Genetic differentiation in populations of Aedes aegypti (Diptera, Culicidae) dengue vector from the Brazilian state of Maranhão

Sousa, Andrelina Alves de; Fraga, Elmary C.; Sampaio, Iracilda; Schneider, Horácio; Barros, Maria Claudene

doi:10.1016/j.rbe.2016.10.003

Cited by 8 publications

(5 citation statements)

References 31 publications

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“…The state of Maranhão, located in Northeast Brazil, is considered one of the poorest areas of the country [29] and presents, in most municipalities, high Ae. aegypti infestation levels [30], which corroborates with several studies conducted in the region [31][32][33][34][35]. This scenario highlights the role of monitoring viral circulation in periods that precede the outbreaks, especially by entomovirological surveillance investigations.…”

Section: Introductionsupporting

confidence: 85%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

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Section: Introductionsupporting

confidence: 85%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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“…Therefore, a number of genetic markers have been developed to understand genetic variations in Aedes aegypti vectors and the genetic structure of its populations, and the phylogenetic and genetic diversity of Aedes aegypti have been characterized in different parts of the world since 1971 [ 18 ]. These markers include both biochemical and molecular tools, such as allozymes, nuclear DNA, microsatellites, and mitochondrial DNA [ 14 , 19 , 20 , 21 , 22 , 23 ]. Due to their maternal inheritance and rapid divergence, the mitochondrial DNA markers have been used extensively in phylogenetic, evolutionary, and population genetics studies of Aedes aegypti worldwide [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Distribution and Genetic Diversity of Aedes aegypti Subspecies across the Sahelian Belt in Sudan

et al. 2021

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Aedes aegypti is the most important arboviral disease vector worldwide. In Africa, it exists as two morphologically distinct forms, often referred to as subspecies, Aaa and Aaf. There is a dearth of information on the distribution and genetic diversity of these two forms in Sudan and other African Sahelian region countries. This study aimed to explore the distribution and genetic diversity of Aedes aegypti subspecies using morphology and Cytochrome oxidase-1 mitochondrial marker in a large Sahelian zone in Sudan. An extensive cross-sectional survey of Aedes aegypti in Sudan was performed. Samples collected from eight locations were morphologically identified, subjected to DNA extraction, amplification, sequencing, and analyses. We classified four populations as Aaa and the other four as Aaf. Out of 140 sequence samples, forty-six distinct haplotypes were characterized. The haplotype and nucleotide diversity of the collected samples were 0.377–0.947 and 0.002–0.01, respectively. Isolation by distance was significantly evident (r = 0.586, p = 0.005). The SAMOVA test indicated that all Aaf populations are structured in one group, while the Aaa clustered into two groups. AMOVA showed 53.53% genetic differences within populations and 39.22% among groups. Phylogenetic relationships indicated two clusters in which the two subspecies were structured. Thus, the haplotype network consisted of three clusters.

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“…Essa arbovirose pode ser causada por quatro sorotipos (DENV-1, DENV-2, DENV-3 e DENV-4). Ademais, a proximidade da espécie em relação ao homem é um fator contribuinte para que haja a ocorrência de surtos e epidemias (SOUSA et al, 2017).…”

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Torres¹,

Alves²,

Albuquerque³

et al. 2022

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A Organização Mundial da Saúde (OMS) considera as doenças negligenciadas, aquelas causadas por agentes infecciosos ou parasitas, e são endêmicas em indivíduos vulneráveis que convivem em ambiente de saneamento básico precários, água potável de má qualidade e serviços de saúde escassos. Dentre essas doenças, a malária, a doença de Chagas, a hanseníase, a leishmaniose visceral (LV), a filariose linfática, a dengue e a esquistossomose, que são consideradas as principais causas de morbidade e mortalidade em todo o mundo e no Brasil.Neste E-book abordamos uma variedade de temas, tanto sobre práticas educativas e preventivas da doença de chagas no Brasil, as dificuldades encontradas no controle da endemia de dengue, intervenções preventivas aplicadas a malária, hanseníase e a utilização da laserterapia de baixa potência na leishmaniose visceral e tegumentar americana.Agradecemos profundamente a todos os colaboradores, autores, revisores e a equipe da Luminus Editora. Desejamos a todos uma excelente leitura e esperamos contribuir para a pesquisa científica.

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Genetic differentiation in populations of Aedes aegypti (Diptera, Culicidae) dengue vector from the Brazilian state of Maranhão

Cited by 8 publications

References 31 publications

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Distribution and Genetic Diversity of Aedes aegypti Subspecies across the Sahelian Belt in Sudan

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