, the objective of this study was to evaluate the influence of irradiation pulsed ultrasound and continuous in the viability of fibroblastic cells L929 in vitro. Method: The cells were distributed in TPP plates 12 wells in the concentration of 5x10 5 cells/µL and subdivided into the following groups: G1: control (not received radiation), G2: irradiated to 0.5 W/cm 2-30% and G3: irradiated to 0.5 W/cm 2-100%. Then received ultrasonic irradiation with intervals of 24, 48 and 72 hours, and after 24 hours of each irradiation was performed MTT cytotoxicity cell. Results: Among the three groups analyzed, only the G2 group showed a significant difference between the time 48 and 72 hours (p=0.05). In other times, despite variation in the percentage of viable cells, were not significant. When compared to peers at post-test, it was possible to observe a difference of 29% of viable cells between the groups G1 and G2 (p=0.05). However, the biggest difference was observed between the groups G2 and G3 (p=0.05). Conclusion: In this way, it can be concluded that irradiation pulsed ultrasound showed higher proliferation of fibroblasts L929 in time 48 hours, whereas in group irradiated in continuous mode, there was no statistically significant difference between the times.

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Section: Discussionmentioning

confidence: 89%

Effect of therapeutic ultrasound of low intensity pulsed and continuous in fibroblastic cells in vitro

Oliveira¹,

Pires-Oliveira²,

Pacheco‐Soares³

et al. 2020

mtprehabjournal

View full text Add to dashboard Cite

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“…19 On the contrary, HIFU is known to reduce these reactions, which improved the quality and normality of inflammatory reactions. [20][21][22] Although few inflammatory cells and multinuclear giant cells were detected, a previous study demonstrated that native collagen matrix and dehydrothermally cross-linked matrix were associated with multinucleated cellular infiltration observed between the collagen bundles. The study reported that a native porcine collagen matrix seems to be the best option for soft tissue augmentation procedures even if the volume stability is lower than with non-crosslinked collagen.…”

Section: Discussionmentioning

confidence: 89%

A Novel Acellular Dermal Scaffold Prepared Using High-Intensity Focused Ultrasound Energy for the Repair of Soft Tissue Defects

Abdul-Monem

Helal

Aboushelib

2020

European Dental Research and Biomaterials Journal

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Objective To evaluate a naturally derived acellular dermal scaffold for soft tissue reconstruction using high-intensity focused ultrasound energy (HIFU). Materials and Methods Acellular dermal scaffolds (ADSs) were prepared by purification of bovine skin. Half of the scaffolds were subjected to high-intensity focused ultrasound energy (HIFU) to modify collagen structure, whereas the other half was used as control. A large skin defect was made in the dorsum of white mice, and the scaffolds were used to cover the induced defects. Wound healing was evaluated histologically after 2, 6, and 12 weeks using common and specific stained sections (n = 20). Statistical Analysis Mean values and standard deviations were calculated for each group, and Student’s t-test was used for statistical analysis (α= 0.05; n = 20). Results After 2 weeks, all examined specimens revealed the presence of inflammatory cellular infiltration and early immature blood vessel formation. After 6 weeks, inflammatory cellular infiltration was reduced, with evidence of maturation of new blood vessels observed for all groups. After 12 weeks, there was a significant increase (F = 124, p < 0.01) in new collagen formation and count of mature blood vessels observed for the HIFU group compared with control. Evidence of remodeling of new collagen fibers and biodegradation of the grafts was also observed. Conclusions HIFU-modified ADSs enhanced wound healing and could be used to cover large soft tissue defects.

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“…Increasing quantity of collagen types I and III indicates the success of the healing process in the remodeling phase (21 days). 25 Alkahtani, et al stated that low-intensity ultrasonic therapy doses between 0.5-3 W/cm 2 provide the best results and minimal side effects, and are most effective given in the first and second phases of the wound healing process. Low-intensity ultrasonic therapy can promote healing of open wounds as well as deep tissue wounds.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-intensity pulsed ultrasound on mast cell degranulation and fibroblast expression on type 2 diabetes mellitus rats wound healing process

Maharani

Hasan

Dwirahardjo

et al. 2022

Majalah Kedokteran Gigi Indonesia

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Impaired wound healing is one of the Diabetes mellitus complications. Low-intensity Pulsed Ultrasound (LIPUS) therapy may accelerate the impaired wound healing. The use of LIPUS therapy in the early inflammatory phase can induce mast cell degranulation, and in the proliferative phase it can increase collagen synthesis by fibroblasts. The purpose of this study was to determine the effects of LIPUS therapy on mast cell degranulation and fibroblastexpression in the healing process of punch biopsy wound in rats with type 2 diabetes mellitus. Twenty-four Sprague dawley (n=24) were designed into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then divided into six groups: diabetes mellitus without LIPUS (DM3, DM7, DM14) and diabetes mellitus with LIPUS (DML3, DML7, DML14), 4 each, and punch biopsy wounds were made on the dorsal skin. The DML group received LIPUS therapy in the wound area (frequency 3 MHz, intensity 0.5 W/cm2, duty cycle 20%, duration 3 minutes every day for 3 days (DML3), 7 days (DML7), and 14 days (DML14). The wounded tissue area was stained with toluidine blue to observe mast cell degranulation and immunohistochemical type HSP-47 to observe fibroblast expression. Two-Way ANOVA and Post Hoc LSD tests were used to determine the differences in mast cell degranulation and fibroblast expression. The results showed that mast cell degranulation and fibroblast expression in the DML group were higher than in the DM group (table 1). Pearson test showed a correlation between mast cell degranulation and fibroblast expression (p=0.00; r= 0.839). LIPUS therapy increases mast cell degranulation and fibroblast expression in type 2 diabetes mellitus rat model. The higher the mast cell degranulation, the higher fibroblast expressions.

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Continuous therapeutic ultrasound in the healing process in rat skin

Cited by 7 publications

References 18 publications

Effect of therapeutic ultrasound of low intensity pulsed and continuous in fibroblastic cells in vitro

Effect of therapeutic ultrasound of low intensity pulsed and continuous in fibroblastic cells in vitro

A Novel Acellular Dermal Scaffold Prepared Using High-Intensity Focused Ultrasound Energy for the Repair of Soft Tissue Defects

Effect of low-intensity pulsed ultrasound on mast cell degranulation and fibroblast expression on type 2 diabetes mellitus rats wound healing process

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