Evaluation of liver regeneration diet supplemented with omega-3 fatty acids: experimental study in rats

Abstract

Evaluation of liver regeneration diet supplemented with omega-3 fatty acids: experimental study in rats 393Rev. Col. Bras. Cir. 2015; 42(6): [393][394][395][396][397] Original Article Original Article Original Article Original Article Original Article DOI: 10.1590/0100-69912015006008 Evaluation of liver regeneration diet supplemented with omega-3 Evaluation of liver regeneration diet supplemented with omega-3 Evaluation of liver regeneration diet supplemented with omega-3 Evaluation of liver regeneration diet supplemented with omega-3 Evaluation of liver regeneration diet supplemented with omega-3 fatty acids: experimental study in rats fatty acids: experimental study in rats fatty acids: experimental study in rats fatty acids: experimental study in rats fatty acids: experimental study in rats Avaliação da regeneração hepática com dieta suplementada com ácidos graxos Avaliação da regeneração hepática com dieta suplementada com ácidos graxos Avaliação da regeneração hepática com dieta suplementada com ácidos graxos Avaliação da regeneração hepática com dieta suplementada com ácidos graxos Avaliação da regeneração hepática com dieta suplementada com ácidos graxos ômega-3: estudo experimental em ratos ômega-3: estudo experimental em ratos ômega-3: estudo experimental em ratos ômega-3: estudo experimental em ratos ômega-3: estudo experimental em ratos Objective Objective: to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study. Methods Methods Methods Methods Methods: thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gammaglutamyltranspeptidase, and mitosis of the liver stained with H&E. Results Results ResultsResults Results: the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups. Conclusion Conclusion Conclusion Conclusion Conclusion: supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.

Current Knowledge about the Effect of Nutritional Status, Supplemented Nutrition Diet, and Gut Microbiota on Hepatic Ischemia-Reperfusion and Regeneration in Liver Surgery

“…The preoperative nutritional state considerably affects postoperative metabolism, organ function, and inflammatory responses [17], and nutritional status affects the liver regenerative capacity [18]. Therefore, the basal alimentary condition of the patient plays an important role in predicting postoperative complications.…”

“…Conversely, several dietary components significantly benefit health [56], presenting antioxidant or anti-inflammatory properties as well as contributing to modifying the gut microbiome [18]. As a result, the re-establishment and maintenance of the correct nutritional status by these nutraceuticals and functional foods before, during, and/or after surgery could lead to improvements in complications related to I/R injury, representing a potential approach alone or in combination with other therapies to improve patient outcomes.…”

“…In experimental and clinical studies performed in animals and humans, fatty acids, arginine, and nucleotides have shown the ability to modulate immune and inflammatory responses [18,69]. These nutrients, among others, have been labeled as pharmaconutrients [18].…”

“…Recent advances suggest new concerns about the pathophysiology of hepatic I/R injury. Preexisting nutritional status might affect the post-operative metabolism, liver function, inflammation, and regenerative capacity [17,18]. Starvation exacerbates warm ischemic injury due to the amount of glycogen stored in the liver [19][20][21][22].…”

Omega-3 fatty acids inhibit oxidative stress in a rat model of liver regeneration

“…Omega-3 fatty acids (Ω3FAs) and their longer chain downstream products such as eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) have been demonstrated as beneficial on the liver in several different aspects [456]. However, the number of studies focusing on the effects of Ω3FAs on liver regeneration is relatively few with different results [78]. Moreover, the effects of adding these emulsions to total parenteral nutrition (TPN) on the oxidative stress, which develop in the regenerating liver, are not clear.…”