Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region

Ribeiro, Edlainny Araujo; Gales, Ana Cristina; Oliveira, Ana Paula Streling de; Coelho, Danilo Dias; Oliveira, Rodrigo Alves de; Pfrimer, Irmtraut Araci Hoffmann; Filho, José Rodrigues do Carmo

doi:10.1590/0037-8682-0087-2020

Cited by 6 publications

(4 citation statements)

References 9 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In keeping with this, this pathogen's role in seafood samples is potentially unknown, and further research is required to understand this fact. High prevalence of resistance of A. baumannii strains toward commonly-used antimicrobials, particularly tetracycline, gentamicin, ciprofloxacin, trimethoprim, streptomycin, and erythromycin was reported from Brazil [41], Iran [42], United States [43], Jordan [44], Africa [45], and Korea [46].As antibiotic-resistant A. baumannii was detected in large number of seafood samples. Proper monitoring of the seafood microbial quality should perform at the site of fishing and also after their transport.…”

Section: Discussionmentioning

confidence: 99%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

View full text Add to dashboard Cite

A cinetobacterbaumannii is a newly-launched bacterium responsible for rare cases of diarrhea and foodborne diseases. This survey was performed to study the prevalence and antimicrobial resistance of A. baumannii isolates recovered from fish, shrimp, and lobster. Five-hundred seafood samples were collected and cultured for isolation of A. baumannii. A. baumannii isolates were identified by biochemical tests and further confirmed by the PCR. The antimicrobial resistance of isolates was studied using disk diffusion test. Twenty-eight out of 500 (5.6%) seafood samples were contaminated with A. baumannii. Fish had the highest prevalence of A. baumannii (10%), while lobster had the lowest (2.5%). A. baumannii isolates harbored the highest incidence of resistance toward tetracycline (75%), gentamicin (71.4%), ciprofloxacin (64.2%), trimethoprim (57.1%), streptomycin (53.5%), and erythromycin (50%) antimicrobial agents. The lowest incidence of resistance was obtained against chloramphenicol (3.5%) and imipenem (7.1%) antimicrobial agents. This is the first report of isolation and identification and antimicrobial resistance pattern of A. baumannii in seafood samples globally. The role of seafood samples as reservoirs of antimicrobial-resistant A. baumannii was determined. Complete cooking of seafood and prevention from their consumption in raw form can diminish the risk of A. baumannii foodborne diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The emergence of resistant strains, as well as tolerance to disinfectants used for cleaning in hospital environments, predictably pose increased risks to human health (Chin et al 2018). The spread of A. baumannii with multi-drug-resistant antimicrobial profiles has been reported to be rapid (Ribeiro et al 2020), and this bacterium contributes to increased morbidity and mortality in patients (Espinal et al 2011;Hou and Yang 2015), which accordingly warrants effective surveillance and antimicrobial treatment.…”

Section: Molecular Analysis Of the Prevalence Of Acinetobacter Bauman...mentioning

confidence: 99%

Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study

Silva

Miguel²,

Júnior³

et al. 2023

Biosci. J.

View full text Add to dashboard Cite

Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.

show abstract

“…18 Além disso, no hospital em estudo, já foi possível evidenciar que 55,6% dos A. baumannii isolados carreavam o gene blaOXA-23, associado à resistência aos carbapenêmicos, uma das últimas opções terapêuticas em infecções graves. 19 Portanto, o perfil fenotípico de multirresistência descrito nesta pesquisa sugere a necessidade de medidas mais severas para controle de infecção, uso racional de antimicrobianos e vigilância contínua. 19 Apesar das recomendações robustas acerca das boas práticas assistenciais, uma avaliação em unidades ambulatoriais de hemodiálise, constatou que a indicação e duração para a profilaxia não são consistentes com as diretrizes em 20% dos casos, o que pode piorar o panorama associado a resistência bacteriana e o prognóstico dos pacientes.…”

Section: Dessaunclassified

“…19 Portanto, o perfil fenotípico de multirresistência descrito nesta pesquisa sugere a necessidade de medidas mais severas para controle de infecção, uso racional de antimicrobianos e vigilância contínua. 19 Apesar das recomendações robustas acerca das boas práticas assistenciais, uma avaliação em unidades ambulatoriais de hemodiálise, constatou que a indicação e duração para a profilaxia não são consistentes com as diretrizes em 20% dos casos, o que pode piorar o panorama associado a resistência bacteriana e o prognóstico dos pacientes. [20][21] Além disso, é preciso considerar que as instalações de diálise ambulatorial são ambientes propícios para a disseminação de organismos multirresistentes, reforçando a necessidade da identificação e investigação de cepas semelhantes entre os pacientes e o ambiente, evidenciando a ocorrência de transmissão cruzada.…”

Section: Dessaunclassified

Occurrence and resistance profile of bacteria belonging to the ESKAPE group in hemodialysis patients

Ribeiro

Alves²,

Alves³

2023

Rev. Pre Infec e Saúde

View full text Add to dashboard Cite

Introduction: Hemodialysis is the main treatment for patients with Chronic Renal Insufficiency and one of the main complications resulting from it is the occurrence of infections caused by opportunistic microorganisms belonging to the “ESKAPE” group. Aim: to determine the frequency of isolation of pathogens from the ESKAPE group and evaluate their respective antimicrobial resistance profiles in hemodialysis patients in a medium and high complexity hospital located in the Amazon. Outlining: Retrospective study carried out with collection of data available in the medical records of 167 patients diagnosed with Chronic Renal Insufficiency, undergoing hemodialysis or peritoneal dialysis from 2015 to 2019 in a regional hospital in the Southeast of Pará. Results: Of the 174 bacteriological cultures analyzed, 70.1% were positive and, of these, 57.4% were positive for bacteria belonging to the ESKAPE group. The most frequent microorganism belonging to the ESKAPE group was Staphylococcus aureus (47.1%); as to the resistance levels, 50% of Acinetobacter baumannii were resistant to carbapenems. Implications: The phenotypic profile of multidrug resistance to antimicrobials in strains of Acinetobacter baumannii was identified, highlighting the importance of knowledge about the profile of sensitivity to antimicrobials for a more assertive treatment and better prognosis.

show abstract

Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region

Cited by 6 publications

References 9 publications

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study

Occurrence and resistance profile of bacteria belonging to the ESKAPE group in hemodialysis patients

Contact Info

Product

Resources

About