Long noncoding RNA (lncRNA) DUXAP10 has been shown to act as an oncogene in various tumors; however, its roles in glioma progression have never been established. Here, we show that DUXAP10 is overexpressed in glioma tissues and cells. Loss of function experiments reveal that DUXAP10 knockdown has little effects on glioma cell viability, but significantly reduces the stemness of glioma cells, which is characterized as the decrease of stemness marker expression, tumor sphere‐forming ability, and ALDH activity. RNA immunoprecipitation and immunofluorescence assays indicate that DUXAP10 can directly interact with HuR protein and suppress the cytoplasm‐nuclear translocation of HuR, which subsequently enhances Sox12 mRNA stability in cytoplasm and thus increases Sox12 expression. Further rescuing experiments show that the HuR/Sox12 axis is responsible for DUXAP10‐mediated effects on glioma cell stemness.
Excessive iron ions in cancer cells can catalyze H 2 O 2 into highly toxic •OH and then promote the generation of reactive oxygen species (ROS), inducing cancer ferroptosis. However, the e cacy of ferroptosis catalyst is still insu cient because of low Fe(II) release, which severely limited its application in clinics. Herein, we developed a novel magnetic nanocatalyst for MRI-guided chemo-and ferroptosis synergistic cancer therapies through iRGD-PEG-ss-PEG modi ed gadolinium engineering magnetic iron oxide loaded Dox (ipGdIO-Dox). The introduction of gadolinium compound disturbed the structure of ipGdIO-Dox, making magnetic nanocatalyst be more sensitive to weak acid. When the ipGdIO-Dox entered into cancer cells, abundance of Fe(II) ions were released and then catalyzed H 2 O 2 into highly toxic OH•, which would elevate cellular oxidative-stress to damage mitochondria and cell membranes and induced cancer ferroptosis. In addition, the iRGD-PEG-ss-PEG chain coated onto nanoplatform were also broken by high expression of GSH, and then the Dox was released. This process not only effectively inhibited DNA replication, but further activated cellular ROS, making nanoplatform achieve stronger anticancer ability. Besides, the systemic delivery ipGdIO-Dox signi cantly enhanced T 1 -and T 2 -weighted MRI signal of tumor, endowing accurate diagnostic capability for tumor recognition. Therefore, the ipGdIO-Dox might be a promising candidate for developing MRI guided chemo-and chemdynamic synergistic theranostic system.
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