Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals

Kremer, Frederico Schmitt; Eslabão, Marcus Redü; Jorge, Sérgio; Oliveira, Natasha Rodrigues de; Labonde, Julia; Santos, Monize N. P; Monte, Leonardo Garcia; Grassmann, André Alex; Cunha, C. E.; Forster, Karine M.; Moreno, Luisa Zanolli; Moreno, Andréa Micke; Campos, Vinícius Farias; McBride, Alan J. A.; Pinto, Luciano S.; Dellagostin, Odir A.

doi:10.1590/0074-02760160010

Cited by 16 publications

(5 citation statements)

References 36 publications

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“…During the final assembly, the genome of L. interrogans strain Piscina was aligned to the genome of L. interrogans strain Fiocruz L1-130 using Artemis Comparison Tool (ACT) ( Rutherford et al 2000 ) to identify structural variations (e.g., translocations, large insertions/deletions, inversions). To understand the plasticity of the L. interrogans genome, it was also compared to three other strains of L. interrogans - RCA, Prea, and Capivara - from the same sequence type (ST:17), which were reported by our group in a previous study ( Kremer et al 2016b ). These strains were obtained from different mammal maintenance hosts in Pelotas, southern Brazil.…”

Section: Methodsmentioning

confidence: 99%

“…Phylogenetic analysis - Mugsy ( Angiuoli & Salzberg 2011 ) was used to identify syntenic regions shared between the genome of strain Piscina; draft genomes of the L. interrogans strains RCA, Prea, Capivara, and Aceguá that we have previously described ( Kremer et al 2016b ); and the finished genomes of L. interrogans (nearest phylogenetic relatives) (Supplementary data, ) already available at GenBank. The regions present in all genomes were selected and merged into a single alignment, which was processed by the tools “TreeConstruction.Distance-Calculator” and “TreeConstruction.DistanceTreeConstructor” from the Phylo module from the Biopython package ( http://biopython.org /) to generate a neighbour-joining tree, which was plotted using the interactive tree of life server ( http://itol.embl.de /).…”

Section: Methodsmentioning

confidence: 99%

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Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Jorge

Kremer

Oliveira

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUNDLeptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates.OBJECTIVESThis study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features.METHODSThe whole genome was sequenced by next-generation sequencing (Ion Torrent). The genome was assembled, scaffolded, annotated, and manually reviewed. Mutations were identified based on a variant calling analysis using the genome of L. interrogans strain Fiocruz L1-130 as a reference.FINDINGSThe entire genome had an average GC content of 35%. The variant calling analysis identified 119 single nucleotide polymorphisms (SNPs), from which 30 led to a missense mutation. The structural analyses identified potential evidence of genomic inversions, translocations, and deletions in both the chromosomes.MAIN CONCLUSIONSThe genome properties provide comprehensive information about the local isolates of Leptospira spp., and thereby, could facilitate the identification of new targets for the development of diagnostic kits and vaccines.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Jorge

Kremer

Oliveira

et al. 2018

Mem. Inst. Oswaldo Cruz

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“…Genomic analyses of pathogens and enhanced understanding of the mechanisms of pathogenesis have resulted in new antigen discovery and the development of recombinant veterinary vaccines. A large amount of draft and whole-genome sequencing of viruses, prokaryotes and eukaryotes pathogens have been performed (Pizza et al 2000;Tettelin et al 2000;Vasconcelos et al 2005;Kremer et al 2016). These advancements have also improved antigen discovery and the characterization of variability between viral pathogens, which typically contain fewer than ten genes, and eukaryotic pathogens, which often encode >10 000 genes (Cho et al 2002;Aurrecoechea et al 2007).…”

Section: Biotechnology Applied To Next Generation Vaccine Developmentmentioning

confidence: 99%

Vaccination and Immunology in Large Animals

2022

Int J Vet Sci

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“…In 2011, an international collaboration, the Leptospira Genome Project, was established with the objective to sequence the genomes of all known Leptospira spp. In addition, several individual research groups have contributed by sequencing the genomes of newly-isolated strains [ 15 , 30 , 73 , 74 ]. To date, hundreds of leptospiral genome sequences are available in public databases, e.g., more than 280 L. interrogans genome sequences are accessible.…”

Section: Leptospiral Genomes As a Source Of Vaccine-related Informmentioning

confidence: 99%

Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates

Dellagostin

Grassmann

Rizzi

et al. 2017

IJMS

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Leptospirosis is a major public health problem with an incidence of over one million human cases each year. It is a globally distributed, zoonotic disease and is associated with significant economic losses in farm animals. Leptospirosis is caused by pathogenic Leptospira spp. that can infect a wide range of domestic and wild animals. Given the inability to control the cycle of transmission among animals and humans, there is an urgent demand for a new vaccine. Inactivated whole-cell vaccines (bacterins) are routinely used in livestock and domestic animals, however, protection is serovar-restricted and short-term only. To overcome these limitations, efforts have focused on the development of recombinant vaccines, with partial success. Reverse vaccinology (RV) has been successfully applied to many infectious diseases. A growing number of leptospiral genome sequences are now available in public databases, providing an opportunity to search for prospective vaccine antigens using RV. Several promising leptospiral antigens were identified using this approach, although only a few have been characterized and evaluated in animal models. In this review, we summarize the use of RV for leptospirosis and discuss the need for potential improvements for the successful development of a new vaccine towards reducing the burden of human and animal leptospirosis.

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Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals

Abstract: In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively.

Cited by 16 publications

References 36 publications

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Vaccination and Immunology in Large Animals

Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates

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