Phenolic Composition and Antioxidant Capacity of Aqueous and Ethanolic Extracts of Blackberries

Abstract

-the objective of this study was to evaluate the content of phenolic compounds, anthocyanins and flavonoids extracted with 80% ethanol and water, and antioxidant capacity (DPPH and ABTS) of five blackberry cultivars produced in Western paraná, brazil. brazos, tupy, arapaho, choctaw and Guarani blackberries were studied. Soluble solids, titratable acidity and ascorbic acid of fruits were also characterized. Total phenolic compounds ranged from 8.23 to 14.98 mg GAE g -1 (f.w.) and Arapaho cultivar exhibited the highest contents in both solvents. anthocyanins ranged from 2.57 to 9.42 mg ecy3glu g -1 (f.w.), being the solvent ethanol 80% more efficient than the water, and Choctaw cultivar showing the highest content. Flavonoids ranged from 0.46 to 1.14 mg eQ g -1 (f.w.) and Guarani cultivar showed the highest content. High linear correlations were found between total phenolics and antioxidant capacity in both extraction solvents. however, linear correlations between anthocyanins and antioxidant capacity were observed only for the aqueous solvent. Ascorbic acid ranged from 87.87 to 134.09 mg 100 g -1 , with emphasis on brazos cultivar. the aqueous extract showed greater ability to scavenge abtS radical, but the ethanolic extract was more efficient for the DPPH radical. Blackberries produced under Western Paraná conditions showed high levels of antioxidants with emphasis on Arapaho cultivar. This study showed that water and ethanol solvents influence different results on phenolic composition and antioxidant activity of blackberries. index terms: Rubus spp., total phenolic, flavonoids, anthocyanins.comPosição fenólica e atividade antioxidante dos extratos aquoso e etanólico de amora-Preta resumo -O objetivo deste trabalho foi avaliar o teor de compostos fenólicos, antocianinas e flavonoides, extraídos com etanol a 80% e água, e a capacidade antioxidante (DPPH e ABTS) de cinco cultivares de amora-preta produzidas na região oeste do paraná, brasil. as amoras-pretas 'brazos', 'tupy', 'arapaho', 'choctaw' e 'Guarani' foram estudadas. Sólidos solúveis, acidez titulável e ácido ascórbico também foram caracterizados. Compostos fenólicos totais variaram de 8,23 a 14,98 mg EAG g -1 (p.f.) e 'Arapaho' exibiu os maiores conteúdos em ambos os solventes. antocianinas variaram de 2,57 a 9,42 mg ecy3glu g -1 (p.f.), sendo etanol 80% o extrator mais eficiente, com o maior conteúdo encontrado em 'Choctaw'. Flavonoides variaram de 0,46 a 1,14 eQ g -1 (p.f.) e 'Guarani' mostrou o maior conteúdo. Correlações lineares elevadas foram verificadas entre compostos fenólicos totais e atividade antioxidante em ambos os solventes de extração. O conteúdo de antocianinas totais se correlacionou linearmente com atividade antioxidante apenas quando extraída com solvente aquoso. Ácido ascórbico variou de 87,87 a 134,09 mg 100 g -1 (p.f.), com destaque para 'brazos'. o extrato aquoso apresentou maior capacidade de sequestrar o radical abtS, mas o etanólico foi mais eficiente para o radical DPPH. As amoras-pretas produzidas nas condições do oes...

Investigation on High-Value Bioactive Compounds and Antioxidant Properties of Blackberries and Their Fractions Obtained by Home-Scale Juice Processing

“…Our results are in agreement with the data reported by other studies [37,[43][44][45]. In this regard, the TPC of wild blackberry byproducts revealed by Jazic et al [37] was 5016 mg GAE/100 g d.s.…”

Aqueous extract of berry (Plinia jaboticaba)byproduct modulates gut microbiota and maintains the balance on antioxidant defense system in rats

“…TPC and DPPH in JAE were higher than other work with aqueous extracts of 20 different plants (Wong, Leong, & William Koh, 2006). TPC and yellow flavonoids values found in a study on the aqueous extract of blackberries by Celant, Braga, Vorpagel, and Salibe (2016) were approximately 2.5 and 3 times lower, respectively, than for JAE, in this study. Carotenoids contribute to the lipophilic antioxidant effect of the food matrix.…”

Antioxidant Properties of “Natchez” and “Triple Crown” Blackberries Using Korean Traditional Winemaking Techniques

“…Observed values ranged from 0.350 to 0.420% MA. Our titratable acidity range was similar to the range measured in previous research, from 0.33 to 0.41% MA [8]. Most researchers have recorded somewhat higher titratable acidity values than those observed in the current study [24, 26, 27, 31].…”