In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria

Tomasi, Jéssica de Cássia; Degenhardt, Juliana; Grunennvaldt, Renata Lúcia; Santos, Germana Davila dos; Vicente, Vânia A.; Franciscon, Luziane; Bona, Cleusa; Quoirin, Marguerite

doi:10.1007/s11676-018-0763-x

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…Previous studies were able to reduce oxidation with AC supplementation, a carbonaceous adsorbent of aromatic compounds (de Cássia Tomasi et al 2019). Similar results were observed in this study, and 2 g l -1 of AC in the growing media significantly reduced oxidation up to 90%.…”

Section: Discussionsupporting

confidence: 91%

In vitro propagation of the Amazonian medicinal plant guayusa (Ilex guayusa) and effects of light in the growth and development of this shade tolerant plant

Carvalho

Ortega

Orellana

et al. 2021

Plant Cell Tiss Organ Cult

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Guayusa (Ilex guayusa) is an endemic plant from the Amazon with potential medicinal applications. Indigenous people are familiar with such applications and use guayusa based on ancestral knowledge. There is a growing interest in guayusa-based products in urban areas of Ecuador and internationally. The supply cannot meet the demand. Currently, traditional practices are used for guayusa growth and the potential use of the protected forest is foreseen. This work describes a protocol for the in vitro propagation of guayusa, a sustainable solution to generate high quality plants in reduced space. Stakes obtained from stems were used as explants. Chemical sterilization with ethanol and sodium hypochlorite resulted in 100% surface-sterilized stakes. The growth medium mWPM resulted in favorable outcomes regarding shoot development and elongation, as well as rooting.Supplementation with activated charcoal resulted in reduced browning during the elongation phase. The majority of shoots were able to develop roots spontaneously. Medium supplementation with the auxin indole-3-butyric acid, IBA, may be considered when rooting does not occur spontaneously. Acclimatization was performed in soil.The protocol was tested under different light spectra, revealing that guayusa growth is affected by light quality.The photobiology of this shade tolerant plant requires further characterization, but the data uncovered a potential role for green and far-red light in root development. Key messageGuayusa was propagated on mWPM medium supplemented with activated charcoal. Spontaneous root development occurred in most shoots. Light quality affected plant development, green and far-red light could influence root growth.

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Section: Discussionsupporting

confidence: 91%

In vitro propagation of the Amazonian medicinal plant guayusa (Ilex guayusa) and effects of light in the growth and development of this shade tolerant plant

Carvalho

Ortega

Orellana

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

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DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

Santos

Cezario

Simões

et al. 2020

CERNE

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In Vitro Propagation of the Amazonian Medicinal Plant Guayusa (Ilex Guayusa) and Effects of Light in the Growth and Development of this Shade Tolerant Plant

Carvalho

Ortega

Orellana

et al. 2021

Preprint

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Guayusa ( Ilex guayusa ) is an endemic plant from the Amazon with potential medicinal applications. Indigenous people are familiar with such applications and use guayusa based on ancestral knowledge. There is a growing interest in guayusa-based products in urban areas of Ecuador and internationally. The supply cannot meet the demand. Currently, traditional practices are used for guayusa growth and the potential use of the protected forest is foreseen. This work describes a protocol for the in vitro propagation of guayusa, a sustainable solution to generate high quality plants in reduced space. Stakes obtained from stems were used as explants. Chemical sterilization with ethanol and sodium hypochlorite resulted in 100% surface-sterilized stakes. The growth medium mWPM resulted in favorable outcomes regarding shoot development and elongation, as well as rooting. Supplementation with activated charcoal resulted in reduced browning during the elongation phase. The majority of shoots were able to develop roots spontaneously. Medium supplementation with the auxin indole-3-butyric acid, IBA, may be considered when rooting does not occur spontaneously. Acclimatization was performed in soil. The protocol was tested under different light spectra, revealing that guayusa growth is affected by light quality. The photobiology of this shade tolerant plant requires further characterization, but the data uncovered a potential role for green and far-red light in root development.

show abstract

In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria

Cited by 3 publications

References 52 publications

In vitro propagation of the Amazonian medicinal plant guayusa (Ilex guayusa) and effects of light in the growth and development of this shade tolerant plant

In vitro propagation of the Amazonian medicinal plant guayusa (Ilex guayusa) and effects of light in the growth and development of this shade tolerant plant

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

In Vitro Propagation of the Amazonian Medicinal Plant Guayusa (Ilex Guayusa) and Effects of Light in the Growth and Development of this Shade Tolerant Plant

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