Trypanosoma janseni n. sp. (Trypanosomatida: Trypanosomatidae) isolated from Didelphis aurita (Mammalia: Didelphidae) in the Atlantic Rainforest of Rio de Janeiro, Brazil: integrative taxonomy and phylogeography within the Trypanosoma cruzi clade

Lopes, Camila Madeira Tavares; Menna-Barreto, Rubem Figueiredo Sadok; Pavan, Márcio G.; Pereira, Mirian Cláudia de Souza; Roque, André Luiz Rodrigues

doi:10.1590/0074-02760170297

Cited by 18 publications

(26 citation statements)

References 30 publications

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“…The barcode sequences of these trypanosomes were submitted to BLAST analysis and aligned with sequences of trypanosomes of the clade T. cruzi available in Genbank, and then phylogenetic inferences were carried out by parsimony analysis. The branching pattern of the inferred dendrogram corroborated with previously known clades within the clade T. cruzi (Lima et al, 2012, 2013; 2015b; Hamilton et al, 2012; Pinto et al, 2012; Cottontail et al, 2014; Botero et al, 2016; Dario et al, 2017a; Espinosa-Álvarez et al, 2018; Lopes et al, 2018) and, in addition, revealed a new clade formed exclusively by sequences from trypanosomes isolated from D. rotundus (Fig. 2).…”

Section: Resultssupporting

confidence: 84%

“…The Neobat lineage also harbors T. wauwau (Lima et al, 2015a), the only species of this lineage obtained in culture and formally described before T. madeirae. The trypanosomes positioned basal to this lineage were T. janseni from a Neotropical marsupial (Lopes et al, 2018), T. noyesii from Australian rodents and marsupials (Hamilton et al, 2012b; Botero et al, 2016), and one unnamed trypanosome of lemurs from Madagascar known just by a small DNA sequence (Larsen et al, 2016) (Fig. 2, Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The diversity of trypanosome species infecting bats in the New and Old Words nested mostly in the clade T. cruzi (Hamilton et al, 2012; Pinto et al, 2012; Cottontail et al, 2014; Lima et al, 2012, 2013, 2015a,b; Barbosa et al, 2016; Espinosa-Álvarez et al, 2018); T. evansi and T. vegrandis are the only trypanosome species found in bats (and other mammals) that could not be nested into this clade (Ramírez et al, 2014; Austen et al, 2015). The clade T. cruzi also comprises trypanosomes from Australian (Hamilton et al, 2012; Botero et al, 2016) and Brazilian (Lopes et al, 2018) marsupials.…”

Section: Introductionmentioning

confidence: 99%

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Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Barros

Lima

Schubach

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Molecular phylogenetic studies have revealed the growing diversity of bat trypanosomes. Here, 14 isolates from blood samples of the vampire bat Desmodus rotundus (Phyllostomidae) from Rio de Janeiro, Southeast Brazil, were cultivated, and morphologically and molecularly characterized. All isolates represent a novel species named Trypanosoma madeirae n. sp. positioned in the Neobat lineage of the clade T. cruzi. The Neobat lineage also comprises closely related trypanosomes of clades Neotropic 1, 2 and 3 from diverse phyllostomid species. Trypanosomes of Neotropic 1, found in Trachops cirrhosus and Artibeus jamaicensis (phyllostomids), likely represent a different species or genotype closely related to T. madeirae. Consistent with its phylogenetic positioning, T. madeirae differs from Trypanosoma cruzi in morphology of both epimastigote and trypomastigote culture forms and does not infect Triatoma infestans. Similar to its closest relatives of Neobat lineage, T. madeirae was unable to develop within mammalian cells. To date, PCR-surveys on archived blood/liver samples unveiled T. madeirae exclusively in D. rotundus from Southern to Northern Brazil. The description of a new species of bat trypanosome associated with vampire bats increases the repertoire of trypanosomes infecting D. rotundus, currently comprised of Trypanosoma cruzi, T. cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli, Trypanosoma pessoai, and Trypanosoma madeirae.

