Optimal culture conditions for the initial development of Ilex paraguariensis A.St.-Hil. explants

Abstract

In the state of Rio Grande do Sul, Brazil, yerba mate (Ilex paraguariensis A. St.-Hil.) is an economically and culturally important species. Because of the difficulty in obtaining plantlets from seeds, micropropagation has emerged as an alternative that could increase production. This work aimed to evaluate the effect that treatment with 6-benzyladenine (BA), at concentrations of 2.2, 8.9, and 20 μM and associated with naphthaleneacetic acid (NAA, 0.5 μM), has on the in vitro growth and leaf emission of I. paraguariensis nodal segments cultivated in 0.5 X Murashige and Skoog (MS) medium or 0.5 X Woody Plant Medium (WPM). The most efficient treatment was MS medium with 8.9 μM BA+NAA, in which I. paraguariensis presented greater shoot elongation and higher leaf counts than in the other treatments. Therefore, use of the MS medium with 8.9 μM BA+NAA is recommended in order to increase efficiency in the initial development of I. paraguariensis explants.

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

“…PGRs are important at all stages of plant tissue culture (Bidabadi and Jain, 2020) and are added to stimulate the formation of primordial roots in tissues presenting a rooting predisposition (Griebeler et al, 2014). In this study, BA had the most apparent effect on the induction and proliferation of C. hystrix shoots, confirming that BA plays a significant role in tissue culture (Namli et al, 2010).…”

“…Plants were cultivated in a greenhouse and regularly sprayed with 0.1% carbendazim. This approach has also been reported in other studies (Griebeler et al, 2014). The combination of Tween 80 and HgCl 2 can also improve sterilization, and its superiority has been reported in previous studies (San-José et al, 2010;Martínez et al, 2017a).…”

Establecimiento in vitro de Yerba mate (Ilex paraguariensis A. St.-Hil.) nativa de Uruguay

“…En otras especies se ha demostrado que la capacidad de propagación está controlada genéticamente y que existe variabilidad en las poblaciones naturales (Beyl y Trigiano, 2015). En Argentina y Brasil, existen numerosos trabajos publicados en los que se han evaluado diferentes alternativas para propagar vegetativamente plantas de yerba mate (Salas Pino y Laviosa, 1998;Sansberro et al, 1999Sansberro, Mroginski y Bottini, 2000Wendling, 2004;Tarragó et al, 2005Tarragó et al, , 2012Dolce y Rey, 2006;Wendling et al, 2007Wendling et al, , 2013Dutra, Hansel y Wen-dling, 2008;Horbach, 2008;Brondani et al, 2010;Dolce, Mroginski y Rey, 2010;Griebeler et al, 2014). Sin embargo, hasta el momento existen muy pocos estudios sobre las poblaciones silvestres de yerba mate uruguayas.…”