Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service

Teixeira, Érica Weinstein; Guimarães-Cestaro, Lubiane; Alves, Maria Elizângela Ferreira; Message, Dejair; Martins, Marta Fonseca; Luz, Cynthia Fernandes Pinto da; Serrão, José Éduardo

doi:10.1016/j.rbe.2018.04.001

Cited by 18 publications

(12 citation statements)

References 42 publications

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“…For example, a comprehensive survey on honey bee products in Brazil noted that 73.17% of the tested samples were contaminated with As. apis (compared to 87.80% from N. ceranae ) (Teixeira et al, 2018) . Although the relative severity of As.…”

Section: Discussionmentioning

confidence: 92%

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Tauber

Einspanier

Evans

et al. 2019

Preprint

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AbstractThere are viral, fungal, bacterial and trypanosomal pathogens that negatively impact the individual and superorganismal health of the western honey bee. One fungal pathogen, Ascosphaera apis, affects larvae and causes the disease chalkbrood. A previous genome analysis of As. apis revealed that its genome encodes for RNA interference genes, similar to other fungi and eukaryotes. Here, we examined whether As. apis-targeting double-stranded RNA species could disrupt the germination of As. apis. We observed that when spores were co-incubated with As. apis-targeting dsRNA, fewer spores were activated for germination, suggesting an uptake of exogenous genetic material at the very onset of germination and consequent damage to essential transcripts needed for germination. Overall, these results indicate that the causative agent of chalkbrood disease, As. apis, can be successfully targeted using an RNAi-based strategy.

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Section: Discussionmentioning

confidence: 92%

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Tauber

Einspanier

Evans

et al. 2019

Preprint

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“…They were purchased in São Paulo State, Brazil and were bottled from 2012 to 2014. Positive signs of Paenibacillus larvae were detected according to molecular analyses (Guimarães-Cestaro et al 2016, Teixeira et al 2018.…”

Section: Methodsmentioning

confidence: 99%

Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil

Luz

Guimarães-Cestaro

Serrão

et al. 2018

Hoehnea

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Royal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.

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“…The lowest infection rate was observed when the colonies' activity and population were reduced, due to lower temperatures and less availability of pollen, a product that is an important source of contamination by spores of Nosema spp. and other bee pathogens (Higes et al, 2008;Zheng et al, 2014;Guimarães-Cestaro et al, 2016;Teixeira et al, 2018). At the end of winter and early spring, with the increase of comb cleaning for posture and consequent feeding of the larvae, there was higher availability of pollen (Fig 5), higher temperatures, in addition to greater wind intensity, which possibly facilitates the spread of spores through the air (Sulborska et al, 2019), increasing prevalence of this pathogen.…”

Section: Seasonmentioning

confidence: 99%

Nosema ceranae (Microsporidia: Nosematidae) Does Not Cause Collapse of Colonies of Africanized Apis mellifera (Hymenoptera: Apidae) in Tropical Climate

et al. 2020

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Nosemosis is an important disease that affects honey bees (Apis mellifera Lineu), caused by obligate intracellular parasites, Nosema apis and/or Nosema ceranae. Since the initial detection of N. ceranae in A. mellifera coincided with recent large-scale losses of bee colonies worldwide, the impacts of this parasite under field conditions are of great interest. Here we test two hypotheses, the first one, whether the climatic variables (temperature, air humidity and precipitation) influence the intensity of infection of the microsporidium Nosema spp. in Africanized honey bees (Apis mellifera), and the second, whether the local of hive installation (outdoor or roofed) influences the intensity of infection of these spores in Africanized honey bees. Between August 2013 and August 2016, samples of Africanized bees were collected weekly from 20 colonies, of which ten were located in an open area (outdoor apiary) and ten under a roof on a concrete floor (roofed apiary). N. ceranae was the only species present. The type of apiary did not influence (p > 0.05) the number of spores of N. ceranae in Africanized bees. However, the infection intensities of the roofed apiary colonies were lower in the autumn. Regarding the meteorological parameters, there was a negative correlation between the winter infection intensities and the minimum temperature in the roofed apiary and the humidity in the outdoor apiary. The highest infection intensities occurred in both apiaries in the spring and summer, which may be related to higher pollen production. On average, the infection intensity was 16.19 ± 15.81 x 105, ranging from zero to 100.5 x105, and there were no records of collapse during the three years.

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Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service

Cited by 18 publications

References 42 publications

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil

Nosema ceranae (Microsporidia: Nosematidae) Does Not Cause Collapse of Colonies of Africanized Apis mellifera (Hymenoptera: Apidae) in Tropical Climate

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