Tamanho Ótimo De Amostras De Frutos E De Sementes Para Determinação Da Poliembrionia Em Citros

Santos, Catiane Queiroz De Jesus; Girardi, Eduardo Augusto; Vieira, Elvis Lima; Lêdo, Carlos Alberto da Silva; Filho, Walter dos Santos Soares

doi:10.1590/0100-2945-063/14

Cited by 11 publications

(11 citation statements)

References 11 publications

(12 reference statements)

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“…Since the explants came from individuals obtained from germination of seeds, was determined the polyembryony rates of the seeds of the different genotypes studied, using a sample of 23 seeds of each individual, considering polyembryonic those with more than one embryo per seed, according to methodology described Santos, Girardi, Vieira, Ledo, and Soares Filho (2015).…”

Section: Methodsmentioning

confidence: 99%

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Santiago¹,

Santos²,

Lêdo³

et al. 2019

JAS

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Micropropagation is a method that enables in vitro cloning of cultivated plants, irrespective of their potential to produce seeds and their polyembryony rate, guaranteeing genetic identity and absence of phytosanitary problems of the propagated plants if the multiplication process is conducted suitably. In this respect, citrus rootstocks of agronomic interest were micropropagated in Wood Plant Medium (WPM). The experiment was conducted in the municipality of Cruz das Almas, in the Recôncavo Baiano region of Bahia state, Brazil, with 10 rootstocks: ‘Indio’, ‘Riverside’ and ‘San Diego’ citrandarins, ‘Sunki Tropical’ mandarin, and the hybrids RL × TR-001, FRL × (RL × TR)-005, CSM × (RL × TR)-059, TRH-051, TRH-069 and CSM × TRBK-Colombia. Nodal segments of these genotypes, with approximate length of 1 cm, were used. The number of plants formed per explant was evaluated 180 days after inoculation. The experimental design was completely randomized, with ten treatments and 12 repetitions, the data obtained were submitted to analysis of variance (F-test) and the grouped means were analyzed by the Scott-Knott test, in both cases a 5% probability. In vitro culture of explants of the ten genotypes studied presented positive responses to micropropagation in the WPM medium, once the explants of all genotypes showed direct regeneration and formed plants. The citrandarin 'Indio' he stood out with the highest average of explants by plant (4.20).

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Section: Methodsmentioning

confidence: 99%

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Santiago¹,

Santos²,

Lêdo³

et al. 2019

JAS

Self Cite

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“…has been recommended and used as rootstock in substitution of the 'Rangpur' lime, because of its tolerance to the 'citrus tristeza virus', 'citrus decline', 'citrus sudden death', salinity, drought and, additionally, because it provides a good productivity to the grafted cultivars (Siviero et al 2003, Bassanezi et al 2007. Nevertheless, the 'Sunki' mandarin has reduced the number of viable seeds per fruit (two to four), with low polyembryony (< 20 %) and high frequency of zygotic embryos (Weiler et al 2009, Santos et al 2015. Therefore, it is important to study the feasibility of clonal propagation of 'Sunki' mandarin genotypes.…”

Section: Key-wordsmentioning

confidence: 99%

Collection season and auxin treatment in the propagation by cuttings of mandarin hybrids

Sarmiento

Souza

Schwarz

2016

Pesqui. Agropecu. Trop.

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Propagation by cuttings is an alternative for obtaining citrus rootstocks. The exogenous application of indolebutyric acid (IBA), cutting collection season and genotypes used may affect the rooting and vegetative growth of citrus rootstocks cuttings. Two experiments were conducted to study the influence of these factors. In the first one, semi-hardwood cuttings from the 'Sunki' mandarin hybrids H49 and H77 were collected in the fall and late spring of 2013 and treated with IBA (0 mg L-1, 1,500 mg L-1 and 3,000 mg L-1). For each collection season, a 2 x 3 (two genotypes x three IBA concentrations) factorial scheme was adopted, in a randomized blocks design. In the second experiment, the development of cuttings that rooted in the late spring was evaluated until grafting. In this case, a completely randomized experimental design was adopted, with the hybrids H49 and H77. The IBA treatments positively influenced rooting and number of roots only in the fall collection, peaking at 23.3 % of rooted cuttings. In the late spring collection, rooting was close to 100 %, with the IBA treatment being unnecessary. Around 50 % of cuttings from the 'Sunki' mandarin hybrids were ready for grafting at 14 months after cutting.

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“…By defining the optimal sample size, it is possible to reduce costs in both human and financial resources, in addition to the optimization of time (LORENTZ, 2004, SANTOS et al, 2015, it also becomes essential to make good use of the available space, in many occasions it is usually reduced (VALLEJO; MENDOZA, 1992). Another important issue is that sometimes you have valuable plant material that does not have a vast stock so we must optimize the management, since misuse would cause irreparable losses if you do not have copies elsewhere (SANTOS et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Another important issue is that sometimes you have valuable plant material that does not have a vast stock so we must optimize the management, since misuse would cause irreparable losses if you do not have copies elsewhere (SANTOS et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Optimum sample size in quantitative characteristics of seeds of polyembrynic mango

2018

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Determining the sample size in a scientific investigation is important because it gives reliability in the results together with the efficiency in the use of resources and optimization of time. Polyembryony is a characteristic that allows to obtaining clonal and zygotic plants in the same seed. In mango this characteristic is presented, however, there is no information that mentions which is the adequate sample size to evaluate it. The objective of the present work was to determine the optimum sample size by means of the maximum curvature method for future polyembryony studies. Mangoes from the cultivar Manila and Ataulfo were collected from the states of Guerrero and Nayarit, Mexico. In each state, two orchards of each cultivar were chosen, and in each one three trees were selected. In the Fruit Biotechnology laboratory of the Colegio de Postgraduados, Campus Montecillo, five fruit variables were evaluated: weight of fruit, seeds (endocarp and embryos), embryos, weight of the largest embryo and number of embryos. In all the variables evaluated, except for the weight of the largest embryo, the inflection point of the curve was in 6 fruits and an optimal sample size of 8 fruits was determined. The minimum variation coefficients (CV) for fruit weight were from 17.9 to 19.1%, for seed weight 12.7 to 19.3%, embryo weight 12.3 to 17.1% and number of embryos 6.7 to 16.7%. In the case of larger embryo weight, it was determined that 20 fruits are the optimal sample size, obtaining CV from 7 to 22.6%. The fruit and seed weight characteristics were the least variation, and number of embryos with higher CV. The cultivar Manila of Nayarit presented the highest CV in all the variables studied.

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Tamanho Ótimo De Amostras De Frutos E De Sementes Para Determinação Da Poliembrionia Em Citros

Cited by 11 publications

References 11 publications

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Collection season and auxin treatment in the propagation by cuttings of mandarin hybrids

Optimum sample size in quantitative characteristics of seeds of polyembrynic mango

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