Terapia fotodinâmica no controle de Xanthomonas campestris pv. campestris in vitro e no tratamento de sementes de canola naturalmente contaminadas

Zancan, Nayara Lima Baute; Tebaldi, Nilvanira Donizete

doi:10.1590/0100-5405/199134

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…In addition, combination of MB plus TB in the clear phase eliminated the bacterial growth of Xanthomonas campestris pv. campestris found in naturally contaminated canola seeds, which did not occur when the photosensitizers were tested alone [45]. For treatment of OSCC, this is the first report related to the synergic effect of a combination of MB plus TB in the dark phase and clear phase.…”

Section: Methylene Blue Incorporated (%) Methylene Blue Incorporated (%)mentioning

confidence: 81%

Photodynamic Therapy with an Association of Methylene Blue and Toluidine Blue Promoted a Synergic Effect against Oral Squamous Cell Carcinoma

Rocha,

Bomfim,

Junior

et al. 2023

Cancers

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Among the most malignant cancers, oral squamous cell carcinoma (OSCC) stands out as the most common malignant head and neck tumor. Despite advances in the field of treatment, the prognosis of patients with OSCC remains poor. Aiming to overcome the limitations of the currently existing therapies against OSCC, the present work aims to investigate the potential of photodynamic therapy (PDT) with phenothiazine derivatives used alone or in combination. The incorporation of methylene blue (MB) and toluidine blue (TB) was evaluated in OSCC cell lines (HSC-3 and SCC-9) and a nontumor cell line (Hfib). Both compounds exhibited concentration and time-dependent incorporation, with higher rates observed in tumor cells. Regarding dark-phase cytotoxic activity, SCC-9 cells were the most sensitive cell line with an IC50 value of 362.6 µM and 41.4 µM for MB and TB, respectively. Using PDT, all lineages showed greater sensitivity, presenting lower IC50 values when compared to the dark phase values. The combination index values of 0.69 (dark phase) and 0.73 (clear phase) associated with concave isobolograms, in both phases, revealed that MB and TB have synergistic effects when combined against SCC-9 cells. These findings suggest that MB or TB assisted with PDT holds promise for OSCC treatment.

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Section: Methylene Blue Incorporated (%) Methylene Blue Incorporated (%)mentioning

confidence: 81%

Photodynamic Therapy with an Association of Methylene Blue and Toluidine Blue Promoted a Synergic Effect against Oral Squamous Cell Carcinoma

Rocha,

Bomfim,

Junior

et al. 2023

Cancers

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Nanoparticles and photodynamic therapy in the treatment and control of Alternaria alternata in wheat seeds

2022

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The expansion of wheat crops (Triticum spp.) to the Brazilian Cerrado highlights the need to use pathogen-free seeds. This study aimed at evaluating the efficacy of nanoparticles (NPs) and photodynamic therapy (PhT) in inhibiting the in vitro growth of the fungus Alternaria alternata, in its treatment and control in naturally contaminated wheat seeds, and in the physiological quality of the seeds. The efficacy of NPs (ZnOCl, ZnOCl:1Cu, ZnOCl:0.1Ag; ZnO:1Cu, ZnO, and ZnO:1Ag) and PhT using methylene blue (MB) and toluidine blue (TB) dyes was evaluated in inhibiting the mycelial growth of A. alternata and in the treatment and control of the pathogen in wheat seeds by evaluating germination, emergence, GSI, ESI, accelerated ageing, and health. All NPs at 2.5 and 5 mg mL-1 concentrations and the dyes MB, TB, MB + TB at 50 and 100 µmol L-1 inhibited mycelial growth and reduced the incidence of A. alternata in the seeds. The NP ZnO:1Ag at 5 mg mL-1 and the MB + TB dye at 100 µmol L-1 were the most effective in inhibiting mycelial growth. NPs and PhT did not affect the physiological quality of seeds and controlled A. alternata in wheat seeds, demonstrating potential use in the treatment and control of the pathogen in wheat seeds.

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Terapia fotodinâmica no controle de Xanthomonas campestris pv. campestris in vitro e no tratamento de sementes de canola naturalmente contaminadas

Cited by 2 publications

References 20 publications

Photodynamic Therapy with an Association of Methylene Blue and Toluidine Blue Promoted a Synergic Effect against Oral Squamous Cell Carcinoma

Photodynamic Therapy with an Association of Methylene Blue and Toluidine Blue Promoted a Synergic Effect against Oral Squamous Cell Carcinoma

Nanoparticles and photodynamic therapy in the treatment and control of Alternaria alternata in wheat seeds

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