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Section: Resultssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Barros

Lima

Schubach

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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“…T. cruzi is primarily an enzooty of wild mammals, infects hundreds of mammalian species and is transmitted by dozens of triatomine species throughout all of the biomes of America between the southern United States and South Argentina (Brenière et al, 2016; Jansen et al, 2018). Marsupials and bats are recognized as very ancient hosts of T. cruzi clade (Stevens et al, 1998; Hamilton et al, 2012; Lopes et al, 2018). However, the origin of this clade still remains inconclusive.…”

Section: Introductionmentioning

confidence: 99%

Uncovering Trypanosoma spp. diversity of wild mammals by the use of DNA from blood clots

Rodrigues

Lima

Xavier

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Trypanosoma spp. infection in wild mammals is detected mainly through parasitological tests that usually display low sensitivity. We propose the use of DNA extracted directly from blood clots (BC), which are neglected sources of DNA for diagnosis and identification of Trypanosoma spp. This approach followed by nested PCR targeting the 18S SSU rDNA demonstrated to be sensitive and suitable to evaluate the diversity of trypanosomes infecting sylvatic mammals, including subpatent and mixed infections. Infection was detected in 95/120 (79.2%) samples from bats, carnivores and marsupials that included negative serological and hemoculture testing mammals. Thirteen Trypanosoma spp. or Molecular Operational Taxonomic Units (MOTUs) were identified, including two new MOTUs. The high diversity of trypanosomes species and MOTUs infecting bats and marsupials showed that these hosts can be considered as bio-accumulators of Trypanosoma spp., with specimens of Didelphis spp. displaying the highest trypanosome diversity. The use of blood clots allowed direct access to non-culturable parasites, mixed infections, besides bypassing the selective pressure on the parasites inherent to cultivation procedures. Trypanosoma cruzi was the species found infecting the highest number of individuals, followed by T. lainsoni. Positive PCR for T. cruzi was observed in 16 seronegative individuals and 30 individuals with negative hemocultures. Also, T. lainsoni , previously found only in rodents, showed to be capable of infecting bats and marsupials. This finding makes it clear that some species of Trypanosoma are more generalist than previously thought. Molecular diagnosis using nested PCR from DNA extracted from BC allowed the increase of the knowledge about host-spectrum and distribution of Trypanosoma spp. and allowed the identification of new MOTUs.

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“…Although small mammals from this biome have been reported as reservoirs of several microorganisms including Trypanosoma sp. in Didelphis aurita (Lopes, Menna‐Barreto, Pavan, Pereira, & Roque, ) and in Trinomys sp. (Trüeb et al., ); Hantavirus (Juquitiba viral genotype) in Akodon cursor and Oligoryzomys nigripes (De Oliveira et al., ); and Toxoplasma gondii in Akodon spp., O. nigripes and D .…”

Section: Introductionmentioning

confidence: 99%

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

Vieira

D’Andrea

Vilela

et al. 2019

Transbound Emerg Dis

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Leptospirosis is a common worldwide bacterial zoonosis and has been studied in One Health approaches. Small mammals are described as the most important maintenance reservoirs of several pathogens in nature, including leptospires. The aim of this study was to identify infection by leptospires among small mammals on the Atlantic forest biome and evaluate their potential as carriers of these spirochetes. A total of 153 small mammals belonging to orders Rodentia and Didelphimorphia (distributed on 17 genera and 22 species) were captured. Blood and kidney samples were collected from animals and a conventional PCR targeted on lipL32 gene was conducted on renal tissues. Species identification was performed in eight samples by sequencing of rrs gene. A total of 28% of the animals presented lipL32 PCR‐positive, and four pathogenic Leptospira species (L. interrogans, L. borgpetersenii, L. santarosai and L. noguchii) were identified. This study highlights the role of small mammals as carriers of leptospires on the Atlantic Forest representing a potential source of pathogenic Leptospira spp infection for both humans and domestic animals.

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Trypanosoma janseni n. sp. (Trypanosomatida: Trypanosomatidae) isolated from Didelphis aurita (Mammalia: Didelphidae) in the Atlantic Rainforest of Rio de Janeiro, Brazil: integrative taxonomy and phylogeography within the Trypanosoma cruzi clade

Cited by 18 publications

References 30 publications

Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Uncovering Trypanosoma spp. diversity of wild mammals by the use of DNA from blood clots

PathogenicLeptospiraspecies are widely disseminated among small mammals in Atlantic Forest biome

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