Localization and distribution of CCK-8, NPY, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)

Barrios, Carlos; Santinón, Juan José; Domitrovic, Hugo Alberto; Sánchez, Sebastian; Hernández, David Roque

doi:10.1590/0001-3765202020181165

Cited by 10 publications

(6 citation statements)

References 70 publications

(51 reference statements)

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“…Furthermore, due to a re-sidual gastric acidification and pepsin activity, the stomach plays a negligible role in protein hydrolysis of Senegalese sole and the intestinal proteolysis phase is of primordial importance in the digestion process (Yúfera and Darias, 2007;Gilannejad et al, 2017). Therefore, the CCK-8 distribution pattern in our study is closely related to those generally found in fish with omnivorous feeding habits (Barrios et al, 2020). This characteristic distribution of CCK-producing cells has been associated with CCK specific roles in regulating the gut motility in different GIT segments and local signalling functions, and may underlie the relatively fast transit of undigested food, due to the lack of the controlling role of a functional stomach acting as a short term reservoir (Hartviksen et al, 2009;Gra¨ns and Olsson, 2011;Lie et al, 2018;Le et al, 2019).…”

Section: Git Cck Distributionsupporting

confidence: 68%

Daily rhythms of intestinal cholecystokinin and pancreatic proteases activity in Senegalese sole juveniles with diurnal and nocturnal feeding

Gilannejad

Rønnestad

Lai

et al. 2021

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Git Cck Distributionsupporting

confidence: 68%

Daily rhythms of intestinal cholecystokinin and pancreatic proteases activity in Senegalese sole juveniles with diurnal and nocturnal feeding

Gilannejad

Rønnestad

Lai

et al. 2021

Comparative Biochemistry and Physiology Part A: Molecular &

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“…To our knowledge, this is the first report to describe Npy-ir cells in a Neotropical cichlid species. The pattern observed in C. dimerus was also described for several species, as O. niloticus (Pereira et al, 2017), Rhamdia quelen (Hernández et al, 2018), Odontesthes bonariensis (Vigliano et al, 2011), Chanos chanos (Lin et al, 2017), Salminus brasiliensis (Pereira et al, 2015), Ictalurus punctatus (Min et al, 2009), Prochilodus lineatus (Barrios et al, 2020), Colossoma macropomum, Pseudoplatystoma reticulatum × Leiarius marmoratus (Pereira et al, 2020). The anterior portion of the intestine would be a source of peripheral signals capable of stimulating food intake when the gut is empty (Vigliano et al, 2011).…”

Section: Discussionmentioning

confidence: 64%

“…3I), decreasing in density from anterior to posterior (Table 1). These cells were open-type (Barrios et al, 2020), with a narrow and elongated shape and a round nucleus. The cytoplasm was projected to the apical and basal regions, with strong immunolabel in the perinuclear zone.…”

Section: Npy-ir Cells Localizationmentioning

confidence: 99%

Anatomy and histology of the digestive tract and immunolocalization of Npy (orexigenic peptide) in the fish Cichlasoma dimerus (Cichliformes: Cichlidae)

et al. 2022

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Introduction: The digestive tract of Neotropical cichlids has not been deeply studied, and it is a fundamental topic for understanding fish physiology, nutrition, trophic associations, and evolution. Objective: To describe anatomically and histologically the digestive tract of the Neotropical cichlid fish Cichlasoma dimerus and to immunolocalize the orexigenic peptide (Npy) along the intestine. Methods: We euthanized 14 adult individuals and fixed the organs in Bouin´s solution; we stained 7 μm thick paraffin sections for general description and with Alcian Blue (pH = 2.5, AB) and Periodic acid-Schiff (PAS) to identify acid or neutral glycoconjugates, respectively. Additionally, 3 adult individuals to immunohistochemistry for Npy, we manually counted PAS- and AB-positive cells, and Npy-immunoreactive cells per fold. Results: There is a short oesophagus, a sac-like stomach, and a tubular intestine with two loops. The oesophagus has a stratified epithelium with a high density of PAS- and AB-positive goblet cells and striated muscle fibes in the tunica muscularis. The stomach mucosa is formed by simple columnar epithelium. The intestine has a simple columnar epithelium, with brush border and interspersed PAS- and AB-positive goblet cells, and Npy-immunoreactive cells. There is an ileorectal valve in the transition between the posterior intestine and the rectum. This last gut portion has goblet cells and a thicker tunica muscularis. Conclusions: C. dimerus shares features with other Neotropical cichlids, but the goblet cells and gastric glands distribution seems to be unique for the species. To our understanding, this is the first work to describe Npy-immunoreactive cells distribution in the intestine of a Neotropical cichlid fish.

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“…The cell viability was detected by CCK-8 assay accroding to the study reported by Barrios et al [ 18 ]. Briefly, cells were placed in 96‐well plates, treated with IR, and incubated with 10 μl of CCK‐8 reagent (Beyotime).…”

Section: Methodsmentioning

confidence: 99%

Knockdown of DNA polymerase ζ relieved the chemoresistance of glioma via inhibiting the PI3K/AKT signaling pathway

et al. 2021

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Previous reports suggest that DNA polymerase ζ is highly expressed in glioma tissues. The present study aimed to investigate the roles of the REV7 subunit of DNA polymerase ζ in glioma cell chemoresistance and its underlying mechanisms. The bioinformatics method was used to compare the expression of REV7 in glioma and normal tissues. The expression of REV7 in glioma tumor samples and the adjacent tissue was examined by reverse transcription polymerase chain reaction. Moreover, an in vitro analysis using glioma cells was used to test the effects of REV7 siRNA on the proliferation and apoptosis of glioma cell line U251 cells, and the effect of REV7 siRNA on the sensitivity of the U251 cells to cisplatin was also explored. The expression of REV7 in glioma tumors was significantly increased. Moreover, the knockdown of REV7 in glioma cells decreased the proliferation and increased the apoptosis of U251 cells; moreover, REV7 siRNA also increased the sensitivity of U251 cells to cisplatin. Finally, REV7 may regulate the proliferation, apoptosis, and chemosensitivity of U251 cells by affecting phosphoinositide 3-kinase signaling. Our data suggest that REV7 is involved in the chemosensitivity of glioma cells and provides a theoretical basis for targeting DNA polymerase ζ to improve the sensitivity of glioma cells to chemotherapy.

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Localization and distribution of CCK-8, NPY, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)

Cited by 10 publications

References 70 publications

Daily rhythms of intestinal cholecystokinin and pancreatic proteases activity in Senegalese sole juveniles with diurnal and nocturnal feeding

Daily rhythms of intestinal cholecystokinin and pancreatic proteases activity in Senegalese sole juveniles with diurnal and nocturnal feeding

Anatomy and histology of the digestive tract and immunolocalization of Npy (orexigenic peptide) in the fish Cichlasoma dimerus (Cichliformes: Cichlidae)

Knockdown of DNA polymerase ζ relieved the chemoresistance of glioma via inhibiting the PI3K/AKT signaling pathway

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