Subclinical Diabetes

Lima, L.M.T.R.

doi:10.1590/0001-3765201720160394

Cited by 23 publications

(14 citation statements)

References 159 publications

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“…We reasoned whether the typical lack of signal for oligomers in rodents could be an issue of tinctorial responsiveness or a dissimilar antibody titer between human and murine amylin aggregates, a feature also observed for other amylin variants [10]. In fact, such as OLIM could be detected in pancreas of elder humans without diagnosed overt diabetes [21], although a hyperhormonemic subclinical diabetes condition [22,23] could be responsible for such outcome. We were able to detect OLIM responsiveness for the A11 in 16-week old mice (Fig.…”

Section: Morphological and Immunochemical Properties Of Human And Murine Amylin Aggregates -Inmentioning

confidence: 99%

The toxic nature of murine amylin and the immune responsivity of pancreatic islet to conformational antibody in mice

et al. 2018

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The human amylin is a pancreatic peptide hormone found in hyperhormonemic state along with insulin in subclinical diabetes. Amylin has been associated with the pathology of type 2 diabetes, particularly due to its ability to assembly into toxic oligomers and amyloid specimens. On the other hand, some variants such as murine amylin has been described as non-amyloidogenic, either in vitro or in vivo. Recent data have demonstrated the amyloid propensity of murine amylin and the therapeutic analogue pramlintide, suggesting a universality for amylin amyloidosis. Here, we report the amyloidogenesis of murine amylin, which showed lower responsivity to the fluorescent probe thioflavin T compared to human amylin, but presented highly organized fibrilar amyloid material. The aggregation of murine amylin also resulted in the formation of cytotoxic specimens, as evaluated in vitro in INS-1 cells. The aggregation product from murine amylin was responsive to a specific antibody raised against amyloid oligomers, the A11 oligomer antibody. Pancreatic islets of wild-type Swiss male mice have also shown responsivity for the anti-oligomer, indicating the natural abundance of such specimen in rodents. These data provide for the first time evidences for the toxic nature of oligomeric assemblies of murine amylin and its existence in wild-type, non-transgenic mice.

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Section: Morphological and Immunochemical Properties Of Human And Murine Amylin Aggregates -Inmentioning

confidence: 99%

The toxic nature of murine amylin and the immune responsivity of pancreatic islet to conformational antibody in mice

et al. 2018

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“…The semiarid region of the Brazilian Northeast is known for hosting a number of native species that are under systematic exploitation in recent years, without any sustainable management, which ultimately causes the extinction of some species, thus unleashing a rural exodus. Thus, the therapeutic potential of native plants must be scientifically demonstrated, so that it can be exploited in a rational and orderly manner [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Screening of Bioactivities and Toxicity ofCnidoscolus quercifoliusPohl

Paredes

Vasconcelos

Paim

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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The caatinga, an exclusively Brazilian biome, is one of the most endangered vegetation systems in the planet. To be exploited rationally, its potential needs to be scientifically demonstrated. Among these is the faveleira, used in northeastern Brazil. It stands out for its extraordinary drought resistance and medicinal properties. The objective of this study was to assess the therapeutic potential of compounds extracted from Cnidoscolus quercifolius Pohl in preventing disease and its rational use as a herbal therapeutic tool. The methodology began with the collection and herborization of the plant material, to obtain the chemical compounds, preliminary phytochemical analysis, and extraction of the constituents of the active extracts. To determine the biological activities the authors conducted investigation of antioxidant and antimicrobial activities, inhibition capacity of the acetylcholinesterase enzyme, and initial assessment of toxicity of the extracts. The results demonstrated great potential as an antimicrobial agent, an important antioxidant capacity, and acetylcholinesterase inhibition response with no significant difference compared with the reference drug. The authors expect to develop a new herbal product, resulting in lower production costs and that, consequently, could be commercialized in more accessible form to the population, highlighting the risk reduction of contraindication of this category of medications.

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“…3 ). Such condition may resemble a subclinical diabetes condition [62,63], characterized by normoglycemic hyperinsulinemia.…”

Section: Discussionmentioning

confidence: 99%

Zinc restriction promotes β-cell hyper-hormonemia and endocrine pancreas degeneration in mice

Sisnande

Lima

Silva

et al. 2019

Preprint

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Zinc is a key component of proteins, including interaction with varying pancreatic hormones, including insulin and amylin. Zinc is key in insulin crystallinity in ZnT8 knock-out mice models, although the dietary role of zinc restriction over both energetic metabolism and ß-pancreatic hormonemia and morphology remained unexplored. We aimed to test whether dietary zinc restriction on swiss male mice would impact over endocrine pancreas and metabolic phenotype. We evaluated the role of dietary zinc restriction on ß-pancreatic hormonemia on non-transgenic Swiss male mice weaned onto a control or low-zinc diet for 4 weeks. Growth, glycemia, insulinemia, amylinemia and pancreatic islet were smaller in intervention group despite insulin crystallinity in secretory granules. We have found overlabelling for insulin, amylin and toxic oligomers in apoptotic pancreatic islet. High production of β-pancreatic hormones in zinc-restricted animals counteract the decreasing islet size due to their apoptotic cells. We conclude that zinc deficiency is sufficient to promote islet β-cell hormonal disruption and degeneration.

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Subclinical Diabetes

Cited by 23 publications

References 159 publications

The toxic nature of murine amylin and the immune responsivity of pancreatic islet to conformational antibody in mice

The toxic nature of murine amylin and the immune responsivity of pancreatic islet to conformational antibody in mice

Screening of Bioactivities and Toxicity ofCnidoscolus quercifoliusPohl

Zinc restriction promotes β-cell hyper-hormonemia and endocrine pancreas degeneration in mice

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