Production and Enhancement of Omega-3 Fatty Acid from<i>Mortierella alpina</i>CFR-GV15: Its Food and Therapeutic Application

Vadivelan, Ganesan; Venkateswaran, G.

doi:10.1155/2014/657414

Cited by 45 publications

(19 citation statements)

References 27 publications

(38 reference statements)

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“…According to Vadivelan and Venkateswaran (2014), the physiology of fungi belonging to the Mortierella alpina species can be altered by mutation induction, chemical inhibitors, and stress conditions, among other factors, in order to increase omega-3 fatty acids (EPA and DHA) production.…”

Section: Fatty Acids Profile In Fungal Biomassmentioning

confidence: 99%

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Fungi as an alternative to produce essential fatty acids

Francisco¹,

Saldanha²,

Fraga³

2017

Científica

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Fungi have the ability to produce essential fatty acids of the omega-3 family, what is of great scientific interest, since these microorganisms are capable of developing under varied conditions, making their production a viable alternative. Fatty acids are of great importance in human food due to their beneficial effects to the organism, such as reduction in the development of cardiovascular problems and other chronic-degenerative diseases. Currently, the main food source of omega-3 fatty acids is saltwater fish, but there are concerns about potential health risks, including environmental contaminants, such as some metals, as well as ecological issues related to the extinction of some species. Therefore, interest in the biotechnology area has been increasing with the aim of extracting bioactive compounds from certain fungi species, besides using these species to produce biomass, food, and energy, among others.Additional keywords: biomass; biotechnology; industrial application; polyunsaturated fatty acids. ResumoA capacidade de produção de ácidos graxos essenciais da família ômega 3 por fungos é de grande interesse científico, uma vez que estes microrganismos são capazes de se desenvolver em condições variadas, apresentando-se como alternativa viável para a produção dos mesmos. Os referidos ácidos graxos são de grande importância na alimentação humana, devido seus efeitos benéficos ao organismo, como, por exemplo, a redução no desenvolvimento de problemas cardiovasculares e de outras doenças crônico-degenerativas. Atualmente, a principal fonte alimentar de ácidos graxos ômega 3 é a ingestão de pescados marinhos, mas existem algumas preocupações sobre os riscos potenciais para a saúde, incluindo contaminantes ambientais, tais como alguns metais, além de questões ecológicas relacionadas com a extinção de algumas espécies. Portanto, o interesse pela área da biotecnologia vem crescendo com o objetivo de extrair compostos bioativos de certas espécies de fungos, além de sua utilização para produção de biomassas, alimentos, energia, dentre outros de importância econômica.Palavras-chave adicionais: ácidos graxos poli-insaturados; aplicação industrial; biomassa; biotecnologia.

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Section: Fatty Acids Profile In Fungal Biomassmentioning

confidence: 99%

“…Vadivelan & Venkateswaran (2014) believe that processes using microorganisms to produce polyunsaturated fatty acids are reliable and economically attractive by producing beneficial compounds. In addition, these processes can be efficient for large-scale production for food or therapeutic purposes.…”

Section: Fungal Biomass Products Applicationmentioning

confidence: 99%

Fungi as an alternative to produce essential fatty acids

Francisco¹,

Saldanha²,

Fraga³

2017

Científica

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“…GC analysis of total lipid revealed the presence of EPA (3.40 ± 0.1%), DHA (4.32 ± 0.1%), and n-6 PUFAs AA (56.7 ± 0.2%) [19]. A significantly higher accumulation of AA was achieved within seven days of fermentation.…”

Section: Resultsmentioning

confidence: 99%

“…Genomic DNA was extracted and the nucleotide sequence was analyzed by BLAST and deposited in NCBI [19]. Further the omega-3 desaturase gene was subjected to polymerase chain reaction.…”

Section: Methodsmentioning

confidence: 99%

Influence of Supplementation of Vegetable Oil Blends on Omega-3 Fatty Acid Production inMortierella alpinaCFR-GV15

Vadivelan

Rao

Venkateswaran

2017

BioMed Research International

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Objectives of this study were designed for improved production of mycelial omega-3 fatty acids with particular reference to EPA and DHA from the oleaginous fungus Mortierella alpina CFR-GV15 under submerged low temperatures fermentation supplemented with linseed oil and garden cress oil as an additional energy source. The fungus was grown at 20°C temperature for four days initially followed by 12°C temperature for next five days. The basal medium contained starch, yeast extract, and a blend of linseed oil (LSO) and garden cress oil (GCO) in the ratio 1 : 1. Results of the study revealed that, after nine days of total incubation period, the enhancement of biomass was up to 16.7 g/L dry weight with a total lipid content of 55.4% (v/w). Enrichment of omega-3 fatty acids indicated a significant increase in fatty acid bioconversion (ALA 32.2 ± 0.42%, EPA 7.9 ± 0.1%, and DHA 4.09 ± 0.2%) by 2.5-fold. The two-stage temperature cultivation alters the fatty acid profile due to activation of the desaturase enzyme in the cellular levels due to which arachidonic acid (AA) content reduced significantly. It can be concluded that Mortierella alpina CFR-GV15 is a fungal culture suitable for commercial production of PUFAs with enriched EPA and DHA.

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“…6 As an alternative source, microorganisms have been used to produce EPA and DHA. 7 The n-6/n-3 fatty acid metabolic ux in microbial cells is tightly regulated by its intrinsic synthesis pathways (D6Des/ D6Elo/n3Des or D9Elo/D8Des/n3Des), and the key regulator n3Des is located at the branch point of metabolic ux. 8,9 It can convert n-6 PUFAs linoleic acid (LA, C18:2n-6), g-linoleic acid (GLA, C18:3n-6), dihomo-g-linoleic acid (DGLA, C20:3n-6) and arachidonic acid (AA, C20:4n-6) into n-3 PUFAs a-linoleic acid (ALA, C18:3n-3), stearidonic acid (STA, C18:4n-3), eicosatetraenoic acid (ETA, C20:4n-3) and eicosapentaenoic acid (EPA, C20:4n-3), respectively, and is crucial for the synthesis of n-3 long-chain PUFAs (LC-PUFAs).…”

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confidence: 99%