The antimicrobial susceptibility, biofilm formation and genotypic profiles of Staphylococcus haemolyticus from bloodstream infections

Silva, Patricia Vollú; Cruz, Raquel Souza; Keim, Luiz Sérgio; Paula, Geraldo Renato de; Carvalho, Bernadete Teixeira Ferreira; Coelho, Leonardo Rocchetto; Carvalho, Maria Cícera da Silva; Rosa, Joatan Machado da; Figueiredo, Agnes Marie Sá; Teixeira, Lenise Arneiro

doi:10.1590/0074-0276108062013022

Cited by 38 publications

(29 citation statements)

References 14 publications

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“…In a study of S. haemolyticus isolated from bloodstream infections, 66% of the isolates formed robust biofilms in vitro , but the same strains were all negative for PCR of the ica operon for PIA synthesis (337). This suggests that S. haemolyticus has other means of biofilm formation.…”

Section: Other Staphylococcal Speciesmentioning

confidence: 99%

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

2016

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The Staphylococci comprise a diverse genus of Gram-positive, non-motile commensal organisms that inhabit the skin and mucous membranes of humans and other mammals. In general, Staphylococci are benign members of the natural flora, but many species have the capacity to be opportunistic pathogens, mainly infecting individuals who have medical device implants or are otherwise immunocompromised. S. aureus and S. epidermidis are a major source of hospital-acquired infections and are the most common causes of surgical site infections and central line-associated bloodstream infections. The ability of Staphylococci to form biofilms in vivo makes them highly resistant to chemotherapeutics and leads to chronic diseases. These biofilm infections include osteomyelitis, endocarditis, medical device implants, and persistence in the cystic fibrosis lung. Here, we provide a comprehensive analysis of our current understanding of Staphylococcal biofilm formation, with an emphasis on adhesins and regulation, while also addressing how Staphylococcal biofilms interact with the immune system. On the whole, this review will provide a thorough picture of biofilm formation of the Staphylococcus genus and how this mode of growth impacts the host.

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Section: Other Staphylococcal Speciesmentioning

confidence: 99%

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

2016

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“…In contrast to the abundance of information available for S. epidermidis, little is known about biofilm formation of S. haemolyticus, despite the importance of this species in nosocomial infections (Barros et al, 2015). These infections are mostly associated with immune-compromised patients and patients with implanted medical devices (Silva et al, 2013). Most S. haemolyticus strains seem to lack the important virulence attributes.…”

Section: Introductionmentioning

confidence: 99%

“…However, some enzymes, cytolysins or surface substances are indicated in the literature as factors contributing to its virulence (Daniel et al, 2014;Flahaut et al, 2008;Simango, 2005), but none of them was identified as a crucial and determinative factor (Fredheim et al, 2009). Despite this, S. haemolyticus is, after S. epidermidis, the second most frequently isolated CNS from clinical cases, particularly from blood infections, including sepsis (Becker et al, 2014;Klingenberg et al, 2007;Liakopoulos et al, 2008;Silva et al, 2013). Therefore, what is the reason for the increasing clinical significance of S. haemolyticus?…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus haemolyticus – an emerging threat in the twilight of the antibiotics age

2015

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“…18 S. epidermidis emerged as a common cause of hospital-acquired infections and S. haemolyticus has been identified as an important cause of primary bloodstream infections associated with the use of central venous catheters. 6,21 Furthermore, they have become multiple drug resistant especially to b-lactams. 14 The increasing antibiotic resistance of nosocomial isolates exacerbates this problem and poses a great challenge for the management of hospital-acquired infections in general.…”

Section: Introductionmentioning

confidence: 99%

Genotypic Diversity of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Inpatients and Outpatients

Talebi

Shafiee

Sadeghi

et al. 2016

Microbial Drug Resistance

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We investigated the prevalence of methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolated from hospitalized patients and outpatients (OP). Out of 350 staphylococcal isolates collected from three hospitals, 190 were coagulase-negative staphylococci (CoNS). These isolates were subjected to antimicrobial susceptibility tests, detection of mecA, and pulsed-field gel electrophoresis (PFGE) typing. Among the 190 isolated CoNS, Staphylococcus epidermidis (47.3%) and Staphylococcus haemolyticus (44.2%) were the most prevalent species. Other CoNS species that were isolated were Staphylococcus saprophyticus (2.1%), Staphylococcus warneri (2.1%), Staphylococcus simulans (1.6%), Staphylococcus capitis (1.1%), Staphylococcus schleiferi (1.1%), and Staphylococcus hominis (0.5%). The rate of resistance to methicillin was 60% with 58 (50%) S. epidermidis and 55 (49%) S. haemolyticus. The rate of resistance to 13 antibiotics tested with the lowest and highest to chloramphenicol and penicillin, respectively. High clonal diversity with different PFGE patterns was obtained for methicillin-resistant S. epidermidis and S. haemolyticus by 32 and 31 types, respectively. Our results indicated that the dissemination of MRCoNS is widespread in Tehran. The majority of these isolates showed distinct genotyping patterns. At the same time, the common patterns were found among the MRCoNS obtained from outpatient and inpatient isolates, suggestive of an epidemiological link.

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The antimicrobial susceptibility, biofilm formation and genotypic profiles of Staphylococcus haemolyticus from bloodstream infections

Cited by 38 publications

References 14 publications

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

Staphylococcus haemolyticus – an emerging threat in the twilight of the antibiotics age

Genotypic Diversity of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Inpatients and Outpatients

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