Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Padilha, João Henrique Delfrate; Ribas, Luciana Lopes Fortes; Amano, Érika; Quoirin, Marguerite

doi:10.1590/0102-33062015abb0109

Cited by 12 publications

(8 citation statements)

References 45 publications

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“…Due to its attractive features, the macaw palm domestication, genetic breeding and development of commercial plantations have encouraged financial investments in basic research, biotechnology and industry. Studies in several areas have been carried out with this purpose, including ecophysiology (Pires et al 2013), mating system (Lanes et al 2016), vegetative development (Berton et al 2013;Machado et al 2016), fruit development (Montoya et al 2016), phenotypic diversity (Ciconini et al 2013;Lanes et al 2015;Conceição et al 2015;Coser et al 2016) and biotechnological applications (Moura et al 2009;Luis and Scherwinski-Pereira 2014;Padilha et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Plastome sequences are also of great importance to understand evolutionary events in plants based on the knowledge of gene content, recombination events, loss of genes, gene transfer to the nucleus and genome rearrangements (Vieira et al 2016b;Bock 2017;Lopes et al 2017;Park et al 2017;Ruhlman et al 2017), as well as for plastid transformation aiming basic research (Rogalski et al 2006(Rogalski et al , 2008Alkatib et al 2012) and biotechnological applications (Daniell et al 2016;Zhang et al 2017) since the complete sequence of plastome is a prerequisite to choose target intergenic regions for insertion of transgenes and development of transplastomic plants (Daniell et al 2016;Fuentes et al 2017). The plastid transformation has been used efficiently for metabolic engineering (Daniell et al 2016;Fuentes et al 2017) and it is a viable alternative to manipulate plastid fatty acid biosynthesis (Rogalski and Carrer 2011) given that several efficient protocols of plant regeneration based on somatic embryogenesis are available for macaw palm (Moura et al 2009;Luis and Scherwinski-Pereira 2014;Padilha et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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The complete plastome of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] and extensive molecular analyses of the evolution of plastid genes in Arecaceae

Lopes

Pacheco

Nimz

et al. 2018

Planta

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The plastome of macaw palm was sequenced allowing analyses of evolution and molecular markers. Additionally, we demonstrated that more than half of plastid protein-coding genes in Arecaceae underwent positive selection. Macaw palm is a native species from tropical and subtropical Americas. It shows high production of oil per hectare reaching up to 70% of oil content in fruits and an interesting plasticity to grow in different ecosystems. Its domestication and breeding are still in the beginning, which makes the development of molecular markers essential to assess natural populations and germplasm collections. Therefore, we sequenced and characterized in detail the plastome of macaw palm. A total of 221 SSR loci were identified in the plastome of macaw palm. Additionally, eight polymorphism hotspots were characterized at level of subfamily and tribe. Moreover, several events of gain and loss of RNA editing sites were found within the subfamily Arecoideae. Aiming to uncover evolutionary events in Arecaceae, we also analyzed extensively the evolution of plastid genes. The analyses show that highly divergent genes seem to evolve in a species-specific manner, suggesting that gene degeneration events may be occurring within Arecaceae at the level of genus or species. Unexpectedly, we found that more than half of plastid protein-coding genes are under positive selection, including genes for photosynthesis, gene expression machinery and other essential plastid functions. Furthermore, we performed a phylogenomic analysis using whole plastomes of 40 taxa, representing all subfamilies of Arecaceae, which placed the macaw palm within the tribe Cocoseae. Finally, the data showed here are important for genetic studies in macaw palm and provide new insights into the evolution of plastid genes and environmental adaptation in Arecaceae.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The complete plastome of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] and extensive molecular analyses of the evolution of plastid genes in Arecaceae

Lopes

Pacheco

Nimz

et al. 2018

Planta

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“…Similar results were obtained for E. guineensis genotypes BRS C2328: On MS medium containing 0.6 µM ANA and 12.3 µM 2iP, 48% of the calli were embryogenic and 52.5% of them produced SEs (Silva et al, 2012). Likewise, in Acrocomia aculeata, Padilha et al (2015) observed friable calli in 53% of TCLs cultivated in Y3 culture medium supplemented with 75 µM Picloram and 12.5 µM 2iP. These results show the importance of auxin/cytokinin balance for embryogenic callus multiplication.…”

Section: Discussionmentioning

confidence: 62%

“…This technique has been used successfully for peach palm (Bactris gasipaes) (Steinmacher et al, 2007), Acrocomia aculeate (Padilha et al, 2015) and oil palm (E. guineensis) (Scherwinski-Pereira et al, 2010). This study was developed to address the need for multiplication and production of hybrid oil palm BRS Manicoré, aimed at more rapid production on a scale that meets national demand.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

Bonetti¹,

Nesi²,

Quisen³

et al. 2016

Afr. J. Biotechnol.

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Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were compared (Y3 and modified MS), and several growth regulators (2,4-D, Picloram, BA, 2iP), putrescine and activated charcoal (AC) were used during the different stages. Somatic embryos (SE) were obtained from ZE-derived embryogenic masses cultured on Y3 medium with or without 2,4-D (9 µM) + 1000 µM putrescine and 40% were converted into seedlings in the Y3 medium supplemented with 2 g.L -¹ AC and without growth regulators, under light. SE developed in 49 to 53% TCL-derived calli and 50% of them were converted into seedlings in the same conditions described for ZE calli. The yield of both processes was compared, showing the superiority of TCL explants for SE production.

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“…picloram associated with BAP or 2iP also induced the formation of embryogenic masses (Padilha et al, 2015).…”

Section: Discussionmentioning

confidence: 93%

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)

et al. 2019

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Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root explants of oil palm (Elaeis guineensis Jacq). Apex root explants of approximately 0.5 cm were isolated from plants cultivated in vitro and inoculated in Y3 culture medium in the following treatments: A - without growth regulators; B - 1 mg.L-1 picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); C - 1 mg.L-1 picloram and 2 mg.L-1 2ip (2-isopentenyladenine); D - 2 mg.L-1 2ip; E - 1 mg.L-1 picloram and 2 mg.L-1 BAP (6-benzylaminopurine); F - 2 mg.L-1 BAP; and G - 14.5 mg.L-1 spermine. After six months of culturing, the calli induced in the treatments were analyzed by light microscopy. The calli induced in the treatments with 1 mg.L-1picloram (B) and treatment with 14.5 mg.L-1spermine (G) exhibited embryogenic characteristics, small and isodiametric cells, forming agglomerates, besides a large amount of starch. Calli of the best treatment (Y3 com 1 mg.L-1 de picloram) were inoculated in Y3 culture medium without addition of growth regulators. After eight months, calli were once more analyzed under light microscopy. All the treatments showed callus formation, except for treatments D and A. Calli of treatment B exhibited cells with embryogenic characteristics that developed somatic embryos.

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Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Cited by 12 publications

References 45 publications

The complete plastome of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] and extensive molecular analyses of the evolution of plastid genes in Arecaceae

The complete plastome of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.] and extensive molecular analyses of the evolution of plastid genes in Arecaceae

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)

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